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The first molecular detection of Clostridium perfringens from pneumonic cases associated with foot and mouth disease in cattle and buffalo in Egypt

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Abstract

Panting syndrome and respiratory infection have been recorded in complicated cases of foot and mouth disease (FMD) in cattle. However, investigations on the causative agents of respiratory disease in such cases are scarce. In this study, 30 animals (13 buffalo and 17 cattle) suffering from respiratory distress associated with signs of FMD were examined. Serum samples were collected and FMD infection was confirmed. Bacteriological examination of lungs from eight necropitized cases revealed the presence of C. perfringens. Multiplex polymerase chain reaction (mPCR) was performed on the positive samples followed by sequencing analysis. The alpha toxin gene (plc) of C. perfringens was identified in six cases. The present investigation highlights the role of clostridial infection as a complication of FMD in cattle and buffalo. This is the first report identifying the C. perfringens toxins from lung of animals with respiratory distress associated with FMD infection.

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References

  • Alexandersen, S., Zhang, Z., Donaldson, A., Garland, A., 2003. The pathogenesis and diagnosis of foot-and-mouth disease. Journal of Comparative Cathology, 129, 1–36.

    Article  CAS  Google Scholar 

  • Beveridge, T.J., 2001. Use of the Gram stain in microbiology. Biotechnic and Histochemistry, 76, 111–118.

    Article  CAS  PubMed  Google Scholar 

  • Brett, M., 1994. Outbreaks of food-poisoning associated with lecithinase-negative Clostridium perfringens. Journal of Medical Microbiology, 41, 405–407.

    Article  CAS  PubMed  Google Scholar 

  • Chandranaik, B.M., Hegde, R., Shivashankar, B.P., Giridhar, P., Muniyellappa, H.K., Kalge, R., Sumathi, B.R., Nithinprabhu, K., Chandrashekara, N., Manjunatha, V., 2015. Serotyping of foot and mouth disease virus and Pasteurella multocida from Indian gaurs (Bos gaurus), concurrently infected with foot and mouth disease and haemorrhagic septicaemia. Tropical Animal Health and Production, 47, 933–937.

    Article  PubMed  Google Scholar 

  • Chen, C.-H., Ho, S.-Y., Lin, K.-H., 2011. Necrotizing Pneumonia Associated with Septicemia Caused by Clostridium perfringens: A Case Report. Internal Medicine, 22, 287–291.

    Google Scholar 

  • Chhabra, R., Sharma, R., Kakker, N.K., 2004. Comparative immunogenecity of foot and mouth disease virus antigens in FMD-haemorrhagic septicaemia combined vaccine and FMD vaccine alone in buffalo calves. Indian Journal of Experimental Biology, 42, 259–264.

    CAS  PubMed  Google Scholar 

  • Constable, P.D., Hinchcliff, K.W., Done, S.H., Grünberg, W., 2016. Veterinary medicine-e-book: a textbook of the diseases of cattle, horses, sheep, pigs and goats. Elsevier Health Sciences.

  • Craven, C., 1989. Fatal Clostridium perfringens septicemia associated with gastrointestinal arteriovenous malformations (vascular ectasias). Archives of pathology and laboratory Medicine, 113, 534–535.

    CAS  PubMed  Google Scholar 

  • De Diego, M., Brocchi, E., Mackay, D., De Simone, F., 1997. The non-structural polyprotein 3ABC of foot-and-mouth disease virus as a diagnostic antigen in ELISA to differentiate infected from vaccinated cattle. Archives of Virology, 142, 2021–2033.

    Article  PubMed  Google Scholar 

  • Díaz-San Segundo, F., Rodríguez-Calvo, T., de Avila, A., Sevilla, N., 2009. Immunosuppression during acute infection with foot-and-mouth disease virus in swine is mediated by IL-10. PLoS One, 4, e5659.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fernandez-Miyakawa, M.E., Sayeed, S., Fisher, D.J., Poon, R., Adams, V., Rood, J.I., McClane, B.A., Saputo, J., Uzal, F.A., 2007. Development and application of an oral challenge mouse model for studying Clostridium perfringens type D infection. Infection and Immunity, 75, 4282–4288.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Francoz, D., Buczinski, S., Bélanger, A., Forté, G., Labrecque, O., Tremblay, D., Wellemans, V., Dubuc, J., 2015. Respiratory pathogens in Quebec dairy calves and their relationship with clinical status, lung consolidation, and average daily gain. Journal of Veterinary Internal Medicine, 29, 381–387.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Freedman, J.C., Theoret, J.R., Wisniewski, J.A., Uzal, F.A., Rood, J.I., McClane, B.A., 2015. Clostridium perfringens type A-E toxin plasmids. Research in Microbiology, 166, 264–279.

