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Peroral Application of Water-soluble Derivative of Propolis (WSDP) and Its Related Polyphenolic Compounds and Their Influence on Immunological and Antitumour Activity

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Abstract

Polyphenolic compounds are widely distributed in the plant kingdom and display a variety of biological activities, including chemoprevention and growth inhibition of tumours. Propolis contains a conglomerate of polyphenolic compounds. We investigated the effect of propolis and polyphenolic compounds, components of propolis, on the growth and metastatic potential of a transplantable mammary carcinoma (MCa) of the mouse. Metastases in the lung were generated by 2 x 10 tumour cells injected intravenously (i.v.). A water-soluble derivative of propolis (WSDP) and the polyphenolic compounds (caffeic acid (CA) and caffeic acid phenethyl ester (CAPE)) were given to mice perorally before or after tumour cell inoculation. WSDP, CA and CAPE reduced the number of metastases in the lung. This implies that the antitumour activities of the compounds used in these studies are mostly related to the immunomodulatory properties of the compounds, their cytotoxicity to tumour cells, and their ability to induce apoptosis and/or necrosis.

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Abbreviations

CA:

caffeic acid

CAPE:

caffeic acid phenethyl ester

CD:

cluster differentiation

Con A:

Concanavalin A

FITC:

fluorescein isothiocyanate

LAF:

lymphocyte activation factor

LPS:

lipopoly-saccharide

MCa:

mammary carcinoma

NO:

nitric oxid

PBS:

phosphate-buffered saline

PE:

phycoerythrin

PFC:

plaque-forming cell

PHA:

phytohaemagglutinin

PI:

propidium iodide

PWM:

pokeweed mitogen

SRBC:

sheep red blood cells

WSDP:

water-soluble derivative of proplis

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Authors and Affiliations

  1. Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000, Zagreb, Croatia

    N. Oršolic & I. Bašić

  2. Department of Biology, Veterinary Faculty, University of Zagreb, Zagreb, Croatia

    L. Šver

  3. Croatian Veterinary Institute, Zagreb, Croatia

    S. Terzić

Authors
  1. N. Oršolic
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  2. L. Šver
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  3. S. Terzić
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  4. I. Bašić
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Correspondence to N. Oršolic.

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Oršolic, N., Šver, L., Terzić, S. et al. Peroral Application of Water-soluble Derivative of Propolis (WSDP) and Its Related Polyphenolic Compounds and Their Influence on Immunological and Antitumour Activity. Vet Res Commun 29, 575–593 (2005). https://doi.org/10.1007/s11259-005-3303-z

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