Abstract
Expression of the human immunodeficiency virus type 1 (HIV-1) Rev protein is essential for completion of the viral life cycle. Rev mediates nuclear export of partially spliced and unspliced viral transcripts and therefore bears a nuclear localization signal (NLS) as well as a nuclear export signal (NES), which allow its nucleocytoplasmic shuttling. Attempts to express the wild-type Rev protein in eukaryotic human cultured cells have encountered difficulties and so far have failed. Here we show that accumulation of Rev, which occurs in nondividing Rev-expressing cells or when such cells reach confluency, results in death of these cells. Cell death was also promoted by addition of a cell permeable peptide bearing the Rev-NES sequence, but not by the Rev-NLS peptide. Our results probably indicate that binding of excess amounts of the Rev protein or the NES peptide to the exportin receptor CRM1 results in cells’ death.
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This study was supported by the Israeli Science Foundation (AL) and by a starting grant from the European Research Council (ERC) (to AF).
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Levin, A., Hayouka, Z., Friedler, A. et al. Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cells. Virus Genes 40, 341–346 (2010). https://doi.org/10.1007/s11262-010-0458-7
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DOI: https://doi.org/10.1007/s11262-010-0458-7