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Detection and molecular characterization of porcine kobuvirus in piglets in 2009–2013 in northern Thailand

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Abstract

A total of 636 fecal samples collected from piglets with and without diarrhea during 2009 to 2013 were tested for porcine kobuvirus by RT-PCR. From a total of 528 fecal samples collected from piglets with diarrhea and 108 from healthy controls, 505 (95.6%) and 104 (96.3%) were positive for porcine kobuvirus, respectively. The detection rates of porcine kobuvirus were remarkable equally high in both diarrheic and healthy piglets. Phylogenetic analysis revealed that porcine kobuvirus strains detected in both symptomatic and asymptomatic piglets were genetically closely related to each other and also to other porcine kobuviruses reported worldwide. It was interesting to point out that one of the porcine kobuvirus strains isolated from piglet in our study was similar to a porcine-like bovine kobuvirus reference strain isolated previously in South Korea. This finding provided the evidence to support the interspecies transmission of kobuviruses between cattle and swine.

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Acknowledgements

This study was funded by the grants from the Faculty of Medicine, Chiang Mai University (grant number 83/2555), Center of Excellence (Emerging and Re-emerging Diarrheal Viruses Research Center), Chiang Mai University (grant number ST6392(11)/1089), Thailand.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Medicine, Chiang Mai University, 110 Intawarorose, Sriphoom, Muang, Chiang Mai, 50200, Thailand

    Watchaporn Chuchaona, Pattara Khamrin, Arpaporn Yodmeeklin, Kattareeya Kumthip & Niwat Maneekarn

  2. Animal House Unit, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

    Aphisek Kongkaew

  3. Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand

    Ratchaya Vachirachewin

  4. Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan

    Hiroshi Ushijima

Authors
  1. Watchaporn Chuchaona
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  2. Pattara Khamrin
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  3. Arpaporn Yodmeeklin
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  4. Aphisek Kongkaew
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  5. Ratchaya Vachirachewin
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  6. Kattareeya Kumthip
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  7. Hiroshi Ushijima
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  8. Niwat Maneekarn
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Corresponding author

Correspondence to Pattara Khamrin.

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Statement of animal rights

All applications during the study were performed according to animal rights, and the manuscript does not contain clinical studies or patient data.

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The authors declare that they have no conflict of interest.

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Chuchaona, W., Khamrin, P., Yodmeeklin, A. et al. Detection and molecular characterization of porcine kobuvirus in piglets in 2009–2013 in northern Thailand. Trop Anim Health Prod 49, 1077–1080 (2017). https://doi.org/10.1007/s11250-017-1298-4

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  • DOI: https://doi.org/10.1007/s11250-017-1298-4

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