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Histogenesis of Human Renal Cell Carcinoma by using Electron Microscopy and Immunohistochemical Techniques

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Abstract

Electron microscopy and immunohistochemical techniques are powerful tools for the determination of tissue origin. Both techniques have been used in the current experiment for histogenesis of renal cell carcinoma. Fifty kidney tumors were subjected to immunohistochemical detection for intermediate filaments cytokeratin and vimentin, which are normally expressed in epithelial tissue and mesenchymal tissues, respectively. Twenty cases of the above were examined by electron microscopy for detection of ultrastructure features. From each kidney, two specimens were taken, one from the diseased area and another far from it to serve as a control. Immunohistochemical study revealed in cases of renal cell carcinoma, cytokeratin and vimentin were expressed alone in 44% of cases, and 40% of cases, respectively. Twelve percent of cases were coexpressed with both cytokeratin and vimentin. Electron microscopic study of diseased specimens revealed the expression of desmosomes which was observed in almost all tumor specimens. The expression of the vimentin in some cases either alone or with cytokeratin was interpreted as a change in the characters of some tumor cells which indicates the need for additional techniques in such cases to get the proper interpretation. The prevalence of the expression of cytokeratin and the persistence existence of desmosomes indicate the epithelial origin of the tumor. This data is very beneficial for determination of line of therapy and follow up of the patients. The results confirm the power of combined use of both immunohistochemistry and electron microscopy in the field of histogenesis.

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References

  1. GE Quinonez (1999) ArticleTitleDifferentiation, histogenesis and morphogenesis: their implications for tumor diagnosis Med Hypotheses 53 IssueID3 243–245 Occurrence Handle10.1054/mehy.1998.0753 Occurrence Handle10580531 Occurrence Handle1:STN:280:DC%2BD3c%2Fks1CktA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  2. RR Verani FG Silva (1988) ArticleTitleHistogenesis of the renal cysts in adult (autosomal dominant) polycystic kidney disease: a histochemical study Mod Pathol 1 IssueID6 457–463 Occurrence Handle3065782 Occurrence Handle1:STN:280:DyaL1M7ivVyhtQ%3D%3D

    PubMed  CAS  Google Scholar 

  3. Y Takeshima K Inai K Yoneda (1996) ArticleTitlePrimary carcinoid tumor of the kidney with special reference to its histogenesis Pathol Int 46 IssueID11 894–900 Occurrence Handle8970200 Occurrence Handle1:STN:280:DyaK2s7isFSqtA%3D%3D Occurrence Handle10.1111/j.1440-1827.1996.tb03564.x

    Article  PubMed  CAS  Google Scholar 

  4. M Sanchez-Carbayo ND Socci E Charytonowicz M Lu M Prystowsky G Childs C Cordon-Cardo (2002) ArticleTitleMolecular profiling of bladder cancer using cDNA microarrays: defining histogenesis and biological phenotypes Cancer Res 162 IssueID23 6973–6980

    Google Scholar 

  5. H Hentschel (1991) ArticleTitleDeveloping nephrons in adolescent dogfish, Scyliorhinus caniculus (L.), with reference to ultrastructure of early stages, histogenesis of the renal countercurrent system, and nephron segmentation in marine elasmobranchs Am J Anat Apr 190 IssueID4 309–333 Occurrence Handle1:STN:280:DyaK3M3otFKqtw%3D%3D

    CAS  Google Scholar 

  6. G Scala N Mirabella P Girolamo Particlede GV Pelagalli (2001) ArticleTitleMorphostructural changes of the kidney microvasculature during the prenatal period in Bubalus bubalis Ital J Anat Embryol 106 IssueID2 Suppl 1 229–236 Occurrence Handle11729960 Occurrence Handle1:STN:280:DC%2BD3MnotlCnsw%3D%3D

