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Whole genome sequencing and biological characterization of Duck/JS/10, a new lentogenic class I Newcastle disease virus

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Abstract

A lentogenic Newcastle disease virus (NDV), Duck/JS/10 (JS10), was isolated from an unvaccinated duck in China. The complete genome of the virus contained 15,198 nucleotides. Based on length of the genome and a partial sequence of the F gene, the virus was classified as a class I genotype 4 NDV. The antigenicity of the virus was compared with that of NDV strain La Sota via hemagglutination inhibition (HI), virus neutralization (VN) assay and animal experiments. Our results show that JS10 generates higher HI and VN titers than La Sota against both class I and II virulent NDV strains. Experiments on animals demonstrate that virus shedding from chickens vaccinated with JS10 is significantly reduced when compared to those vaccinated with La Sota. Overall, this study strongly suggests that JS10 may qualify as a new vaccine candidate against Newcastle disease.

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Acknowledgments

We thank Professor Xiufan Liu from Yangzhou University, China, and Professors Toshihiro Ito and Koichi Otsuki from Tottori University, Japan, for providing us with NDV strains. This work was funded by Chinese Special Fund for Agro-scientific Research in the Public Interest (201003012) and Chinese National High-tech R&D Program (863 Program, 2011AA10A209).

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Authors and Affiliations

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, 200241, People’s Republic of China

    Chunchun Meng, Xvsheng Qiu, Shiqiang Jin, Shengqing Yu, Hongjun Chen & Chan Ding

  2. Key Laboratory of Environmental Hazard and Animal Diseases of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, 625014, People’s Republic of China

    Shiqiang Jin

  3. China National Engineering Technology Research Centre for Poultry, 2949 Zhennan Road, Shanghai, 200331, People’s Republic of China

    Chan Ding

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  1. Chunchun Meng
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  2. Xvsheng Qiu
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Correspondence to Chan Ding.

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Meng, C., Qiu, X., Jin, S. et al. Whole genome sequencing and biological characterization of Duck/JS/10, a new lentogenic class I Newcastle disease virus. Arch Virol 157, 869–880 (2012). https://doi.org/10.1007/s00705-012-1248-4

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  • DOI: https://doi.org/10.1007/s00705-012-1248-4

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