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Bladder function in 17β-estradiol-induced nonbacterial prostatitis model in Wister rat

  • Urology – Original Paper
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Abstract

Objectives

To investigate bladder function in a model of nonbacterial prostatitis (NBP) induced in castrated rats by 17β-estradiol injection.

Methods

Ten-month-old male Wistar rats were divided into two groups, sham and NBP (both N = 8). NBP was induced by castration followed by daily subcutaneous injection of 17β-estradiol for 30 days. On the 31st day after surgery, we investigated (1) voiding behavior, (2) bladder blood flow (BBF), (3) prostate and bladder weight, and proinflammatory cytokines (TNF-α and CXCL1) levels and (4) bladder contractile responses to electrical field stimulation (EFS), carbachol and KCl.

Results

(1) Voiding behavior (average micturition volume, total urine volume and number of micturitions) and (2) BBF were not significantly different between the sham and NBP groups. (3) NBP led to a significant decrease in prostatic weight and increase in proinflammatory cytokine levels in the prostate, but NBP did not cause a significant change in bladder weight or proinflammatory cytokine levels in the bladder. (4) Bladder contractile forces in response to EFS, carbachol and KCl were not significantly affected by NBP.

Conclusions

In this rat model, NBP did not cause a significant change in the level of proinflammatory cytokines in the bladder and affect bladder function.

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References

  1. Nickel JC, Roehrborn CG, O’Leary MP, Bostwick DG, Somerville MC, Rittmaster RS (2008) The relationship between prostate inflammation and lower urinary tract symptoms: examination of baseline data from the REDUCE trial. Eur Urol 54:1379–1384

    Article  PubMed  Google Scholar 

  2. Krieger JN, Nyberg L Jr, Nickel JC (1999) NIH consensus definition and classification of prostatitis. JAMA 282:236–237

    Article  PubMed  CAS  Google Scholar 

  3. Gonzalez RR, Te AE (2006) Is there a role for urodynamics in chronic nonbacterial prostatitis? Curr Urol Rep 7:335–338

    Article  PubMed  Google Scholar 

  4. Bernoulli J, Yatkin E, Talvitie EM, Santti R, Streng T (2007) Urodynamic changes in a Noble rat model for nonbacterial prostatic inflammation. Prostate 67:888–899

    Article  PubMed  CAS  Google Scholar 

  5. Funahashi Y, Takahashi R, Gotoh M, Yoshimura N (2012) Bladder overactivity induced by prostate-to-bladder afferent cross-sensitization in rats with prostatic inflammation. J Urol 187:e442. In: Abstract no. 1090. Poster presented at the annual meeting of the American Urological Association, May 21, 2012, Atlanta, GA, USA

  6. Naslund MJ, Strandberg JD, Coffey DS (1988) The role of androgens and estrogens in the pathogenesis of experimental nonbacterial prostatitis. J Urol 140:1049–1053

    PubMed  CAS  Google Scholar 

  7. Kawai Y, Oka M, Kyotani J, Oyama T, Matsumoto S, Kakizaki H (2012) Effect of the phytotherapeutic agent eviprostat on the bladder in a rat model of bladder overdistension/emptying. Neurourol Urodyn. doi:10.1002/nau.22350

    Google Scholar 

  8. Oka M, Fukui T, Ueda M, Tagaya M, Oyama T, Tanaka M (2009) Suppression of bladder oxidative stress and inflammation by a phytotherapeutic agent in a rat model of partial bladder outlet obstruction. J Urol 182:382–390

    Article  PubMed  CAS  Google Scholar 

  9. Oka M, Ueda M, Oyama T, Kyotani J, Tanaka M (2009) Effect of the phytotherapeutic agent Eviprostat® on 17β-estradiol-induced nonbacterial inflammation in the rat prostate. Prostate 69(13):1404–1410

    Article  PubMed  CAS  Google Scholar 

  10. Sugimoto M, Oka M, Tsunemori H, Yamashita M, Kakehi Y (2011) Effect of a phytotherapeutic agent, Eviprostat®, on prostatic and urinary cytokines/chemokines in a rat model of nonbacterial prostatitis. Prostate 71:438–444

    Article  PubMed  CAS  Google Scholar 

  11. Shabsigh A, Chang DT, Heitjan DF, Kiss A, Olsson CA, Puchner PJ, Buttyan R (1998) Rapid reduction in blood flow to the rat ventral prostate gland after castration: preliminary evidence that androgens influence prostate size by regulating blood flow to the prostate gland and prostatic endothelial cell survival. Prostate 36:201–206

    Article  PubMed  CAS  Google Scholar 

  12. Robinette CL (1988) Sex-hormone-induced inflammation and fibromuscular proliferation in the rat lateral prostate. Prostate 12:271–286

    Article  PubMed  CAS  Google Scholar 

  13. Kamijo T, Sato S, Kitamura T (2001) Effect of cernitin pollen-extract on experimental nonbacterial prostatitis in rats. Prostate 49:122–131

    Article  PubMed  CAS  Google Scholar 

  14. Ishigooka M, Zermann DH, Doggweiler R, Schmidt RA (2000) Similarity of distributions of spinal c-Fos and plasma extravasation after acute chemical irritation of the bladder and the prostate. J Urol 164:1751–1756

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Renal and Urologic Surgery, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan

    Seiji Matsumoto, Kazumi Hashizume, Naoki Wada, Jun-ichi Hori, Gaku Tamaki, Masafumi Kita, Tatsuya Iwata & Hidehiro Kakizaki

  2. Research Laboratories, Nippon Shinyaku Co., Ltd., 14, Nishinosho-monguchi-cho, Kisshoin, Minami-ku, Kyoto, 601-8550, Japan

    Yuko Kawai, Michiko Oka & Tatsuya Oyama

Authors
  1. Seiji Matsumoto
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  2. Yuko Kawai
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  3. Michiko Oka
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  4. Tatsuya Oyama
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  5. Kazumi Hashizume
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  6. Naoki Wada
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  7. Jun-ichi Hori
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  8. Gaku Tamaki
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  9. Masafumi Kita
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  10. Tatsuya Iwata
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  11. Hidehiro Kakizaki
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Corresponding author

Correspondence to Seiji Matsumoto.

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Matsumoto, S., Kawai, Y., Oka, M. et al. Bladder function in 17β-estradiol-induced nonbacterial prostatitis model in Wister rat. Int Urol Nephrol 45, 749–754 (2013). https://doi.org/10.1007/s11255-013-0442-y

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  • DOI: https://doi.org/10.1007/s11255-013-0442-y

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