Abstract
The effect of lead on photosynthetic light storage efficiency was measured by the photoacoustic method. The advantage of this method is in its directness, since the signal obtained by a hydrophone is proportional to that fraction of light energy that is dissipated as heat. This increases as less energy is stored photochemically if the photosynthetic apparatus is damaged. Cells of shape Synechococcus leopoliensis (Cyanobacteria) were grown in medium containing lead in concentrations of 0, 25, 50, 100, and 200 ppm. To avoid the co-precipitation of lead and phosphorus as lead phosphate, phosphorus was omitted from the media used during weeklong exposure to lead. Both chlorophyll concentration and photosynthetic energy storage efficiency decreased with time and with lead concentration. Reduction of photosynthesis progressed with time and increased with lead concentration, reaching up to 80% at the highest lead concentration after seven days.
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References
Adam, M.S., & Abdel-Basset, R. (1990). Effect of lead nitrate and lead acetate on the growth and some metabolic processes of Scenedesmus obliquus, Acta Hydrobiologica, 32, 93–99.
Carpentier, R. (2001). The negative action of toxic divalent cations on the photosynthetic apparatus. In M. Passarakli (Ed.), Handbook of plant and crop stress (pp. 763–772). New York: Marcel Dekker.
Chaloub, R.M., de Magalhaes, C.C.P., & dos Santos, C.P. (2005). Early toxic effects of zinc on PSII of Synechocystis aquatilis f. aquatilis (Cyanophyceae). J. Phycol., 41, 1162–1168.
Croisetiere, L., Rouillon, R., & Carpentier, R. (2001). A simple mediatorless amperometric method using the cyanobacterium Synechococcus leopoliensis for the detection of phytotoxic pollutants. Applied Microbiology and Biotechnology, 56, 261–264.
Danilov, R.A., & Ekelund, N.G.A. (2001). Effects of Cu2+, Ni2+, Pb2+, Zn2+ and pentachlorophenol on photosynthesis and motility in Chlamydomonas reinhardtii in short-term exposure experiments. BMC Ecology, 1, 1.
Dubinsky, Z., Feitelson, J., & Mauzerall, D.C. (1998). Listening to phytoplankton: Measuring biomass and photosynthesis by photoacoustics. Journal of Phycology, 34, 888–892.
Fisher, T., Schurtz-Swirski, R., Gepstein, S., & Dubinsky, Z. (1989). Changes in the levels of Ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) in Tetraedron minimum (Chlorophyta) during light and shade adaptation. Plant Cell Physiology, 30, 221–228.
Fitzgerald, G.P., Gerloff, G.C., & Skoog, F. (1952). Studies on chemicals with selective toxicity to blue green algae. Sewage Ind. Wastes, 24, 888–896.
Heng, L.Y., Jusoh, K., Ling, C.H.M., & Idris, M. (2004). Toxicity of single and combinations of lead and cadmium to the Cyanobacteria anabaena flos-aquae. Bulletin of Environment Contaminant Toxicology, 72, 373–379.
Hesler, A. (1974). The effects of lead on algae. I. Effects of Pb on viability and motility of ‘Platymonas subcordiformis’ (Chlorophyta: Volvocales). Water Air Soil Pollution, 3, 371–386.
Inskeep, W.P., & Bloom, P.R. (1985). Extinction coefficients of chlorophyll a and b in N,N-dimetylformamide and 80% acetone. Plant Physiology, 77, 483–485.
Jensen, T.E., Baxter, M., Rachlin, J.W., & Jani, V. (1982a). Uptake of heavy metals by Plectonema boryanum (Cyanophyceae) into cellular components especially polyphosphate bodies: an x-ray energy dispersive study. Environ. Pollut., 27, 119–127.
Jensen, T.E., Rachlin, J.W., Jani, V., & Warkentine, B.E. (1982b). An X-ray energy dispersive study of cellular compartmentalization of lead, and zink in Chlorella saccharophila (Chlorophyta) Navicula incerta and Nitzschia closterium (Bacillariophyta). Environ. Exp. Bot., 22, 319–328.
Jensen, T.E., Rachlin, J.W., Jani, V., & Warkentine, B.E. (1986). Heavy metal uptake in relation to phosphorus nutrition in Anabaena variabilis (Cyanophyceae). Environment Polluttion, 42, 261–271.
Kelly, M. (1988). Mining and the freshwater environment. Essex, England: Elsevier Applied Science Publishers, Ltd.
Kennish, M.J. (editor) (1997). Practical handbook of estuarine and marine pollution. Boca Raton, FL: CRC Press, pp. 524.