    PubMed  CAS  Google Scholar 

  7. F Satoh Y Tsutsumi S Yokoyama RY Osamura (2000) ArticleTitleComparative immunohistochemical analysis of developing kidneys, nephroblastomas and related tumors: considerations on their histogenesis Pathol Int 50 IssueID6 458–471 Occurrence Handle10.1046/j.1440-1827.2000.01070.x Occurrence Handle10886722 Occurrence Handle1:STN:280:DC%2BD3cvisFehug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  8. Y Hiasa M Kitamura S Nakaoka M Ohshima N Konishi Y Kitahori K Hirao Y Fukushima Y Tho I Hayashi (1995) ArticleTitleAntigen immunohistochemistry of renal cell adenomas in autopsy cases: relevance to histogenesis Oncology 52 IssueID2 97–105 Occurrence Handle7531836 Occurrence Handle1:STN:280:DyaK2M7lvVSitw%3D%3D Occurrence Handle10.1159/000227438

    Article  PubMed  CAS  Google Scholar 

  9. G Robinson (1982) Immunohistochemistry J Bancroft A Stevens (Eds) Theory and practice of Histochemical Techniques EditionNumber2 Churchill living stone Edinburgh-London and New York 406

    Google Scholar 

  10. AS Leong J Wright (1987) ArticleTitleThe contribution of immunhistochemical staining in tumour diagnosis Histopathology 11 1295–1299 Occurrence Handle3326815 Occurrence Handle1:STN:280:DyaL1c7lt1Ontw%3D%3D

    PubMed  CAS  Google Scholar 

  11. D Cooper A Schermer TT Sun (1985) ArticleTitleBiology of the disease: classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations Lab Invest 52 243–248 Occurrence Handle2579289 Occurrence Handle1:CAS:528:DyaL2MXitF2hu7g%3D

    PubMed  CAS  Google Scholar 

  12. S Portet O Arino J Vassy D Schoevaert (2003) ArticleTitleOrganization of the cytokeratin network in an epithelial cell J Theor Biol 7 IssueID223(3 313–333

    Google Scholar 

  13. True L.D. Principles of immunohistochemistry. In: Atlas of diagnostic immunohistopathology. Gawer Medical Publishing. New York. Tumors of the kidney, renal pelvis, and ureter. Atlas of tumor pathology. Armed forces institute of pathology, Bathesda, series 2, fasicle 12.

  14. V D’Agati M Trudel (1992) ArticleTitleLectin characterization of cystogenesis in the SBM transgenic model of polycystic kidney disease J Am Soc Nephrol 3 IssueID4 975–983 Occurrence Handle1280472 Occurrence Handle1:STN:280:ByyD1c7ntFc%3D

    PubMed  CAS  Google Scholar 

  15. B Angus S Kiberu J Purvis (1988) ArticleTitleCytokeratins in cervical dysplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PLL1 J. Pathology 155 71–75 Occurrence Handle1:STN:280:DyaL1c3ksV2huw%3D%3D

    CAS  Google Scholar 

  16. N Azumi H Batiffora (1987) ArticleTitleThe distribution of vimentin and keratin in epithelial and non-epithelial neoplasms. A comprehensive immunohistochemical study on formalin and alcohol fixed tumors Am J Clin Pathol 88 286–296 Occurrence Handle2443000 Occurrence Handle1:CAS:528:DyaL2sXlslWrtbY%3D

    PubMed  CAS  Google Scholar 

  17. GW Mierau JB Beckwith DA Weeks (1987) ArticleTitleUltrastructure and histogenesis of the renal tumors of childhood: an overview Ultrastruct Pathol 11 IssueID2–3 313–333 Occurrence Handle3035769 Occurrence Handle1:STN:280:DyaL2s3jsFOrsg%3D%3D

    PubMed  CAS  Google Scholar 

  18. MA Chidgey (1997) ArticleTitleDesmosomes and disease Histol Histopathol 12 IssueID4 1159–1168 Occurrence Handle9302575 Occurrence Handle1:STN:280:DyaK2svkvVWltw%3D%3D

    PubMed  CAS  Google Scholar 

  19. JR McMillan H Shimizu (2001) ArticleTitleDesmosomes: structure and function in normal and diseased epidermis J Dermatol 28 IssueID6 291–298 Occurrence Handle11476106 Occurrence Handle1:CAS:528:DC%2BD3MXmvValsLc%3D

