Skip to main content
SpringerLink
Log in

Molecular epidemiological investigation of velogenic Newcastle disease viruses from village chickens in Cambodia

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Three isolates of Newcastle disease virus (NDV) were isolated from tracheal samples of dead village chickens in two provinces (Phnom Penh and Kampong Cham) in Cambodia during 2011–2012. All of these Cambodian NDV isolates were categorized as velogenic pathotype, based on in vivo pathogenicity tests and F cleavage site motif sequence (112RRRKRF117). The phylogenetic analysis and the evolutionary distances based on the sequences of the F gene revealed that all the three field isolates of NDV from Cambodia form a distinct cluster (VIIh) together with three Indonesian strains and were assigned to the genotype VII within the class II. Further phylogenetic analysis based on the hyper-variable region of the F gene revealed that some of NDV strains from Malaysia since the mid-2000s were also classified into the VIIh virus. This indicates that the VIIh NDVs are spreading through Southeast Asia. The present investigation, therefore, emphasizes the importance of further surveillance of NDV in neighboring countries as well as throughout Southeast Asia to contain further spreading of these VIIh viruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. M.A. Mayo, Arch. Virol. 147, 1071–1076 (2002)

    Article  PubMed  CAS  Google Scholar 

  2. C.L. Afonso, P.J. Miller, C. Grund, G. Koch, B. Peeters, P.W. Selleck, G.B. Srinivas, in Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees). 7th edn, ed. by World Organization for Animal Health (OIE, Paris, 2012), pp. 555–573

  3. D.J. Alexander, in Diseases of Poultry, by Y.M. Saif, H.J. Barnes, J.R. Glisson, A.M. Fadly, L.R. McDougald, D.E. Swayne (Iowa State Press, Ames, 2003), pp. 63–99

  4. M.S. Collins, J.B. Bashiruddin, D.J. Alexander, Arch. Virol. 128, 363–370 (1993)

    Article  PubMed  CAS  Google Scholar 

  5. Y. Nagai, Trends Microbiol. 1, 81–87 (1993)

    Article  PubMed  CAS  Google Scholar 

  6. A. Panda, Z. Huang, S. Elankumaran, D.D. Rockemann, S.K. Samal, Microb. Pathog. 36, 1–10 (2004)

    Article  PubMed  CAS  Google Scholar 

  7. L.M. Kim, D.J. King, D.L. Suarez, C.W. Wong, C.L. Afonso, J. Clin. Microbiol. 45, 1310–1314 (2007)

    Article  PubMed  CAS  Google Scholar 

  8. C. Meng, X. Qiu, S. Jin, S. Yu, H. Chen, C. Ding, Arch. Virol. 157, 869–880 (2012)

    Article  PubMed  CAS  Google Scholar 

  9. P.J. Miller, E.L. Decanini, C.L. Afonso, Infect. Genet. Evol. 10, 26–35 (2010)

    Article  PubMed  CAS  Google Scholar 

  10. D.G. Diel, L.H. da Silva, H. Liu, Z. Wang, P.J. Miller, C.L. Afonso, Infect. Genet. Evol. 12, 1770–1779 (2012)

    Article  PubMed  Google Scholar 

  11. P.J. Miller, L.M. Kim, H.S. Ip, C.L. Afonso, Virology 391, 64–72 (2009)

    Article  PubMed  CAS  Google Scholar 

  12. S. Sen, S.M. Shane, D.T. Scholl, M.E. Hugh-Jones, J.M. Gillespie, Prev. Vet. Med. 35, 283–295 (1998)

    Article  PubMed  CAS  Google Scholar 

  13. P.B. Spradbrow, Poult. Sci. Rev. 5, 57–96 (1994)

    Google Scholar 

  14. T.D. Tu, K.V. Phuc, N. Thi, K. Dinh, D.N. Quoc, P.B. Spradbrow, Prev. Vet. Med. 34, 205–214 (1998)

    Article  PubMed  CAS  Google Scholar 

  15. K.S. Choi, S.J. Kye, J.Y. Kim, H.S. Lee, Avian Dis. (2013, in press)

  16. D.J. Alexander,. in A Laboratory Manual for the Isolation and Identification of Avian Pathogens, 5th edn, ed. by D.E. Swayne, J.R. Glisson, M.W. Jackwood, J.E. Pearson, W.M. Reed (American Association of Avian Pathologists, Athens, 2008), pp. 156–163