Lewis, J. (1985). Lead poisoning: A historical perspective. Environmental Protection Agency Journal, 11, 4.
Liu, C.B., Lin, L.P., & Su, Y.C. (1996). Utilization of Chlorella vulgaris for uptake of nitrogen, phosphorus and heavy metals. Journal of the Chinese Agricultural Chemistry Society, 34, 331–343.
Malkin, S. (1996). The photoacoustic method in photosynthesis-monitoring and analysis of phenomena which lead to pressure changes following light excitation. In J. Amesz, & A. Hoff (Eds.), Biophysical techniques in photosynthesis (pp. 191–206). Kluwer Academic Publishers.
Mauzerall, D.C., Feitelson, J., & Dubinsky, Z. (1998). Discriminating between phytoplankton taxa by photoacoustics. Israel Journal of Chemistry., 38, 257–260.
Pinchasov, Y., Kotliarevsky, D., Dubinsky, Z., Mauzerall, D.C., & Feitelson, J. (2005). Photoacoustics as a diagnostic tool for probing the physiological status of phytoplankton. Israel Journal of Plant Science, 53, 1–10.
Poskuta, J.W., Parys, E., & Romanowska, E. (1996). Toxicity of lead to photosynthesis, accumulation of chlorophyll, respiration and growth of Chlorella pyrenoidosa. Protective role of dark respiration. Acta Physiology Plantarum, 18, 165–171.
Prasad, S.M., Singh, J.B., Rai, L.C., & Kumar, H.D. (1991) Metal-induced inhibition of photosynthetic electron transport chain in the cyanobacterium Nostoc muscorum. FEMS Microbiology Letters, 82, 95–100.
Rachlin, J.W., Jensen, T.E., & Warkentine, B. (1984). The growth response of the alga Anabaen flos aquae (Cyanophyceae) to cadmium copper, lead and zinc. Archives of Environment Contaminants Toxicology, 18, 143–151.
Rosko, J.J., & Rachlin, J.W. (1977). The effect of cadmium, copper, zinc and lead on cell division, growth and chlorophyll a content of the Chlorella vulgaris. Bulletin of Torrey Botony Club., 104, 226–233.
Sandau, E., Sandau, P., & Pulz, O. (1996). Heavy metals sorption by microalgae. Acta Biotechnology, 16, 227–235.
Sicko-Goad, L. (1982). A morphometric analysis of algae response to low dose, short term heavy metal exposure. Protoplasma, 110, 75–86.
Singh, J.B., Prasad, S.M., Rai, L.C., & Kumar, H.D. (1993). Response of the cyanobacterium Nostoc muscorum to chromium and lead: the effect on phosphorus metabolism. Journal of General and Applied. Microbiology, 39, 559–570.
Singh, R.P., Tripathi, R.D., Sinha, S.K., Maheshwari, R., & Srivastava, H.S. (1997). Response of higher plants to lead contaminated environment. Chemosphere, 34, 2467– 2493.
Smith, L.A., Alleman, B.C., & Copely-Graves, L. (1994). Biological treatment options. In J.L. Means, & R.E. Hinchee (eds.), Emerging technology for bioremediation of metals (pp. 1–12). Boca Raton: Lewis Publishers.
Starodub, M.E., & Wong, P.T.S. (1987). Short term and long term studies on individual and combined toxicities of copper, zinc and lead to Scenedesmus quadricauda. Science of the Total Environment, 63, 101–110.
Swift, T.D., & Forciniti, D. (1997). Accumulation of lead by Anabaena cylindrica: mathematical modeling and an energy dispersive x-ray study. Biotechnology Bioengineering., 56, 408–418.
Vymazal, J. (1990). Toxicity and accumulation of lead with respect to algae and Cyanobacteria: a review. Acta Hydrochimca et Hydrobiologica, 18, 513–533.
Wu, J.F., & Boyle, E.A. (1997). Lead in the western North Atlantic Ocean: Completed response to leaded gasoline phaseout. Geochimca et Cosmochimca Acta, 61, 3279–3283.
Zaccaro, M.C., Salazar, C., de Caire, G.Z., de Cano, M.S., & Stella, A.M. (2001). Lead toxicity in cyanobacterial porphyrin metabolism. Environmental Toxicology, 16, 61–67.
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Pinchasov, Y., Berner, T. & Dubinsky, Z. The effect of lead on photosynthesis, as determined by photoacoustics in Synechococcus leopoliensis (Cyanobacteria). Water Air Soil Pollut 175, 117–125 (2006). https://doi.org/10.1007/s11270-006-9116-z
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DOI: https://doi.org/10.1007/s11270-006-9116-z