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Detection of non-polio enteroviruses in Hungary 2000–2008 and molecular epidemiology of enterovirus 71, coxsackievirus A16, and echovirus 30

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Abstract

Human enteroviruses are associated with various clinical syndromes from minor febrile illness to severe, potentially fatal conditions like aseptic meningitis, paralysis, myocarditis, and neonatal enteroviral sepsis. Between June 2000 and August 2008 echovirus (E) type 2, 4, 6, 7, 9, 11, 13, 25, 30, coxsackievirus (CV) -A16, -A19, -B5, and enterovirus 71 (EV71) were reported in Hungary. In this study, 29 previously enterovirus positive samples from 28 patients diagnosed with hand, foot and mouth disease, meningitis and encephalitis, were molecularly typed. The genetic relationships of identified serotypes CV-A16, EV71, and E30 were assessed by direct sequencing of genomic region encoding the capsid protein VP1. The sequences were compared to each other and sequences from other geographical regions possessed in Genbank. The phylogenetic analysis of CV-A16 revealed that the viruses were mostly of Far-Eastern or Asia-Pacific origin. Typing of EV71 showed that one virus from 2000 belonged to genotype C1 and five viruses observed in 2004 and 2005 were identified as genotype C4. The 11 echovirus 30 strains showed homology with those of neighbor European countries. The molecular examination of E30 revealed that three separate lineages circulated in 2000, 2001, and 2004–2006 in Hungary.

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References

  1. H.T. Wright Jr., B.H. Landing, E.H. Lennette, R.M. Mcallister, Fatal infection in an infant associated with Coxsackie virus group A, type 16. N. Engl. J. Med. 9(268), 1041–1044 (1963)

    Article  Google Scholar 

  2. R. Chemez, G. Nagy, C. Németh, I. Dömök, Hand, foot and mouth disease epidemic in a nursery. Orv. Hetil. 117, 2421–2423 (1976)

    CAS  PubMed  Google Scholar 

  3. G. Nagy, S. Takatsy, E. Kukan, I. Mihaly, I. Domok, Virological diagnosis of enterovirus type 71 infections: experiences gained during an epidemic of acute CNS diseases in Hungary in 1978. Arch. Virol. 71, 217–227 (1982)

    Article  CAS  PubMed  Google Scholar 

  4. L.W. Ang, B.K. Koh, K.P. Chan, L.T. Chua, L. James, K.T. Goh, Epidemiology and control of hand, foot and mouth disease in Singapore, 2001–2007. Ann. Acad. Med. Singap. 38(2), 106–112 (2009)

    PubMed  Google Scholar 

  5. K.B. Chua, B.H. Chua, C.S. Lee, Y.K. Chem, N. Ismail, A. Kiyu, V. Kumarasamy, Genetic diversity of enterovirus 71 isolated from cases of hand, foot and mouth disease in the 1997, 2000 and 2005 outbreaks, Peninsular Malaysia. Malays. J. Pathol. 29(2), 69–78 (2007)

    CAS  PubMed  Google Scholar 

  6. T. Hamaguchi, H. Fujisawa, K. Sakai, S. Okino, N. Kurosaki, Acute encephalitis caused by intrafamilial transmission of enterovirus 71 in adult. Emerg. Infect. Dis. 14(5), 828–830 (2008)

    Article  PubMed  Google Scholar 

  7. J.R. Wang, Y.C. Tuan, H.P. Tsai, J.J. Yan, C.C. Liu, I.J. Su, Change of major genotype of enterovirus 71 in outbreaks of hand-foot-and-mouth disease in Taiwan between 1998 and 2000. J. Clin. Microbiol. 40(1), 10–15 (2000)

    Article  Google Scholar 

  8. B.A. Brown, M.S. Oberste, J.P. Alexander Jr., M.L. Kennett, M.A. Pallansch, Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998. J Virol. 73(12), 9969–9975. Erratum in: J Virol. 2000; 74(24), 12003 (1999)

    Google Scholar 

  9. J.M. Bible, M. Iturriza-Gomara, B. Megson, D. Brown, P. Pantelidis, P. Earl, J. Bendig, C.Y.W. Tong, Molecular epidemiology of human enterovirus 71 in the United Kingdom from 1998 to 2006. J. Clin. Microbiol. 46(10), 3192–3200 (2006)

    Article  CAS  Google Scholar 

  10. S. Diedrich, A. Weinbrecht, E. Schreier, Seroprevalence and molecular epidemiology of enterovirus 71 in Germany. Arch. Virol. 154(7), 1139–1142 (2009)

    Article  CAS  PubMed  Google Scholar 

  11. B. Ortner, C.W. Huang, D. Schmid, I. Mutz, G. Wewalka, F. Allerberger, J.Y. Yang, H.P. Huemer, Epidemiology of enterovirus types causing neurological disease in Austria 1999–2007: detection of clusters of echovirus 30 and enterovirus 71 and analysis of prevalent genotypes. J. Med. Virol. 81(2), 317–324 (2009)

    Article  CAS  PubMed  Google Scholar 

  12. M. Ho, Enterovirus 71: the virus, its infections and outbreaks. J. Microbiol. Immunol. Infect. 33(4), 205–216 (2000)

    CAS  PubMed  Google Scholar 

  13. K. Mizuta, C. Abiko, T. Murata, Y. Matsuzaki, T. Itagaki, K. Sanjoh, M. Sakamoto, S. Hongo, S. Murayama, K. Hayasaka, Frequent importation of enterovirus 71 from surrounding countries into the local community of Yamagata, Japan, between 1998 and 2003. J. Clin. Microbiol. 43, 6171–6175 (2005)

