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Analysis of long non-coding RNAs in skeletal muscle of Bama Xiang pigs in response to heat stress

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Abstract

The aim of this study was to identify differentially expressed long non-coding RNAs (lncRNAs) molecules and predict their target genes related to muscle development and lipid metabolism in longissimus dorsi (LD) muscles of Bama Xiang pigs under constant heat stress. Ten male Bama Xiang pigs with an average initial body weight of 14 kg were randomly divided into control group (22°C) and heat stress (35 °C) group. The experiment lasted for 28 days. All the pigs were slaughtered at the end of the experiment, and LD muscles were collected for muscle quality analysis and transcriptome sequencing. Heat stress reduced meat quality of Bama Xiang pigs. lncRNAs in LD were identified systematically by deep RNA sequencing between the two groups. The results showed that 365 lncRNAs from the LD were identified, including 128 intergenic lncRNAs, 82 intronic lncRNAs, and 155 anti-sense lncRNAs. The differences lie in transcript of length, number of exons and wider size distribution, and expression level per KB fragment in three subtypes of lncRNAs. The three types of transposable elements coverage, including Line/L1, SINE/tRNA, and LTR/ERVL-MaLR, are the highest in mRNA and the three subtypes of lncRNAs in pigs. lncRNAs and mRNAs were different in comparison of features. The results predicted the target genes of the significant differentially expressed lncRNAs related to muscle development and lipid metabolism. This is the first study to expand the knowledge about muscle-related lncRNAs biology in Bama Xiang pigs under heat stress and will contribute to the development of alleviating the adverse effects of heat stress on pork quality targeting lncRNAs.

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Data will be made available on request.

Funding

This work was supported by the grants from the National Natural Science Foundation of China (31760672 and 31460606).

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Authors and Affiliations

  1. College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China

    Yanna Huang, Yujian Shen, Hui Zou & Qinyang Jiang

Authors
  1. Yanna Huang
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  2. Yujian Shen
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  3. Hui Zou
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  4. Qinyang Jiang
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Contributions

Yanna Huang: Conceptualization, methodology, investigation, data curation, formal analysis, funding acquisition, writing—original draft, and writing—review and editing. Yujian Shen: Investigation, data curation, and formal analysis. Hui Zou: Investigation, validation and data curation. Qinyang Jiang: Supervision, resources, software, visualization, and writing—review and editing

Corresponding author

Correspondence to Qinyang Jiang.

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Ethics approval

All experimental procedures used in this study were approved by the Institutional Animal Care and Welfare Committee of Guangxi University (No. Gxu-2019-184, Guangxi University, Nanning, China) and were carried in accordance with the UK Animals (Scientific Procedures), EU Directive 2010/63/EU for animal experiments.

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All the authors were consented for the “article entitled: Analysis of long non-coding RNAs in skeletal muscle of Bama Xiang pigs in response to heat stress” to be published in the journal Tropical animal health and production.

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Huang, Y., Shen, Y., Zou, H. et al. Analysis of long non-coding RNAs in skeletal muscle of Bama Xiang pigs in response to heat stress. Trop Anim Health Prod 53, 259 (2021). https://doi.org/10.1007/s11250-021-02701-7

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