Abstract
Enterovirus 71 (EV71) strains from children were characterized by full-length VP1 nucleotide sequencing. Out of 22 clinical specimens, five isolates identified as EV71 were recovered by virus isolation. The VP1 sequences of the five isolates had more than 97.4% sequence identity with prototype virus BrCr, clustering in the genotype A lineage. This represents the first record of genotype A EV71 in China since the BrCr prototype strain was discovered in the USA in 1969.
Abbreviations
- EV:
-
Enterovirus
- HFMD:
-
Hand, foot, and mouth disease
- CA16:
-
Coxsackievirus A16
- RT-PCR:
-
Reverse transcription-polymerase chain reaction
- CSF:
-
Cerebrospinal fluid
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Acknowledgments
We are grateful to Dr. Wen Hu (Department of Pathology, Provincial Affiliated Hospital, Anhui Medical University) for his technical assistance in electron microscopy, and also express sincere thanks to Dr. Qiong Wang (Department of Biochemistry and Molecular Biology, Baylor College of Medicine, USA) and Dr. Jie Lu (School of Medicine, University of Pennsylvania, USA) for critical reading and significant suggestions. This work was supported by a grant from the general research program of Anhui Science and Technology Department, Anhui Province, P. R. China (No. 08010302179).
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The GenBank accession numbers of the sequences reported in this article are GQ117124–GQ117128.
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Yu, H., Chen, W., Chang, H. et al. Genetic analysis of the VP1 region of enterovirus 71 reveals the emergence of genotype A in central China in 2008. Virus Genes 41, 1–4 (2010). https://doi.org/10.1007/s11262-010-0472-9
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DOI: https://doi.org/10.1007/s11262-010-0472-9