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Genome sequence of Leucania seperata nucleopolyhedrovirus

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Abstract

The nucleotide sequence of the Leucania seperata (Ls) Nucleopolyhedrovirus (LsNPV) genome has been determined and analyzed. The circular dsDNA genome contains 168041 bp, making it the largest NPV sequenced to date. The genome has a G + C content of 48.6% and encodes 169 predicted open reading frames (ORFs), one unique repeat region, and eight homologous repeat regions that are divided into two groups. Of the genome, 82.8% encodes predicted ORFs including five dispersal ORFs that have a large overlaps (range in 149 ∼ 390 bp) with their adjacent ORFs, respectively such as expression factor 10, 11, 5, 2 (lef-10, lef-11, lef-5, lef-2), and telokin-like protein-20 (tlp-20); 4.4% is in repeat regions; the remaining 12.8% of the genome comprises nonrepeat intergenic regions. LsNPV encodes homologues of 133 ORFs identified previously in other baculoviruses. Other than 10 ‘baculovirus repeat ORFs’ (bro) and two ‘inhibitor of apoptosis’ (iap) genes, no duplicated ORFs were found. LsNPV lacks a homologue of the ubiquitin gene, which has been found in all fully sequenced baculoviruses. Iap3 and p49, two genes were proven to be inhibitors of apoptosis by experiment, and are found in the LsNPV genome. It is not found in other baculoviruses that two kinds of inhibitors of apoptosis present in a baculovirus genome.

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Acknowledgment

We are grateful to Beijing Genomic Institute (Hua Da Gene Company) for its contributions to the sequencing work.

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Authors and Affiliations

  1. Institute of Virology, Wuhan University, Wuhan, 430072, P.R. China

    Huazhong Xiao & Yipeng Qi

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  1. Huazhong Xiao
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  2. Yipeng Qi
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Correspondence to Huazhong Xiao or Yipeng Qi.

Additional information

The GenBank accession number of the LsNPV genome sequence is AY394490.

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Xiao, H., Qi, Y. Genome sequence of Leucania seperata nucleopolyhedrovirus. Virus Genes 35, 845–856 (2007). https://doi.org/10.1007/s11262-007-0106-z

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