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Sequence analysis of VP2 gene of canine parvovirus isolates in Thailand

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Abstract

Canine parvovirus (CPV) causes a very severe enteric disease especially in puppies. Twenty-six isolates of CPV were obtained from dogs at the Animal Hospital, Kasetsart University, Thailand. Whole VP2 gene of 26 isolates was amplified using polymerase chain reaction (PCR) and its sequences were analyzed. Nineteen out of 26 isolates were characterized as CPV type 2a variants and the rest of the isolates were characterized as CPV type 2b. These results indicated that both types are currently prevalent field CPV circulating in Thailand and type 2a is the predominant genotype. Neither CPV type 2 nor type 2c was observed in this study.

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Acknowledgments

This research was supported by grant from the program Strategic Scholarships for Frontier Research Network for the Ph.D. Program Thai Doctoral degree from the Commission on Higher Education, Thailand, the Center for Agricultural Biotechnology, Postgraduate Education and Research Development Office, Commission on Higher Education, Ministry of Education and Uttaradit Rajabhat University. We thank P. Sangthong (Department of Genetics, Faculty of Science, Kasetsart University) for providing consultation about bioinformatics and R. Sinsiri (Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Kasetsart University) for providing technical support and specimens.

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Authors and Affiliations

  1. Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakorn Pathom, 73140, Thailand

    Siriwadee Phromnoi, Kaitkanoke Sirinarumitr & Theerapol Sirinarumitr

  2. Center for Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Nakorn Pathom, 73140, Thailand

    Siriwadee Phromnoi, Kaitkanoke Sirinarumitr & Theerapol Sirinarumitr

  3. Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand

    Kaitkanoke Sirinarumitr

  4. Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand

    Theerapol Sirinarumitr

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  1. Siriwadee Phromnoi
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Correspondence to Theerapol Sirinarumitr.

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Phromnoi, S., Sirinarumitr, K. & Sirinarumitr, T. Sequence analysis of VP2 gene of canine parvovirus isolates in Thailand. Virus Genes 41, 23–29 (2010). https://doi.org/10.1007/s11262-010-0475-6

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  • DOI: https://doi.org/10.1007/s11262-010-0475-6

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