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Excitatory and inhibitory effects of stimulation of sacral dorsal root ganglion on bladder reflex in cats

  • Urology - Original Paper
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Abstract

Purpose

To explore the effects of electrical stimulation of the sacral dorsal root ganglion (DRG) on bladder reflexes in α-chloralose-anesthetized cats.

Methods

Bladder activity was recorded under isovolumetric conditions. A pair of hook electrodes was placed in the right S1 and S2 DRGs of 12 adult male cats, which were stimulated over a range of frequencies (0.25–30 Hz) and at threshold intensity.

Results

Stimulation of S1 and S2 DRGs inhibited or evoked bladder contractions under isovolumetric conditions depending on the frequency of stimulation in nine cats. Stimulation at low frequencies (3–7 Hz on S1 or S2 DRG) significantly inhibited isovolumetric rhythmic bladder contractions, while excitatory effects were observed at two frequency ranges, including lower frequencies (0.25–1.5 Hz on S1 DRG and 0.25–1.25 Hz on S2 DRG) and middle frequencies (15–30 Hz on S1 and S2 DRGs).

Conclusions

These results suggest that the sacral DRG might be a potential valuable target for electrical stimulation in the treatment of bladder dysfunction.

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References

  1. Yoshimura N, de Groat WC (1997) Neural control of the lower urinary tract. Int J Urol 4:111–125

    Article  CAS  Google Scholar 

  2. Zhang F, Zhao S, Shen B, Wang J, Nelson DE, Roppolo JR, de Groat WC, Tai C (2013) Neural pathways involved in sacral neuromodulation of reflex bladder activity in cats. Am J Physiol Renal Physiol 304:710–717

    Article  Google Scholar 

  3. Yoo PB, Woock JP, Grill WM (2008) Bladder activation by selective stimulation of pudendal nerve afferents in the cat. Exp Neurol 212:218–225

    Article  Google Scholar 

  4. Fjorback MV, Rijkhoff N, Petersen T, Nohr M, Sinkjaer T (2006) Event driven electrical stimulation of the dorsal penile/clitoral nerve for management of neurogenic detrusor overactivity in multiple sclerosis. Neurourol Urodyn 25:349–355

    Article  Google Scholar 

  5. Woock JP, Yoo PB, Grill WM (2008) Activation and inhibition of the micturition reflex by penile afferents in the cat. Am J Physiol Regul Integr Comp Physiol 294:1880–1889

    Article  Google Scholar 

  6. Boggs JW, Wenzel BJ, Gustafson KJ, Grill WM (2006) Bladder emptying by intermittent electrical stimulation of the pudendal nerve. J Neural Eng 3:43–51

    Article  Google Scholar 

  7. Tai C, Wang J, Wang X, de Groat WC, Roppolo JR (2007) Bladder inhibition or voiding induced by pudendal nerve stimulation in chronic spinal cord injured cats. Neurourol Urodyn 26:570–577

    Article  Google Scholar 

  8. Creed KE, Tulloch AG (1978) The effect of pelvic nerve stimulation and some drugs on the urethra and bladder of the dog. Br J Urol 50:398–405

    Article  CAS  Google Scholar 

  9. Langdale CL, Hokanson JA, Sridhar A, Grill WM (2017) Stimulation of the pelvic nerve increases bladder capacity in the prostaglandin E2 rat model of overactive bladder. Am J Physiol Renal Physiol 313:657–665

    Article  Google Scholar 

  10. Dalmose AL, Rijkhoff NJ, Andersen IS, Stefania D, Jørgensen TM, Djurhuus JC (2002) Bladder and urethral responses to pelvic nerve stimulation in the pig. Scand J Urol Nephrol 210:34–45

    Article  Google Scholar 

  11. Bruns TM, Weber DJ, Gaunt A (2015) Microstimulation of afferents in the sacral dorsal root ganglia can evoke reflex bladder activity. Neurourol Urodyn 34:65–71

    Article  Google Scholar 

  12. Snellings AE, Grill WM (2012) Effects of stimulation site and stimulation parameters on bladder inhibition by electrical nerve stimulation. BJU Int 110:136–143

    Article  Google Scholar 

  13. Bremer N, Ruby J, Weyker PD, Webb CA (2016) Neuromodulation: a focus on dorsal root ganglion stimulation. Pain Manag 6:205–209