    PubMed  CAS  Google Scholar 

  20. AP Kowalczyk EA Bornslaeger SM Norvell HL Palka KJ Green (1999) ArticleTitleDesmosomes: intercellular adhesive junctions specialized for attachment of intermediate filaments Int Rev Cytol 185 237–302 Occurrence Handle9750269 Occurrence Handle1:CAS:528:DyaK1MXlsVaj

    PubMed  CAS  Google Scholar 

  21. SB Jackson PH Strausbauch JL Finley D Laich KO Hewan-Lowe (2003) ArticleTitleDesmosomes and microvilli mean a lot: diagnosis of neoplasms of unknown origin using electron microscopy Ultrastruct Pathol 27 IssueID3 155–161 Occurrence Handle12775506

    PubMed  Google Scholar 

  22. MA Hayat (1989) Principles and techniques of Electron microscopy: Biological Application EditionNumber3 CRC press Boca Raton, USA 313–315

    Google Scholar 

  23. G Gonlusen M Ergin S Paydas N Tunali (2001) ArticleTitleThe expression of cytoskeletal proteins (alpha-SMA, vimentin, desmin) in kidney tissue: a comparison of fetal, normal kidneys, and glomerulonephritis Int Urol Nephrol 33 IssueID2 299–305 Occurrence Handle10.1023/A:1015226426000 Occurrence Handle12092643 Occurrence Handle1:STN:280:DC%2BD38zkvFynug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  24. Mittinen M. Keratin immunohistochemical: Update of applications and pitfalls. Pathol Annual; 28: part 1. Appleton and Lang, Norwak and Connecticut.

  25. MP Rastaldi F Ferrario L Giardino G Dell’Antonio C Grillo P Grillo F Strutz GA Muller G Colasanti G D’Amico (2002) ArticleTitletransition of tubular epithelial cells in human renal biopsies Kidney Int 62 IssueID1 137–146 Occurrence Handle10.1046/j.1523-1755.2002.00430.x Occurrence Handle12081572

    Article  PubMed  Google Scholar 

  26. B Eckes E Colucci-Guyon H Smola S Nodder C Babinet T Krieg P Martin (2000) ArticleTitleImpaired wound healing in embryonic and adult mice lacking vimentin J Cell Sci 113 IssueIDPt 13 2455–2462 Occurrence Handle10852824 Occurrence Handle1:CAS:528:DC%2BD3cXlt1GntL0%3D

    PubMed  CAS  Google Scholar 

  27. F Langa C Kress E Colucci-Guyon H Khun S Vandormael-Pournin M Huerre C Babinet (2002) ArticleTitleTeratocarcinomas induced by embryonic stem (ES) cells lacking vimentin: an approach to study the role of vimentin in tumorigenesis J Cell Sci 113 IssueIDPt 19 3463–3472

    Google Scholar 

  28. C Noack F Prols AJ Gamel W Rist MP Mayer B Brand-Saberi (2003) ArticleTitleRevisiting vimentin expression in early chick development Anat Embryol 206 IssueID5 391–397 Occurrence Handle12698363 Occurrence Handle1:CAS:528:DC%2BD3sXltFGnu7k%3D

    PubMed  CAS  Google Scholar 

  29. R Waldherr K Schwechheimer (1985) ArticleTitleCo-expression of cytokeratin and vimentin intermediate-sized filaments in renal cell carcinomas. Comparative study of the intermediate-sized filament distribution in renal cell carcinomas and normal human kidney Virchows Arch A Pathol Anat Histopathol 408 IssueID1 15–27 Occurrence Handle10.1007/BF00739959 Occurrence Handle2414900 Occurrence Handle1:STN:280:DyaL28%2FksFyitw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  30. HJ Grone K Weber E Grone U Helmchen M Osborn (1987) ArticleTitleCoexpression of keratin and vimentin in damaged and regenerating tubular epithelia of the kidney Am J Pathol 129 IssueID1 1–8 Occurrence Handle2444108 Occurrence Handle1:STN:280:DyaL1c%2FitFaiuw%3D%3D