  17. Y.J. Lee, H.W. Sung, J.G. Choi, J.H. Kim, C.S. Song, Avian Pathol. 33, 482–491 (2004)

    Article  PubMed  CAS  Google Scholar 

  18. M. Kimura, J. Mol. Evol. 16, 111–120 (1980)

    Article  PubMed  CAS  Google Scholar 

  19. K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731–2739 (2011)

    Article  PubMed  CAS  Google Scholar 

  20. E.W. Aldous, J.K. Mynn, J. Banks, D.J. Alexander, Avian Pathol. 32, 239–256 (2003)

    Article  PubMed  CAS  Google Scholar 

  21. G.M Ke, S.W. Yu, C.H. Ho, P.Y. Chu, L.Y. Ke, K.H. Lin, Y.C. Tsai, H.J. Liu, M.Y. Lin, Virus Res. 147, 247–257 (2010)

    Google Scholar 

  22. X.F. Liu, H.Q. Wan, X.X. Ni, Y.T. Wu, W.B. Liu, Arch. Virol. 148, 1387–1403 (2003)

    PubMed  CAS  Google Scholar 

  23. M.M. Ebrahimi, S. Shahsavandi, G. Moazenijula, M. Shamsara, Virus Genes 45, 63–68 (2012)

    Article  PubMed  CAS  Google Scholar 

  24. S.W. Tan, A. Ideris, A.R. Omar, K. Yusoff, M. Hair-Bejo, Arch. Virol. 155, 63–70 (2010)

    Article  PubMed  CAS  Google Scholar 

  25. L. Yu, Z. Wang, Y. Jiang, L. Chang, J. Kwang, J. Clin. Microbiol. 39, 3512–3519 (2001)

    Article  PubMed  CAS  Google Scholar 

  26. S. Xiao, A. Paldurai, B. Nayak, A. Samuel, E.E. Bharoto, T.Y. Prajitno, P.L. Collins, S.K. Samal, J. Virol. 86, 5969–5970 (2012)

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was fully supported by the Animal and Plant Quarantine Agency, Republic of Korea (Grant No. M-AD15-2011-13-02).

Author information

Authors and Affiliations

  1. Avian Diseases Division, Animal and Plant Quarantine Agency, MAFRA, 175 Anyang-ro, Anyang, Gyeonggi, 430-757, Republic of Korea

    Kang-Seuk Choi, Soo-Jeong Kye, Ji-Ye Kim, Vanessa R. Damasco, Youn-Jeong Lee, Jun-Gu Choi, Hyun-Mi Kang, Kwang-Il Kim, Byung-Min Song & Hee-Soo Lee

  2. Ministry of Agriculture, Forestry and Fisheries, National Veterinary Institute, Phnom Penh, Cambodia

    San Sorn

Authors
  1. Kang-Seuk Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Soo-Jeong Kye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ji-Ye Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vanessa R. Damasco
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. San Sorn
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Youn-Jeong Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jun-Gu Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hyun-Mi Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Kwang-Il Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Byung-Min Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Hee-Soo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kang-Seuk Choi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Choi, KS., Kye, SJ., Kim, JY. et al. Molecular epidemiological investigation of velogenic Newcastle disease viruses from village chickens in Cambodia. Virus Genes 47, 244–249 (2013). https://doi.org/10.1007/s11262-013-0930-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-013-0930-2

Keywords

Search

Navigation