    Article  Google Scholar 

  14. Liem L (2016) Stimulation of the dorsal root ganglion. Prog Neurol Surg 29:213–224

    Article  Google Scholar 

  15. Koopmeiners AS, Mueller S, Kramer J, Hogan QH (2013) Effect of electrical field stimulation on dorsal root ganglion neuronal function. Neuromodulation 16:304–311

    Article  Google Scholar 

  16. de Groat WC (1990) Central neural control of the lower urinary tract. Ciba Found Symp 151:27–44

    PubMed  Google Scholar 

  17. Fowler CJ, Griffiths D, de Groat WC (2008) The neural control of micturition. Nat Rev Neurosci 9:453

    Article  CAS  Google Scholar 

  18. Lu Y, Inokuchi H, Tanaka E, Li JS, Higashi H (2000) A spinal cord slice preparation for analyzing synaptic responses to stimulation of pelvic and pudendal nerves in mature rats. J Neurosci Methods 100:71–78

    Article  CAS  Google Scholar 

  19. de Groat WC, Booth AM, Milne RJ, Roppolo JR (1982) Parasympathetic preganglionic neurons in the sacral spinal cord. J Auton Nerv Syst 5:23–43

    Article  Google Scholar 

  20. Angel MJ, Fyda D, Mccrea DA, Shefchyk SJ (1994) Primary afferent depolarization of cat pudendal afferents during micturition and segmental afferent stimulation. J Physiol 479:451–461

    Article  Google Scholar 

  21. Xiao Z, Reese J, Schwen Z, Shen B, Wang J, Roppolo JR, de Groat WC, Tai C (2014) Role of spinal GABAA receptors in pudendal inhibition of nociceptive and nonnociceptive bladder reflexes in cats. Am J Physiol Renal Physiol 306:781–789

    Article  Google Scholar 

  22. Chen ML, Shen B, Wang J, Liu H, Roppolo JR, de Groat WC (2010) Influence of naloxone on inhibitory pudendal-to-bladder reflex in cats. Exp Neurol 224:282–291

    Article  CAS  Google Scholar 

  23. Peters KM, Feber KM, Bennett RC (2007) A prospective, single-blind, randomized crossover trial of sacral vs pudendal nerve stimulation for interstitial cystitis. BJU Int 100:835–839

    Article  Google Scholar 

  24. Goldman HB, Amundsen CL, Mangel J, Grill J, Bennett M, Gustafson KJ, Grill WM (2008) Dorsal genital nerve stimulation for the treatment of overactive bladder symptoms. Neurourol Urodyn 27:499–503

    Article  Google Scholar 

  25. Boggs JW, Wenzel BJ, Gustafson KJ, Grill WM (2006) Frequency-dependent selection of reflexes by pudendal afferents in the cat. J Physiol 577:115–126

    Article  CAS  Google Scholar 

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Funding

This study was funded by Research Projects of China Rehabilitation Research Center (2017ZX-31 and 2016CZ-1) and National Natural Science Foundation of China (Grand No. 81570688).

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Authors and Affiliations

  1. Department of Urology, China Rehabilitation Research Centre, Rehabilitation School of Capital Medical University, No 10. Jiaomen Beilu, Fengtai District, Beijing, 100068, China

    Zhaoxia Wang, Han Deng, Limin Liao, Tianji Lu & Xing Li

  2. Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China

    Zhaoxia Wang, Han Deng, Limin Liao, Tianji Lu & Xing Li

  3. Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China

    Zhaoxia Wang, Han Deng, Limin Liao, Tianji Lu & Xing Li

Authors
  1. Zhaoxia Wang
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  2. Han Deng
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  3. Limin Liao
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  4. Tianji Lu
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  5. Xing Li
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Corresponding author

Correspondence to Limin Liao.

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Conflict of interest

No potential conflict of interest relevant to this article was reported.

Ethical approval

All animal experiments were performed ethically in accordance with Capital Medical University guidelines for animal care and use.

Research involving human and animal participants

Human subjects were not used in this study.

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Wang, Z., Deng, H., Liao, L. et al. Excitatory and inhibitory effects of stimulation of sacral dorsal root ganglion on bladder reflex in cats. Int Urol Nephrol 50, 2179–2186 (2018). https://doi.org/10.1007/s11255-018-2004-9

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  • DOI: https://doi.org/10.1007/s11255-018-2004-9

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