    PubMed  CAS  Google Scholar 

  31. MQ Zhu ME De Broe EJ Nouwen (1996) ArticleTitleVimentin expression and distal tubular damage in the rat kidney Exp Nephrol 4 IssueID3 172–183 Occurrence Handle8773480 Occurrence Handle1:CAS:528:DyaK28Xkt1ehsLs%3D

    PubMed  CAS  Google Scholar 

  32. F Terzi R Maunoury E Colucci-Guyon C Babinet P Federici P Briand G Friedlander (1997) ArticleTitleNormal tubular regeneration and differentiation of the post-ischemic kidney in mice lacking vimentin Am J Pathol 150 IssueID4 1361–1371 Occurrence Handle9094992 Occurrence Handle1:CAS:528:DyaK2sXis12hsrY%3D

    PubMed  CAS  Google Scholar 

  33. H Okada S Ban S Nagao H Takahashi H Suzuki EG Neilson (2000) ArticleTitleProgressive renal fibrosis in murine polycystic kidney disease: an immunohistochemical observation Kidney Int 58 IssueID2 587–597 Occurrence Handle10.1046/j.1523-1755.2000.00205.x Occurrence Handle10916082 Occurrence Handle1:STN:280:DC%2BD3cvitFKntw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  34. C Arcangeletti M Olink-Coux R Minisini M Huesca C Chezzi K Scherrer (1992) ArticleTitlePatterns of cytodistribution of prosomal antigens on the vimentin and cytokeratin networks of monkey kidney cells Eur J Cell Biol 59 IssueID2 464–476 Occurrence Handle1283593 Occurrence Handle1:CAS:528:DyaK3sXhvVyhu7s%3D

    PubMed  CAS  Google Scholar 

  35. Bravo J, Quiroz Y, Pons H, Parra G, Herrera-Acosta J, Johnson RJ, Rodriguez-Iturbe B. Vimentin and heat shock protein expression are induced in the kidney by angiotensin and by nitric oxide inhibition. Kidney Int Suppl. 2003; (86): S46–51

  36. J Koeser SM Troyanovsky C Grund WW Franke (2003) ArticleTitleDe novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components Exp Cell Res 285 IssueID1 114–130 Occurrence Handle10.1016/S0014-4827(03)00016-8 Occurrence Handle12681292 Occurrence Handle1:CAS:528:DC%2BD3sXislCjtb8%3D

    Article  PubMed  CAS  Google Scholar 

  37. KJ Green CA Gaudry (2000) ArticleTitleAre desmosomes more than tethers for intermediate filaments? Nat Rev Mol Cell Biol 1 IssueID3 208–216 Occurrence Handle10.1038/35043032 Occurrence Handle11252896 Occurrence Handle1:CAS:528:DC%2BD3MXivVGjsL0%3D

    Article  PubMed  CAS  Google Scholar 

  38. DR Garrod (1995) ArticleTitleDesmosomes and cancer Cancer Surv 24 97–111 Occurrence Handle7553665 Occurrence Handle1:STN:280:DyaK28%2FisVSluw%3D%3D

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Urology and Nephrology Center, Mansoura University, Mansoura, Egypt

    Attalla F. El-Kott & Mahmoud A. El-Baz

  2. Department of zoology, Faculty of Science, Mansoura University, Egypt

    Mohamed F. Ismaeil

  3. Department of anatomy, Faculty of Medicine, Mansoura University, Egypt

    Moustafa M. Abd El-Moneim

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  1. Attalla F. El-Kott
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  2. Mohamed F. Ismaeil
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  3. Moustafa M. Abd El-Moneim
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  4. Mahmoud A. El-Baz
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Correspondence to Attalla F. El-Kott.

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El-Kott, A.F., Ismaeil, M.F., El-Moneim, M.M.A. et al. Histogenesis of Human Renal Cell Carcinoma by using Electron Microscopy and Immunohistochemical Techniques. Int Urol Nephrol 37, 439–445 (2005). https://doi.org/10.1007/s11255-004-6103-4

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  • DOI: https://doi.org/10.1007/s11255-004-6103-4

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