    Article  CAS  PubMed  Google Scholar 

  • Grubman, M.J., Baxt, B., 2004. Foot-and-mouth disease. Clinical Microbiology Reviews 17, 465–493.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gurjar, A., Hegde, N., Love, B., Jayarao, B., 2008. Real-time multiplex PCR assay for rapid detection and toxintyping of Clostridium perfringens toxin producing strains in feces of dairy cattle. Molecular and Cellular Probes, 22, 90–95.

    Article  CAS  PubMed  Google Scholar 

  • Hatheway, C.L., 1990. Toxigenic clostridia. Clinical microbiology reviews 3, 66–98.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Howard, B.G., 2007. Alimentary system. In: Pathologic basis of veterinary medicine. McGavin MD, Zachary JF, (ed.). (4thedn), Mosby Elsevier, USA 30.

  • Karasawa, T., Wang, X., Maegawa, T., Michiwa, Y., Kita, H., Miwa, K., Nakamura, S., 2003. Clostridium sordellii phospholipase C: gene cloning and comparison of enzymatic and biological activities with those of Clostridium perfringens and Clostridium bifermentans phospholipase C. Infection and Immunity, 71, 641–646.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Katayama, S., Matsushita, O., Minami, J., Mizobuchi, S., Okabe, A., 1993. Comparison of the alpha-toxin genes of Clostridium perfringens type A and C strains: evidence for extragenic regulation of transcription. Infection and Immunity 61, 457–463.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Knowles, N.J., Wadsworth, J., Reid, S.M., Swabey, K.G., El-Kholy, A.A., El-Rahman, A.O.A., Soliman, H.M., Ebert, K., Ferris, N.P., Hutchings, G.H., 2007. Foot-and-mouth disease virus serotype A in Egypt. Emerging Infectious Diseases 13, 1593.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Koneman, E., Allen, S., Janda, W., Schreckenberger, P., Winn, W., 1992. Diagnostic Microbiology, 4. baski. J В Lippincott Co, Philadelphia, USA.

  • Lee, H.-S., Lee, N.-H., Seo, M.-G., Ko, Y.-J., Kim, B., Lee, J.-B., Kim, J.-S., Park, S., Shin, Y.-K., 2013. Serological responses after vaccination of growing pigs with foot-and-mouth disease trivalent (type O, A and Asia1) vaccine. Veterinary Microbiology, 164, 239–245.

    Article  CAS  PubMed  Google Scholar 

  • Lorenz, T.C., 2012. Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. Journal of Visualized Experiments, 22, e3998.

  • Lyons, N.A., Alexander, N., Strk, K.D., Dulu, T.D., Rushton, J., Fine, P.E., 2015. Impact of foot-and-mouth disease on mastitis and culling on a large-scale dairy farm in Kenya. Veterinary Research, 46, 015–0173.

    Article  Google Scholar 

  • Moustafa, A.M., Ali, S.N., Bennett, M.D., Hyndman, T.H., Robertson, I.D., Edwards, J., 2017. A Case-control Study of Haemorrhagic Septicaemia in Buffaloes and Cattle in Karachi, Pakistan, in 2012. Transboundary and Emerging Diseases, 64, 520–527.

    Article  CAS  PubMed  Google Scholar 

  • Nakamura, s., Kimura, i., Kamakawa, k., Nishida, S., 1983. Taxonomic relationships among Clostridium novyi types A and B, Clostridium haemolyticum and Clostridium botulinum type C. Microbiology, 129, 1473–1479.

    Article  CAS  Google Scholar 

  • Palmacci, C., Antocicco, M., Bonomo, L., Maggi, F., Cocchi, A., Onder, G., 2009. Necrotizing pneumonia and sepsis due to Clostridium perfringens: a case report. Cases Journal, 2, 50.

