Summary
Reperfusion injury has been well documented in organs other than testis. An experimental study was conducted to investigate reperfusion injury in testes via the biochemical changes after unilateral testicular torsion and detorsion. As unilateral testicular torsion and varicocele have been shown to affect contralateral testicular blood flow, reperfusion injury was studied in both testes. Given that testicular blood flow does not return after 720° testicular torsion lasting more than 3 h, the present study was conducted after 1 and 2 h of 720° torsion. Adult male albino rats were divided into seven groups each containing ten rats. One group served to determine the basal values of biochemical parameters, two groups were subjected to 1 and 2 h of unilateral testicular torsion respectively, two groups were subjected to detorsion following 1 and 2 h of torison respectively, and two groups underwent sham operations as a control. Levels of lactic acid, hypoxanthine and lipid peroxidation products were determined in testicular tissues. Values of these three parameters obtained from the sham operation control groups did not differ significantly from basal values (P>0.05). All three parameters were increased significantly in both ipsilateral and contralateral testes after unilateral testicular torsion when compared with basal values (P<0.01 and P<0.05, respectively). Detorsion caused significant changes in lipid peroxidation products levels in ipsilateral but not in contralateral testes when compared with values obtained after torsion (P<0.01 and P>0.05, respectively). It is concluded that ipsilateral testicular torsion causes a decrease in perfusion not only in the ipsilateral but also in the contralateral testis. Additionally, detorsion following up to 2 h of 720° torsion causes reperfusion injury in ipsilateral but not in contralateral testis.
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Akgür FM, Kilinç K, Tanyel FC, Büyükpamukçu N, Hiçsönmez A (in press) Ipsilateral and contralateral biochemical changes after unilateral testicular torsion and detorsion. Urology
Akgür FM, Kilinç K, Aktuğ T (in press) Is ipsilateral testis mandatory for the contralateral biochemical changes after unilateral spermatic cord torsion? Urology
Bergh A, Damber JE, Marklund SE (1988) Morphologic changes induced by short term ischemia in rat testis are not affected by treatment with superoxide dismutase or catalase. J Androl 9:15
Cosentino MJ, Nishida M, Rabinowitz R, Cockett ATK (1986) Histopathology of prepubertal rats subjected to various durations of spermatic cord torsion. J Androl 7:23
Cosentino MJ, Nishida M, Rabinowitz R, Cockett ATK (1986) Histological changes occurring in contralateral testes of prepubertal rats subjected to various durations of spermatic cord torsion. J Urol 133:906
Feher J, Csomos G, Vereckei A (1987) Free radical reactions in medicine. Springer, Berlin Heidelberg New York
Goldstein M, Eid JF (1989) Elevation of intratesticular and scrotal skin surface temperature in man with varicocele. J Urol 142:743
Green KF, Turner TT, Howards SS (1985) Effects of varicocele after unilateral orchiectomy and sympathectomy. J Urol 134:378
Hurt GS, Howards SS, Turner TT (1987) The effects of unilateral varicocele are not mediated through the ipsilateral testis. J Androl 8:403
Jong JW, Goldstein S (1974) Changes in coronary venous inosine concentration and myocardial wall thickening during regional ischemia in the pig. Circ Res 35:111
Kagan VE (1988) Lipid peroxidation in biomembranes. CRC Press, Boca Raton, Fla.
Kaya M, Harrison RG (1975) An analysis of the effect of ischemia on testis ultrastructure. J Pathol 117:105
Kizilcan F, Bernay İ, Tanyel FC, Büyükpamukçu N, Hiçsönmez A (1992) Ipsilateral and contralateral testicular blood flows during unilateral testicular torsion by 133Xe clearance technique. Int Urol Nephrol 24:515
Olsson RA (1970) Changes in content of purine nucleoside in canine myocardium during coronary occlusion. Circ Res 26:301
Reilly PM, Schiller HJ, Bulkley GB (1992) Pharmacologic approach to tissue injury mediated by free radicals and other reactive oxygen metabolites. Am J Surg 161:488
Saugstad OD (1988) Hypoxanthine as an indicator of hypoxia: its role in health and disease through free radical production. Pediatr Res 23:143
Tanyel FC, Büyükpamukçu N, Hiçsönmez A (1989) Contralateral testicular blood flow during unilateral testicular torsion. Br J Urol 63:522
Tietz NW (1986) Determination of lactate in whole blood. In: Textbook of clinical chemistry. Saunders, Philadelphia
Turner TT (1985) Acute experimental testicular torsion: no effect on the contralateral testis. J Androl 6:65
Turner TT, Lopez TJ (1990) Testicular blood flow in peripubertal and older rats with unilateral experimental varicocele and investigation into the mechanism of the bilateral response to the unilateral lesion. J Urol 144:1018
Uchiama M, Mihara M (1977) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:271
Walsh TR, Rao PN, Makowka L, Starzl T (1990) Lipid peroxidation is a nonparenchymal cell event with reperfusion after prolonged liver ischemia. J Surg Res 49:18
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Akgür, F.M., Kilinç, K. & Aktuĝ, T. Reperfusion injury after detorsion of unilateral testicular torsion. Urol. Res. 21, 395–399 (1993). https://doi.org/10.1007/BF00300075
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DOI: https://doi.org/10.1007/BF00300075