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Augmenter of liver regeneration attenuates inflammation of renal ischemia/reperfusion injury through the NF-kappa B pathway in rats

  • Nephrology - Original Paper
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Abstract

Purpose

The effects of augmenter of liver regeneration (ALR) on the acute kidney injury (AKI) rats were investigated by measuring the inflammatory response associated with transcription factor nuclear factory (NF-κB) pathway.

Methods

The model of AKI rats was established by occluded the renal pedicles for 60 min and then released. After that, animals were treated with ALR (100 or 200 μg/kg). All rats were killed at different time points (24, 48, 72 h). Renal function and kidney histological changes were measured. The apoptosis of tubular cells was evaluated by TdT-mediated dUTP nick end labeling assay. Cytokines and chemokines were assessed by immunohistochemistry, enzyme-linked immunosorbent assay and real-time polymerase chain reaction (RT-PCR). The NF-κB p65 protein was analyzed by immunohistochemistry and RT-PCR, respectively.

Results

Ischemia reperfusion induced tubular cells necrosis and apoptosis, and ALR can significantly reduce this damages. The productions of MCP-1, IL-1β and IL-6 were lower in the group of ALR treatment, especially in the high-dose group. The inflammatory infiltrates were lower in the rats with administration of ALR. ALR mediated the level of cytokines and chemokines through inhibited the activation of NF-κB.

Conclusion

ALR can improve renal function and inhibit the expression of inflammatory factors. This protects against renal ischemia reperfusion injury, which may be associated with preventing NF-κB activation in rats.

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Acknowledgments

This work is supported by grants from the National Natural Scientific Foundation of China (No. 30971364) and the Applied Basic Research Program of the Public Health Bureau Foundation of Chongqing Province (Nos. 2010-2-126).

Conflict of interest

We declare that we have no conflicts of interest.

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Authors and Affiliations

  1. Department of Nephrology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China

    Ruyu Yan, Ying Li, Ling Zhang & Ning Xia

  2. Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China

    Qi Liu, Hang Sun & Hui Guo

Authors
  1. Ruyu Yan
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  2. Ying Li
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  3. Ling Zhang
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  4. Ning Xia
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  5. Qi Liu
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  6. Hang Sun
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  7. Hui Guo
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Corresponding author

Correspondence to Ling Zhang.

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Yan, R., Li, Y., Zhang, L. et al. Augmenter of liver regeneration attenuates inflammation of renal ischemia/reperfusion injury through the NF-kappa B pathway in rats. Int Urol Nephrol 47, 861–868 (2015). https://doi.org/10.1007/s11255-015-0954-8

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  • DOI: https://doi.org/10.1007/s11255-015-0954-8

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