    Article  PubMed  PubMed Central  Google Scholar 

  • Rweyemamu, M., Roeder, P., Mackay, D., Sumption, K., Brownlie, J., Leforban, Y., Valarcher, J.F., Knowles, N., Saraiva, V., 2008. Epidemiological patterns of foot-and-mouth disease worldwide. Transboundary and Emerging Diseases, 55, 57–72.

    Article  CAS  PubMed  Google Scholar 

  • Sasani, F., Marzban, H., Javanbakht, J., Moosakhani, F., Imanparast, M., 2013. The Relationship between Microscopic Lesions and Different Types of Clostridium perfringens and their Related Toxins by Sandwich ELISA in Cattle. Journal of Microbial and Biochemical Technology, 5, 034–038.

    Article  CAS  Google Scholar 

  • Sievers, F., Higgins, D.G. 2014. Clustal Omega, accurate alignment of very large numbers of sequences, In: Multiple Sequence Alignment Methods,. Springer, 105–116.

  • Siqueira, F.F., Almeida, M.O., Barroca, T.M., Horta, C.C., Carmo, A.O., Silva, R.O., Pires, P.S., Lobato, F.C., Kalapothakis, E., 2012. Characterization of polymorphisms and isoforms of the Clostridium perfringens phospholipase C gene (plc) reveals high genetic diversity. Veterinary Microbiology, 159, 397–405.

    Article  CAS  PubMed  Google Scholar 

  • Sutmoller, P., Barteling, S.S., Olascoaga, R.C., Sumption, K.J., 2003. Control and eradication of foot-and-mouth disease. VirusResearch, 91, 101–144.

    CAS  Google Scholar 

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S., 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tanabe, K., Jones, W., Barie, P., 1989. Clostridial sepsis and malignant disease. Surgery, Gynecology and Obstetrics, 169, 423–428.

    CAS  PubMed  Google Scholar 

  • Tsutsui, K., Minami, J., Matsushita, O., Katayama, S., Taniguchi, Y., Nakamura, S., Nishioka, M., Okabe, A., 1995. Phylogenetic analysis of phospholipase C genes from Clostridium perfringens types A to E and Clostridium novyi. Journal of Bacteriology, 177, 7164–7170.

  • Uzal, F., Kelly, W., Morris, W., Assis, R., 2002. Effects of intravenous injection of Clostridium perfringens type D epsilon toxin in calves. Journal of Comparative pathology, 126, 71–75.

    Article  CAS  PubMed  Google Scholar 

  • Venkataramanan, R., Bandyopadhyay, S., Oberoi, M., 2005. Present status and strategies for the control of transboundary and other economically important animal diseases in India: a review. The Indian Journal of Animal Sciences, 75.

  • Verma, S.C., Mahajan, N.K., Malik, G., Dahiya, J.P., 2004. An Epidemiological Study On Bovine Haemorrhagic Septicaemia In Haryana. Indian Journal of Animal Research, 38, 14–19.

    Google Scholar 

  • Yoo, H.S., Lee, S.U., Park, K.Y., Park, Y.H., 1997. Molecular typing and epidemiological survey of prevalence of Clostridium perfringens types by multiplex PCR. Journal of Clinical Microbiology, 35, 228–232.

    CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Medicine, Faculty of Veterinary Medicine, Aswan University, Aswan, 37916, Egypt

    Magdy Elgioushy

  2. Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt

    Mohamed Abdo Rizk & Sabry El-Khodery

  3. Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt

    Mohamed El-Adl

  4. Zewail City of Science and Technology, University of Science and Technology, Giza, Egypt

    Mohamed Elhadidy

  5. Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt

    Mohamed Elhadidy

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  1. Magdy Elgioushy
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  2. Mohamed Abdo Rizk
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  3. Mohamed El-Adl
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  4. Mohamed Elhadidy
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  5. Sabry El-Khodery
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Correspondence to Sabry El-Khodery.

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The authors declare that they have no competing interests.

Ethical disclosure

Farm owners approved and signed the informed consent forms for the study. All animal procedures implemented in this study complied with institutional guidlines obtained from ethics committee in Mansoura University, Egypt.

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Elgioushy, M., Rizk, M.A., El-Adl, M. et al. The first molecular detection of Clostridium perfringens from pneumonic cases associated with foot and mouth disease in cattle and buffalo in Egypt. Trop Anim Health Prod 51, 847–852 (2019). https://doi.org/10.1007/s11250-018-1763-8

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  • DOI: https://doi.org/10.1007/s11250-018-1763-8

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