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Sequencing and phylogenetic analysis of an avian reovirus genome

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Abstract

Avian reovirus infection causes considerable economic loss to the commercial poultry industry. Live-attenuated vaccine strain S1133 (v-S1133, derived from parent strain S1133) is considered the safest and most effective vaccine and is currently used worldwide. To identify the genes responsible for its attenuation, DNA sequences of open reading frames (ORF) of S1133 and its parent strains S1133, 1733, 526, and C78 along with three field isolates (GuangxiR1, GuangxiR2, and GX110058) and one isolate (GX110116) from a vaccinated chicken were performed. The sequence data were compared with available sequences in nucleotide sequence databases of American (AVS-B, 138, 176) and Chinese (C-98 and T-98) origin. Sequence analysis identified that several v-S1133 specific nucleotide substitutions existed in the ORFs of λA, λB, λC, μA, μB, μNS, σA, σB, and σNS genes. The v-S1133 strain could be differentiated from the field-isolated strains based on single nucleotide polymorphisms. Phylogenetic analysis revealed that v-S1133 shared the highest sequence homologies with S1133 and reovirus isolates from China, grouped together in one cluster. Chinese isolates were clearly more distinct from the American reovirus AVS-B strain, which is associated with runting–stunting syndrome in broilers.

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Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (no.31160512), Guangxi Science and Technology Bureau (no.1222003-2-4), and Guangxi Government Senior Scientist Foundation (no.2011B020). The authors thank Drs. Salvatore Frasca Jr. and Kirklyn Kerr for their critical review of the manuscript.

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Authors and Affiliations

  1. Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, 51 You Ai North Road, Nanning, 530001, Guangxi, China

    Liqiong Teng, Zhixun Xie, Liji Xie, Jiabo Liu, Yaoshan Pang, Xianwen Deng, Zhiqin Xie, Qing Fan & Sisi Luo

  2. Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China

    Jiaxun Feng

  3. Department of Pathobiology & Veterinary Science, University of Connecticut, 61 North Eagleville Road, Storrs, CT, 06269-3089, USA

    Mazhar I. Khan

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  1. Liqiong Teng
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  2. Zhixun Xie
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  3. Liji Xie
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  4. Jiabo Liu
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  5. Yaoshan Pang
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  7. Zhiqin Xie
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  8. Qing Fan
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  9. Sisi Luo
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  10. Jiaxun Feng
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  11. Mazhar I. Khan
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Correspondence to Zhixun Xie or Mazhar I. Khan.

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Teng, L., Xie, Z., Xie, L. et al. Sequencing and phylogenetic analysis of an avian reovirus genome. Virus Genes 48, 381–386 (2014). https://doi.org/10.1007/s11262-013-1029-5

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  • DOI: https://doi.org/10.1007/s11262-013-1029-5

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