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Ginkgo biloba (EGB 761) affects apoptosis and nitric-oxide synthases in testicular torsion: an experimental study

  • Urology - Original Paper
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Abstract

Introduction

We investigated the effect of ginkgo biloba on germ cell apoptosis and also on expressions of endothelial (eNOS) and inducible (iNOS) nitric oxide synthases after testicular torsion.

Materials and methods

Thirty-two Wistar albino rats were randomly assigned into four groups. Torsion/detorsion (T/D) was performed on the rats in group 1, group 2 received ginkgo biloba for a month before T/D, group 3 received only gingko biloba for a month, and group 4 was the sham group. Left testicular torsion was created in group 1 and group 2, and the testes were untwisted and replaced in the scrotum for reperfusion. No procedure was applied to group 3, and after 1 month, testes were removed in all groups.

Results

Mean apoptotic cell, eNOS, and iNOS were increased in group 1. Group 2 showed significantly decreased apoptotic cells, eNOS, and iNOS in testes compared to group 1 (P < 0.05). The rats in group 3 had significantly decreased apoptotic cell, eNOS, and iNOS values, like the sham group (P < 0.05), and this group provided basal values.

Conclusions

Ginkgo biloba, as a free radical scavenger, seems to have a protective role against apoptosis in testicular ischemia reperfusion injury.

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Authors and Affiliations

  1. Department of Second Urology Clinic, Ministry of Health Ankara Training and Research Hospital, Ankara, Turkey

    Turgay Akgül, Ersagun Karagüzel, Ali Ayyıldız & Cankon Germiyanoğlu

  2. Department of Biochemistry and Clinical Biochemistry, Ministry of Health Ankara Training and Research Hospital, Ankara, Turkey

    Hatice Sürer

  3. Department of Anesthesiology, Ministry of Health Ankara Training and Research Hospital, Ankara, Turkey

    Hatice Yağmurdur

  4. Department of Pathology, Ministry of Health Ankara Training and Research Hospital, Ankara, Turkey

    Hüseyin Üstün

Authors
  1. Turgay Akgül
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  2. Ersagun Karagüzel
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  3. Hatice Sürer
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  4. Hatice Yağmurdur
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  6. Hüseyin Üstün
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Correspondence to Turgay Akgül.

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Akgül, T., Karagüzel, E., Sürer, H. et al. Ginkgo biloba (EGB 761) affects apoptosis and nitric-oxide synthases in testicular torsion: an experimental study. Int Urol Nephrol 41, 531–536 (2009). https://doi.org/10.1007/s11255-008-9511-z

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  • DOI: https://doi.org/10.1007/s11255-008-9511-z

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