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Molecular detection of genogroup I sapovirus in Tunisian children suffering from acute gastroenteritis

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Abstract

This study investigated the prevalence of sapovirus infections in children with acute gastroenteritis in Monastir region, Tunisia, from January 2003 to April 2007. Sapovirus was characterized by sequence and phylogenetic analyses of the partial polymerase gene. From 788 fecal specimens tested, 6 (0.8%) were positive for sapovirus, of these, 4 (66.7%) were monoinfections. All sapovirus positive samples were detected in outpatient, contrary to norovirus which was significantly more frequent in hospitalized children than in outpatients (14.5 vs. 9.5%, P = 0.03). The mean age of children with sapovirus infections was 11 ± 5.56 months (range 6–19 months). Sapovirus isolates were detected in March and between September and December 2003. Fever, vomiting, abdominal pain, and dehydration were not observed in patients with sapovirus infections. Analysis of nucleotide and amino acid sequences revealed that all 6 Tunisian sapovirus strains clustered in the GGI/1 genotype and strains were identical in the region sequenced, sharing 90.2% nucleotide identity with the reference strain Sapporo/82/JP (U65427). This represents the first finding of sapovirus infections in North Africa and especially in Tunisia. The data indicate that, contrary to norovirus which can cause severe diarrhea and is an important etiologic agent in hospitalized cases, sapovirus causes mild gastroenteritis in Tunisian children.

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Acknowledgments

The study was partly supported by the CMCU project (code 04/S0813) and the National Reference Center (NRC) for Enteric Viruses (Dijon, France). The authors thank Maha Mastouri from the Laboratory of Microbiology of the University Hospital Fattouma-Bourguiba for helping to collect stool samples.

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Authors and Affiliations

  1. Laboratory of Infectious Diseases and Biological Agents, Faculty of Pharmacy, 5000, Monastir, Tunisia

    Khira Sdiri-Loulizi, Mouna Hassine, Hakima Gharbi-Khelifi, Zaidoun Aouni & Mahjoub Aouni

  2. National Reference Center for Enteric Viruses, Laboratory of Virology, University Hospital of Dijon, 2 Rue Angélique Ducoudray, 37013, 21070, Dijon, France

    Khira Sdiri-Loulizi, Pierre Pothier & Katia Ambert-Balay

  3. Department of Pediatric, University Hospital Fattouma Bourguiba, 5000, Monastir, Tunisia

    Slaheddine Chouchane & Mohamed Neji-Guédiche

  4. Laboratory of Immunology, University Hospital Fattouma Bourguiba, 5000, Monastir, Tunisia

    Nabil Sakly

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  1. Khira Sdiri-Loulizi
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  2. Mouna Hassine
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Correspondence to Khira Sdiri-Loulizi.

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Sdiri-Loulizi, K., Hassine, M., Gharbi-Khelifi, H. et al. Molecular detection of genogroup I sapovirus in Tunisian children suffering from acute gastroenteritis. Virus Genes 43, 6–12 (2011). https://doi.org/10.1007/s11262-011-0600-1

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