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The complete genome sequence of a novel maize-associated totivirus

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Abstract

Deep sequencing of small RNA (sRNA) populations in maize plants from southwest China resulted in the identification of a previously unknown dsRNA virus with a sequence and genome organization resembling that of a totivirus. The complete viral genome is 3,956 nucleotides in length and contains two open reading frames (ORFs) with the potential to produce a ORF1-ORF2 fusion protein through a -1 ribosomal frameshift translation mechanism. ORF1 encodes the putative capsid protein (CP), whereas the predicted product of ORF2 contains motifs typical of an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis using the amino acid sequences of putative RdRp fusion proteins showed that the new virus was grouped in a clade together with the totiviruses, suggesting that it is a new member of the genus Totivirus of the family Totiviridae. The virus is tentatively named “maize-associated totivirus (MATV)”. Our findings demonstrate that it is feasible to identify totiviruses by deep sequencing of small RNAs.

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Acknowledgments

This research was supported by a foundation for detecting plant virus using next-generation sequencing (grant 201310068) from General Administration of Quality Supervision, Inspection and Quarantine of China.

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, People’s Republic of China

    Sha Chen, Linge Cao, Qingqing Huang, Yajuan Qian & Xueping Zhou

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  1. Sha Chen
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  2. Linge Cao
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  3. Qingqing Huang
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  4. Yajuan Qian
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  5. Xueping Zhou
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Correspondence to Yajuan Qian.

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Chen, S., Cao, L., Huang, Q. et al. The complete genome sequence of a novel maize-associated totivirus. Arch Virol 161, 487–490 (2016). https://doi.org/10.1007/s00705-015-2657-y

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  • DOI: https://doi.org/10.1007/s00705-015-2657-y

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