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Roles of MAPKAPK-2 and HSP27 in the reduction of renal ischemia–reperfusion injury by ischemic postconditioning in rats

  • Nephrology - Original Paper
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Abstract

Purpose

Ischemic postconditioning is a procedure during which intermittent reperfusions are performed in the early phase of reperfusion to protect organs from ischemia/reperfusion injury. And in this study, we mainly investigated the injury-alleviative role of mitogen-activated protein kinase-activating protein kinase-2 (MAPKAPK-2) and heat shock protein 27 (HSP27) in renal ischemic reperfusion injury during the procedure of ischemic postconditioning.

Methods

Sprague-Dawley rats were randomly divided into four groups. The injury models were prepared by clipping the left renal pedicle of rats after ligating the right renal pedicle for 60 min. In the ischemic postconditioning group, sequential reperfusions were done for 10 s and another ischemia for 10 s for six cycles after kidney ischemia for 60 min. In addition, the specific inhibitor SB203580 was injected through caudal vein before ischemia. Serum creatinine, blood urea nitrogen and the expression of HSP27 and MAPKAPK-2 were detected 1, 3, 6 and 24 h later after reperfusion. Furthermore, phosphorylation of HSP27 and MAPKAPK-2 protein contents, histological changes and apoptosis were compared 24 h later after reperfusion.

Results

Our data showed that ischemic postconditioning attenuated the renal dysfunction and cell apoptosis induced by I/R and increased phosphorylation of MAPKAPK-2 and HSP27. The results indicated that ischemic postconditioning decreased apoptosis and improved renal function.

Conclusions

Taken together, it is suggested that the renal protective effect may be related to the levels of HSP27 and MAPKAPK-2 activation.

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Acknowledgments

This study was supported by a grant from the Bureau of Science and Technology, Xuzhou, China (XF10C072). We declare that no conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication.

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Authors and Affiliations

  1. Department of Pharmacology, School of Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou, 221004, Jiangsu Province, China

    Anzhou Xia, Na Li, Zhi Xue, Jieyun Xia, Tiantian Wei & Jing Cao

  2. Department of Gastrointestinal Surgery, Xuzhou Central Hospital, 199 Jiefang North Road, Xuzhou, 221009, Jiangsu Province, China

    Yong Li

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  1. Anzhou Xia
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  2. Yong Li
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  3. Na Li
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Correspondence to Anzhou Xia.

Additional information

Anzhou Xia, Yong Li, Na Li and Zhi Xue have contributed equally to this work.

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Xia, A., Li, Y., Li, N. et al. Roles of MAPKAPK-2 and HSP27 in the reduction of renal ischemia–reperfusion injury by ischemic postconditioning in rats. Int Urol Nephrol 46, 1455–1464 (2014). https://doi.org/10.1007/s11255-014-0748-4

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  • DOI: https://doi.org/10.1007/s11255-014-0748-4

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