Skip to main content
SpringerLink
Log in

Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

In this study, the complete genome sequence of a Newcastle disease virus (NDV) isolate collected from an outbreak in 1995 in chickens was fully characterized and compared with other NDV sequences. The genome was found to be 15,192 nucleotides long and to consist of six genes in the order 3′-NP-P-M-F-HN-L-5′, similar to other avian paramyxoviruses type-I. However, a six-nucleotide insertion was observed in the 5′ non-coding regions of the nucleoprotein (NP) gene, a feature that is unique to some NDV isolates. The isolate shows the amino acid sequence 112RRQKRF117 at the cleavage site of the F protein, which is identical to a known motif for virulent pathotypes of NDV. The phylogenetic analysis of the coding region of the F gene indicated that this isolate belongs to genotype VI, more specifically to genotype VId, along with isolates from the other European countries (Denmark, Switzerland and Austria). The same genotype caused outbreaks in the Middle East and Greece in the late 1960s, and in Hungary, in the early 1980s, suggesting a common source for these outbreaks.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. F.C. Kraneveld, Ned Indisch Bl Diergeneeskd 38, 448–450 (1926)

    Google Scholar 

  2. T.M. Doyle, J. Comp. Path. Therap. 40, 144–169 (1927)

    Google Scholar 

  3. D.J. Alexander, in Diseases of poultry, 11th edn., ed. by Y.M. Saif, J.BarnesH, J.R. Glisson, A.M. Fadly, L.R. McDougal, D.E. Swayne (Iowa State Press, Ames, 2003), pp. 64–874

  4. OIE, in Newcastle Disease. ed. by M. Truszczynski et al. International Animal Health Code—Mammals, Birds and Bees, 6th edn. in Epizooties (OIE, Paris, 1992), pp. 157–165

  5. M.A. Mayo, Arch. Virol. 147, 1071–1076 (2002)

    Article  CAS  Google Scholar 

  6. D. Kolakofsky, T. Pelet, D. Garcin, S. Hausmann, J. Curran, L. Roux, J. Virol. 72, 891–899 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  7. X.F. Liu, H.Q. Wan, X.X. Ni, Y.T. Wu, W.B. Liu, Arch. Virol. 148, 1387–1403 (2003)

    CAS  PubMed  Google Scholar 

  8. D. Kolakofsky, L. Roux, D. Garcin, R.W. Ruigrok, J. Gen. Virol. 86, 1869–1877 (2005)

    Article  CAS  Google Scholar 

  9. M. Mase, K. Imai, Y. Sanada, N. Sanada, N. Yuasa, T. Imada, K. Tsukamoto, S. Yamaguchi, J. Clin. Microbiol. 40, 3826–3830 (2002)

    Article  CAS  Google Scholar 

  10. D.J. Alexander, Newcastle disease diagnosis, in Newcastle disease, ed. by D.J. Alexander (Kluwer Academic Publishers, Boston, 1988)

    Chapter  Google Scholar 

  11. D. Wei, B. Yang, Y.L. Li, C.F. Xue, Z.N. Chen, H. Bian, Virus Res. 135, 312–319 (2008)

    Article  CAS  Google Scholar 

  12. J.C.F.M. Dortmans, G. Koch, P.J.M. Rottier, B.P.H. Peeters, J. Gen. Virol. 90, 2746–2750 (2009)

    Article  CAS  Google Scholar 

  13. D.J. Alexander, Br. Poultry Sci. 42, 5 (2001)

    Article  CAS  Google Scholar 

  14. D.J. Alexander, in Newcastle disease and other avian paramyxoviridae infections. ed. by B.W. Calnek, H.J. Barnes, C.W. Beard, L.R. McDougald, Y.M. Saif. Diseases of Poultry (Iowa State Press, Ames, 1997), pp. 541–569

  15. M. Munir, A.M. Linde, S. Zohari, K. Stahl, C. Baule, K. Holm, B. Engstrom, M. Berg, Avian Dis. 54, 923–930 (2010)

    Article  Google Scholar 

  16. K. Tamura, J. Dudley, M. Nei, S. Kumar, Mol. Biol. Evol. 24, 1596–1599 (2007)

    Article  CAS  Google Scholar 

  17. D. Ujvari, Virus Genes 32, 49–57 (2006)

    Article  CAS  Google Scholar 

  18. R. Lamb, G. Parks, Paramyxoviridae: The Viruses and Their Replication (Lippincott Williams & Wilkins, Philadelphia, 2007)

