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Bacteria from bovine clinical mastitis showed multiple drug resistance

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Abstract

Mastitis, which often manifests as udder infection in dairy animals, is of great concern as it affects public health and results in heavy economic losses to the dairy industry. A hospital-based cross-sectional study was conducted to determine the cultivable bacterial species associated with bovine clinical mastitis and their resistance patterns towards different antimicrobials. The milk samples from cows suffering from clinical mastitis during monsoon season were investigated. The prevalence of clinical mastitis was significantly high in Holstein–Friesian crossbred cows, followed by in Jersey crossbred, Red Kandhari and Deoni. Significantly high prevalence was observed during 4th to 6th months of lactation. A total of 110 bacterial isolates belonging to 14 different genera were isolated and identified. Aminoglycosides and quinolones were found to be the most effective antibiotics. Vancomycin resistant penicillinase producing Gram positive bacteria were demonstrated. Gram negative bacteria resistant to extended spectrum β lactamases, cephalosporins, tetracyclines, vancomycin and chloramphenicol as well as vancomycin resistant enterococci, multiple drug resistant (MDR) gram negative rods, MDR Pseudomonas and MDR Acinetobacter were found. Widespread resistance of Streptococcus uberis towards cephalosporins was documented. Variable MDR patterns were recorded within a single species. MDR transfer from non-pathogens to emerging foodborne and established mastitis pathogens could be a potential problem to the dairy industry as well as to public health.

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Authors and Affiliations

  1. College of Veterinary and Animal Sciences, Udgir, Maharashtra, India

    Sudhakar P. Awandkar, Mahesh B. Kulkarni & Narendra V. Khode

  2. Maharashtra Animal and Fishery Sciences University, Nagpur, Maharashtra, India

    Sudhakar P. Awandkar, Mahesh B. Kulkarni & Narendra V. Khode

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  1. Sudhakar P. Awandkar
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  2. Mahesh B. Kulkarni
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  3. Narendra V. Khode
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Contributions

SPA contributed in conception, design and implementation of the work, reviewed and edited the initial draft of the manuscript. MBK provided technical and laboratory support in sample collection and processing, wrote the initial draft of the manuscript. NVK contributed in statistical analysis.

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Correspondence to Sudhakar P. Awandkar.

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Awandkar, S.P., Kulkarni, M.B. & Khode, N.V. Bacteria from bovine clinical mastitis showed multiple drug resistance. Vet Res Commun 46, 147–158 (2022). https://doi.org/10.1007/s11259-021-09838-8

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