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CYP1A2, CYP2D6, GSTM1, GSTP1, and GSTT1 gene polymorphisms in patients with bladder cancer in a Turkish population

  • Urology - Original Paper
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Abstract

Genetic differences in the metabolism of xenobiotics have recently been suggested as modifiers of individual susceptibility to bladder cancer (BC). The objective of this study was to investigate the relationship between bladder tumor and variants of cytochrome p450 1A2 (CYP1A2) 734 C → A, cytochrome p450 2D6 (CYP2D6) 1934 G → A, glutathione S-transferase M1, (GSTM1 null), glutathione S-transferase T1 (GSTT1 null), and glutathione S-transferase P1 (GSTP1) I105 V. We investigated the distribution of these polymorphisms in 135 BC patients and in 128 age and sex-matched cancer-free controls. The polymorphisms were analyzed using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay and the multiplex PCR method. Genotype and allele frequencies and their associations with BC risk, demographic factors, smoking status, and tumor stage were investigated. The prevalence of GSTT1 null genotype in cases was 23%, compared with 7% in the control group (OR = 3.94, 95% CI = 1.70–9.38, P = 0.001). There was no association between the studied polymorphisms of CYP1A2, CYP2D6, GSTM1, and GSTP1 genes and BC. There was an association between smoking status and BC. These data seem to indicate that GSTT1 gene polymorphism may be associated with BC in the Turkish population studied. Further studies will be needed to clarify the role of such variation in determining susceptibility to BC.

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Acknowledgements

This study was completely supported by Ondokuz Mayis University Research Fund (Project No. 402). We also thank Dr Cem Irkilata for providing some of the samples used in this study.

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Authors and Affiliations

  1. Department of Medical Biology and Genetics, Faculty of Medicine, Ondokuz Mayis University, 55139, Samsun, Turkey

    Ertan Altayli & Sezgin Gunes

  2. Department of Urology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Ali Faik Yilmaz

  3. Department of Urology, Faculty of Medicine, Gulhane Military Medical Academy, Ankara, Turkey

    Serdar Goktas

  4. Department of Biostatistics, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    Yuksel Bek

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  1. Ertan Altayli
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  2. Sezgin Gunes
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Correspondence to Sezgin Gunes.

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Altayli, E., Gunes, S., Yilmaz, A.F. et al. CYP1A2, CYP2D6, GSTM1, GSTP1, and GSTT1 gene polymorphisms in patients with bladder cancer in a Turkish population. Int Urol Nephrol 41, 259–266 (2009). https://doi.org/10.1007/s11255-008-9444-6

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