    Google Scholar 

  19. E.W. Aldous, J.K. Mynn, J. Banks, D.J. Alexander, Avian Pathol. 32, 239–256 (2003)

    Article  CAS  Google Scholar 

  20. B. Rima, Comparison of amino acid sequences of the major structural proteins of the paramyxo- and morbilliviruses, in Genetics and Pathogenicity of Negative Strand Viruses, ed. by D. Kolakofsky, B.M.J. Mahy (Elsevier, Amsterdam, 1989)

    Google Scholar 

  21. T. Sakaguchi, T. Toyoda, B. Gotoh, N.M. Inocencio, K. Kuma, T. Miyata, Y. Nagai, Virology 169, 260–272 (1989)

    Article  CAS  Google Scholar 

  22. Y. Nagai, H.D. Klenk, Virology 77, 125–134 (1977)

    Article  CAS  Google Scholar 

  23. A. Czegledi, D. Ujvari, E. Somogyi, E. Wehmann, O. Werner, B. Lomniczi, Virus Res. 120, 36–48 (2006)

    Article  CAS  Google Scholar 

  24. B. Lomniczi, E. Wehmann, J. Herczeg, A. Ballagi-Pordany, E.F. Kaleta, O. Werner, G. Meulemans, P.H. Jorgensen, A.P. Mante, A.L. Gielkens, I. Capua, J. Damoser, Arch. Virol. 143, 49–64 (1998)

    Article  CAS  Google Scholar 

  25. A.M. Linde, M. Munir, S. Zohari, K. Stahl, C. Baule, L. Renstrom, M. Berg, Virus Genes 41, 165–173 (2010)

    Article  CAS  Google Scholar 

  26. I.H. Brown, L. Henry, VLA report (2009/10)

  27. B.S. Seal, Virus Genes 11, 217–224 (1996)

    Article  Google Scholar 

  28. A.R. Gould, E. Hansson, K. Selleck, J.A. Kattenbelt, M. Mackenzie, A.J. Della-Porta, Avian Pathol. 32, 361–373 (2003)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the European Union Network of Excellence, EPIZONE (Contract No. FOOD-CT-2006-016236), and the Swedish International Development Cooperation Agency, Department for Research Cooperation (Sida, SAREC: Contract No. SWE- 2008-186). The authors also would like to thank Jenna Anderson for English proofreading of this article.

Author information

Authors and Affiliations

  1. The Department of Biomedical Sciences and Veterinary Public Health, Division of Virology and Parasitology and Joint Research and Development, Department of Virology, Immunobiology and Parasitology of the National Veterinary Institute (SVA) and Swedish University of Agricultural Sciences (SLU), Ulls väg 2B, 750 071 89, Uppsala, Sweden

    Muhammad Munir, Anna-Malin Linde, Siamak Zohari, Karl Ståhl & Mikael Berg

  2. Department of Virology, Immunobiology and Parasitology, National Veterinary Institute (SVA), Ulls väg 2B, 751 89, Uppsala, Sweden

    Siamak Zohari, Claudia Baule & Lena H. M Renström

  3. Department of Animal Health and Antimicrobial Strategies of the National Veterinary Institute (SVA), Ulls väg 2B, 751 89, Uppsala, Sweden

    Björn Engström

Authors
  1. Muhammad Munir
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anna-Malin Linde
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Siamak Zohari
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Karl Ståhl
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Claudia Baule
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Björn Engström
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lena H. M Renström
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Mikael Berg
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Muhammad Munir.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Munir, M., Linde, AM., Zohari, S. et al. Whole genome sequencing and characterization of a virulent Newcastle disease virus isolated from an outbreak in Sweden. Virus Genes 43, 261–271 (2011). https://doi.org/10.1007/s11262-011-0636-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-011-0636-2

Keywords

Search

Navigation