    Article  CAS  PubMed  Google Scholar 

  14. P.V. Tu, N.T. Thao, D. Perera, T.K. Huu, N.T. Tien, T.C. Thuong, O.M. How, M.J. Cardosa, P.C. McMinn, Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005. Emerg. Infect. Dis. 11, 1733–1741 (2007)

    Google Scholar 

  15. K.H. Lin, K.P. Hwang, G.M. Ke, C.F. Wang, L.Y. Ke, Y.T. Hsu, Y.C. Tung, P.Y. Chu, B.H. Chen, H.L. Chen, C.L. Kao, J.R. Wang, H.L. Eng, S.Y. Wang, L.C. Hsu, H.Y. Chen, Evolution of EV71 genogroup in Taiwan from 1998 to 2005: an emerging of subgenogroup C4 of EV71. J. Med. Virol. 78(2), 254–262 (2006)

    Article  CAS  PubMed  Google Scholar 

  16. D. Perera, M.A. Yusof, Y. Podin, M.H. Ooi, N.T. Thao, K.K. Wong, A. Zaki, K.B. Chua, Y.A. Malik, P.V. Tu, N.T. Tien, P. Puthavathana, P.C. McMinn, M.J. Cardosa, Molecular phylogeny of modern coxsackievirus A16. Arch. Virol. 152(6), 1201–1208 (2007)

    Article  CAS  PubMed  Google Scholar 

  17. C. Savolainen, T. Hovi, M.N. Mulders, Molecular epidemiology of echovirus 30 in Europe: succession of dominant sublineages within a single major genotype. Arch. Virol. 146(3), 521–537 (2001)

    Article  CAS  PubMed  Google Scholar 

  18. W.A. Nix, M.S. Oberste, M.A. Pallansch, Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens. J. Clin. Microbiol. 44, 2698–2704 (2006)

    Article  CAS  PubMed  Google Scholar 

  19. A. Szendrõi, M. El-Sageyer, M. Takács, I. Mezey, G. Berencsi, Nucleotide sequences and mutations of the 5′-nontranslated region (5′-NTR) of natural isolates of an epidemic echovirus 11′ (prime). Arch. Virol. 145, 2575–2600 (2000)

    Article  PubMed  Google Scholar 

  20. E. Witsǿ, G. Palacios, K.S. Rønningen, O. Cinek, D. Janowitz, M. Rewers, B. Grinde, W.I. Lipkin, Asymptomatic circulation of HEV71 in Norway. Virus Res. 123, 19–29 (2007)

    Article  PubMed  CAS  Google Scholar 

  21. F. Yang, L. Ren, Z. Xiong, J. Li, Y. Xiao, R. Zhao, Y. He, G. Bu, S. Zhou, J. Wang, J. Qi, Enterovirus 71 outbreak in the People’s Republic of China in 2008. J. Clin. Microbiol. 47(7), 2351–2352 (2009)

    Article  PubMed  Google Scholar 

  22. S.R. Shih, M.S. Ho, K.H. Lin, S.L. Wu, Y.T. Chen, C.N. Wu, T.Y. Lin, L.Y. Chang, K.C. Tsao, H.C. Ning, P.Y. Chang, S.M. Jung, C. Hsueh, K.S. Chang, Genetic analysis of enterovirus 71 isolated from fatal and non-fatal cases of hand, foot and mouth disease during an epidemic in Taiwan, 1998. Virus Res. 68(2), 127–136 (2000)

    Article  CAS  PubMed  Google Scholar 

  23. P.C. McMinn, An overview of the evolution of enterovirus 71 and its clinical and public health significance. FEMS Microbiol. Rev. 26(1), 91–107 (2002)

    Article  CAS  PubMed  Google Scholar 

  24. M.J. Ferson, S.M. Bell, Outbreak of coxsackievirus A16 hand, foot, and mouth disease in a child day-care center. Am. J. Public Health 1, 1675–1676 (1991). 110

    Article  Google Scholar 

  25. J.L. Bailly, A. Mirand, C. Henquell, C. Archimbaud, M. Chambon, F. Charbonné, O. Traoré, H. Peigue-Lafeuill, Phylogeography of circulating populations of human echovirus 30 over 50 years:nucleotide polymorphism and signature of purifying selection in the VP1 capsid protein gene. Infect. Genet. Evol. 9(4), 699–708 (2009)

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful for support obtained in the frame of RiViGene Project (Genomic inventory, forensic markers, and assessment of potential therapeutic and vaccine targets for viruses relevant in biological crime and terrorism; Contract no. SSPE-CT-2005-022639). We would like to thank Dr. Alan Nix for helpful discussions, and for kindly sharing the protocol on enterovirus typing, during the training of B. K. at the Division of Viral Diseases, National Center for Immunisation and Respiratory Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA. Excellent technical assistance of Mrs. Anna Marchut, Mrs. Mária Török Kozmáné and Mrs. Ágota Pus Weller is very much appreciated.

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  1. Division of Virology, National Center for Epidemiology, Gyáli út 2-6, Budapest, 1097, Hungary

    Beatrix Kapusinszky, Katalin N. Szomor, Ágnes Farkas, Mária Takács & György Berencsi

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  1. Beatrix Kapusinszky
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  2. Katalin N. Szomor
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  3. Ágnes Farkas
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Correspondence to Mária Takács.

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Kapusinszky, B., Szomor, K.N., Farkas, Á. et al. Detection of non-polio enteroviruses in Hungary 2000–2008 and molecular epidemiology of enterovirus 71, coxsackievirus A16, and echovirus 30. Virus Genes 40, 163–173 (2010). https://doi.org/10.1007/s11262-009-0440-4

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  • DOI: https://doi.org/10.1007/s11262-009-0440-4

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