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Molecular genetic analysis and evolution of begomoviruses and betasatellites causing yellow mosaic disease of bhendi

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Abstract

In India, Bhendi yellow vein mosaic disease (BYVMD) is one of the most economically important diseases of bhendi/okra and is caused by a complex of monopartite begomovirus (Bhendi yellow vein mosaic virus—BYVMV) and betasatellite (Bhendi yellow vein betasatellite—BYVB). In this study, we have analyzed the role of possible evolutionary factors involved in the evolution of BYVMV and BYVB isolates. Evidence of inter-species and inter-strain recombination events was detected among the viral isolates, and majority of these recombinant isolates possess microsatellites in their genome. Recombination analysis suggests that cotton-infecting and bhendi-infecting begomoviruses probably share a recent common ancestor. In addition to genetic differentiation and gene flow, high degree of genetic variability was detected among the viral population. A strong purifying selection seems to be acting on the viral coding regions. The nucleotide substitution rate of V1 gene (for BYVMV) and βC1 gene (for BYVB) was estimated to be 7.55 × 10−4 and 2.25 × 10−3 nucleotide substitutions/site/year, respectively. The present study underlines that the evolution of BYVMD-associated viral components is driven by selection acting on the genetic variation generated by recombination and mutation.

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Acknowledgements

SC gratefully acknowledges the Department of Biotechnology, Government of India, for the financial support through a Research Grant (BT/SBIRI/571/23-B11/2009).

Author’s contributions

RV and SC conceived the study, analyzed data, and drafted the article. RV, DR, and AKS performed the experiments. SC, HCP, and GKG contributed reagents/materials/analysis tools. All the authors had read and approved the article.

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Author notes

  1. A. K. Singh

    Present address: Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, India

Authors and Affiliations

  1. Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India

    R. Vinoth Kumar, A. K. Singh, D. Ragunathan & S. Chakraborty

  2. Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, India

    H. C. Prasanna

  3. Krishidhan Research Foundation Private Limited, Jalna, Maharashtra, India

    G. K. Garg

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  1. R. Vinoth Kumar
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Correspondence to S. Chakraborty.

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Kumar, R.V., Prasanna, H.C., Singh, A.K. et al. Molecular genetic analysis and evolution of begomoviruses and betasatellites causing yellow mosaic disease of bhendi. Virus Genes 53, 275–285 (2017). https://doi.org/10.1007/s11262-016-1414-y

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