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GSTO1*C/GSTO2*G haplotype is associated with risk of transitional cell carcinoma of urinary bladder

  • Urology - Original Paper
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Abstract

Purpose

To clarify the role of genetic polymorphisms of GSTO1 (rs4925) and GSTO2 (rs156697) in individual susceptibility to urinary bladder cancer.

Methods

Case–control study consisting of 187 patients with histologically confirmed transitional cell carcinoma (TCC) of urinary bladder and 140 age- and gender-matched cancer-free controls was carried out. Genotyping of GSTO1 and GSTO2 was performed by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP).

Results

We found that carriers of mutant GSTO2*G/G genotype were at increased risk of the development of TCC (OR 2.6, 95 % CI 1.2–5.8, p = 0.041), while GSTO1 rs4925 polymorphism was not significantly associated with TCC risk (p = 0.450). According to smoking status, smokers with GSTO2*G/G genotype had significantly higher risk of TCC of urinary bladder (OR 4.3, 95 % CI 1.6–11.2, p = 0.003) compared to wild-type carriers with no smoking history. We further analyzed the effects of GSTO1/GSTO2 haplotypes on TCC risk, based on the linkage disequilibrium found for GSTO1 (rs4925) and GSTO2 (rs156697) (D′ = 0.309, p = 0.001). The study subjects with GSTO1*C/GSTO2*G (GSTO1 wild-type/GSTO2 mutant) haplotype were at the highest risk of the development of transitional cell carcinoma of urinary bladder (OR 2.8, 95 % CI 1.5–5.2, p = 0.002).

Conclusions

Our results indicate that GSTO1*C/GSTO2*G haplotype is associated with increased risk of TCC. The modifying effect of GSTO2*G/G genotype on individual susceptibility to TCC is more pronounced, when associated with smoking.

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Acknowledgments

This work was supported by the Grant 175052 from the Serbian Ministry of Education, Science and Technological Developmental.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Authors and Affiliations

  1. Faculty of Medicine, Institute of Medical and Clinical Biochemistry, University of Belgrade, Pasterova 2, 11000, Belgrade, Serbia

    Tatjana Djukic, Tatjana Simic, Tanja Radic, Marija Matic, Marija Pljesa-Ercegovac, Sonja Suvakov, Vesna Coric & Ana Savic-Radojevic

  2. Faculty of Medicine, Institute of Epidemiology, University of Belgrade, Visegradska 26a, 11000, Belgrade, Serbia

    Tatjana Pekmezovic

  3. Faculty of Medicine, Institute of Biology and Human Genetics, University of Belgrade, Visegradska 26, 11000, Belgrade, Serbia

    Ivana Novakovic

  4. Clinic of Urology, Faculty of Medicine, Clinical Centre of Serbia, University of Belgrade, Resavska 51, 11000, Belgrade, Serbia

    Dejan Dragicevic

Authors
  1. Tatjana Djukic
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  2. Tatjana Simic
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  3. Tanja Radic
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  4. Marija Matic
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  5. Marija Pljesa-Ercegovac
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  7. Vesna Coric
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  10. Dejan Dragicevic
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  11. Ana Savic-Radojevic
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Correspondence to Ana Savic-Radojevic.

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Djukic, T., Simic, T., Radic, T. et al. GSTO1*C/GSTO2*G haplotype is associated with risk of transitional cell carcinoma of urinary bladder. Int Urol Nephrol 47, 625–630 (2015). https://doi.org/10.1007/s11255-015-0933-0

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  • DOI: https://doi.org/10.1007/s11255-015-0933-0

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