Abstract
Typically associated with fungal species, members of the viral family Totiviridae have recently been shown to be associated with plants, including important crop species, such as Carica papaya (papaya) and Zea mays (maize). Maize-associated totivirus (MATV) was first described in China and more recently in Ecuador, where it has been found to co-occur with other viruses known to elicit maize lethal necrosis disease (MLND). In a survey for maize-associated viruses, 35 samples were selected for Illumina HiSeq sequencing, from the Tanzanian maize producing regions of Mara, Arusha, Manyara, Kilimanjaro, Morogoro and Pwani. Libraries were prepared using an RNA-tag-seq methodology. Taxonomic classification of the resulting datasets showed that 6 of the 35 samples from the regions of Arusha, Kilimanjaro, Morogoro and Mara, contained reads that were assigned to MATV reference sequences. This was confirmed with PCR and Sanger sequencing. Read assembly of the six MATV-associated datasets yielded partial MATV genomes, two of which were selected for further characterization, using RACE. This yielded two full-length MATV genomes, one of which is divergent from other available MATV genomes.
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References
Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368
Gomez-Arreaza A, Haenni A-L, Dunia I, Avilán L (2017) Viruses of parasites as actors in the parasite-host relationship: a “ménage à trois”. Acta Trop 166:123–132
de Lara Pinto AZ, de Carvalho MS, de Melo FL, Ribeiro ALM, Ribeiro BM, Slhessarenko RD (2017) Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil. PLoS ONE 12(11):e0187429
Koyama S, Urayama S, Ohmatsu T, Sassa Y, Sakai C, Takata M et al (2015) Identification, characterization, and full-length sequence analysis of a novel double-stranded RNA virus isolated from an arboreal ant, Camponotus yamaokai. J Gen Virol 96:1930–1937
Poulos BT, Tang KFJ, Pantoja CR, Bonami JR, Lightner DV (2006) Purification and characterization of infectious myonecrosis virus of penaeid shrimp. J Gen Virol 87:987–996
Antunes TFS, Amarel RJV, Ventura JA, Godinho MT, Amaral JG, Souza FO et al (2016) The dsRNA virus papaya meleira virus and an ssRNA virus are associated with papaya sticky disease. PLoS ONE 11(5):e0155240
Guo L, Yang X, Wu W, Tan G, Fang S, Zhang S (2016) Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus, family Totiviridae. Arch Virol 161:731–734
Chen S, Cao L, Huang Q, Qian Y, Zhou X (2016) The complete genome sequence of a novel maize-associated totivirus. Arch Virol 161:487–490
Alvarez-Quinto RA, Espinosa-Lozano RF, Mora-Pinargote CA, Quito-Avila DF (2017) Complete genome sequence of a variant of maize-associated totivirus from Ecuador. Arch Virol 162:1083–1087
Roosinck MJ (2018) Evolutionary and ecological links between plant and fungal viruses. New Phytol. https://doi.org/10.1111/nph.15364
Read DA, Featherston J, Rees DJG, Thompson G, Roberts R, Flett BC et al (2018) First report of maize yellow mosaic virus (MaYMV) on maize (Zea mays) in Tanzania. J Plant Pathol. https://doi.org/10.1007/s42161-018-0152-5
Shishkin AA, Giannoukos G, Kucukural A, Ciulla D, Busby M, Surka C et al (2015) Simultaneous generation of many RNA-seq libraries in a single reaction. Nat Methods 12:323–325
Menzel P, Ng KL, Krogh A (2016) Fast and sensitive taxonomic classification for metagenomics with Kaiju. Nat Commun 7:11257
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Tamura K, Stecher G, Petersen D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729
Redinbaugh MG, Stewart LR (2018) Maize lethal necrosis: an emerging, synergistic viral disease. Annu Rev Virol 5:301–322
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DJGR, BCF and KM contributed to the study design. DAR, JF, GDT, RR, GP, BK, AK and ERM contributed to various aspects of sample collection. DAR, GDT, RR and GP wrote the manuscript.
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Read, D.A., Featherston, J., Rees, D.J.G. et al. Diversity and distribution of Maize-associated totivirus strains from Tanzania. Virus Genes 55, 429–432 (2019). https://doi.org/10.1007/s11262-019-01650-6
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DOI: https://doi.org/10.1007/s11262-019-01650-6