Abstract
In search for low-cost, safe and environmentally benign plant-based alternatives to commercial pesticides, the efficacy of Lippia javanica aqueous leaf extracts in controlling ticks on cattle, acute oral toxicity in mice and phytochemistry were evaluated. L. javanica aqueous leaf extracts at 10% and 20% w/v were effective at controlling cattle ticks but not as good as an amitraz-based acaricide Tickbuster®. However, they can provide an effective tick control option where synthetic products are unavailable or unaffordable, particularly in remote parts of southern Africa. Peripheral blood samples collected showed no haemoparasites in treated cattle implying that animals did not suffer from clinical tick-borne diseases. The leaf aqueous extracts of L. javanica were tested for toxicity in BALB/c mice. While anecdotal evidence suggests L. javanica has low mammalian toxicity, within 48 h all mice fed with the L. javanica leaf aqueous extract at 12.5–37.5% v/v were lethargic, and overall mortality was 37.5% (n = 24). Thus, despite their apparent safety, water extracts of L. javanica leaves may have deleterious health implications on humans and animals if consumed at very high doses. Many compounds have been identified from L. javanica including an array of phenolic glycosides, flavonoids and essential oils but none of these are known to have acute toxic properties.
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Ameyaw, Y., 2009. A growth regulator for the propagation of Lippia multiflora Moldenke, a herbal for the management of mild hypertension in Ghana, Journal of Medicinal Plants Research, 3, 681-685
Anonymous, 1993. Animal Health Cattle-Cleansing Regulations, 1993. Supplement to the Zimbabwean Government Gazette, 20 August, 1993, (Government Printer, Harare)
Aslam, S.N., Stevenson, P.C., Kokubun, T. and Hall, D.R., 2009. Antibacterial and antifungal activity of cicerfuran and related 2-arylbenzofurans and stilbenes, Microbiological Research, 164, 191-195
Belmain, S.R. and Stevenson, P.C., 2001. Ethnobotanicals in Ghana: Reviving and modernising age-old farmer practice, Pesticide Outlook, 12 (6), 233-238
Belmain, S.R., Neal, G.E., Ray, D.E. and Golob, P., 2001. Insecticidal and vertebrate toxicity associated with ethnobotanicals used as post-harvest protectants in Ghana, Food Chemical Toxicology, 39, 287-291
Benavides, E., Hernández, G., Romero, N., Castro, A. and Rodrígues, B., 2001. Preliminary evaluation of Neem (Azadirachta indica) extracts as an alternative for cattle tick, Boophilus microplus control, Revista Colombiana de Entomologia, 27 (1-2), 1-8
Bizimenyera, E.S., Swan, G.E., Chikoto, H. and Eloff, J.N., 2005. Rationale for using Peltopharum africanum (Fabaceae) extracts in veterinary medicine, Journal of South African Veterinary Association, 76, 54-58
Chhabra, M.B. and Saxena, M.J., 1998. The use of phytotherapeutic agents for the control of acariasis in animals: a review, Journal of Veterinary Parasitology, 12 (1), 3-8
Chowdhury, J.U., Yusuf, M., Begum, J., Sultana, S.A. and Hussain, M.M., 2003. Composition and fungitoxic properties of the essential oil of Lippia javanica leaves, Indian Perfumer, 47 (4), 385-388
da Costa, Andrea S., Arrigoni-Blank, MD, Blank, A. F., de Mendonca, AB., Amancio, VF.1. and Ledo, A S., 2007. In vitro establishment of Lippia sidoides cham, Horticultura Brasileira, 25, 68-72
Eloff, J.N., 1998. Which extractant should be used for screening and isolation of antimicrobial components?, Journal of Ethnopharmacology, 60, 1-8
Futyoyma, D.J. and Agrawal, A.A., 2009. Macroevolution and the biological diversity of plants and herbivores, Proceedings of the National Academy of Sciences, 106, 18054-18061
Gunjal, K., Goodbody, S., Delbaere, J., Kenefick, E. and Rammala, V., 2009. FAO/WFP crop and food security assessment mission to Zimbabwe. Special Report, 22 June 2009, (Food and Agriculture Organisation of the United Nations, Rome; World Food Programme, Rome)
Isman, M.B., 2008. Perspective Botanical Insecticides: for richer for poorer, Pest Management Science, 64, 8–11
Jamzad, Z., Grayer, R.J., Kite, G.C., Simmonds. M.S.J., Ingrouille, M. and Jalili, A., 2003. Leaf surface flavonoids in Iranian species of Nepeta (Lamiaceae) and some related genera, Biochemical Systematics and Ecology, 31, 587-600
Kamanula, J., Sileshi, G.W., Belmain, S.R., Sola, P., Mvumi, B.M., Nyirenda, G.K.C., Nyirenda, S.P. and Stevenson, P.C. (2010) Farmers’ Insect pest management practices and pesticidal plant use for protection of stored maize and beans in Southern Africa. International Journal of Pest Management
Kelly, W.R., 1979. Veterinary clinical diagnosis. 2nd Ed., (Bailliere Tindall, London)
Kreier, J. P., 1994. Theileriasis. In: Parasitic Protozoa. 2nd edn, (Academic Press, Inc. Boston)
Levine, N.D., 1985. Veterinary Protozoalogy, (Iowa State University Press. Ames., Iowa)
Lewis, J.J., 1965. An introduction to pharmacology, (The Williams and Wilkins Company, Baltimore)
Lukwa, N., Molgaard, P., Furu, P. and Bogh, C., 2009. Lippia javanica (Burm F.) Spreng: its general constituents and bioactivity on mosquitoes, Tropical Biomedicine, 26 (1), 85-91
Mahmmod, Y.S., El-Balkemy, F.A., Yuan, Z.G., El-Mekkawy, M.F., Monazie, A.M. and Zhu, X.Q., 2010. Field evaluation of PCR assays for the diagnostic of tropical theileriosis in cattle and water buffaloes in Egypt, Journal of Animal and Veterinary Advances, 9 (4), 696-699
Manenzhe, N.J., Potgieter, N., van Ree, T., 2004. Composition and antimicrobial activities of volatile components of Lippia javanica, Phytochemistry, 65 (16), 2333-2336
Martin, M., McCorkle, M.C. and Mathias, E., 2001. Ethnoveterinary Medicine: An annotated bibliography of community animal healthcare, (ITDG Publishing, London)
Moreira, F.J.C., Santos, C.D.G. and Innecco, R., 2009. Hatching and mortality of second-stage juveniles of Meloidogyne incognita race 2 in essential plant oils, Revista Ciencia Agronomica, 40 (3), 441-448
Moyo, B. and Masika, P.J., 2009. Tick control methods used by resource-limited farmers and the effect of ticks on cattle in rural areas of the Eastern Cape Province, South Africa, Tropical Animal Health and Production, 41, 517-523
Mujovo, S.F., Hussein, A.A., Meyer, J.J.M., Fourie, B., Muthivhi, T. and Lall, N., 2008. Bioactive compounds from Lippia javanica and Hoslundia opposite, Natural Product Research, 22 (12), 1047-1054
Noamesi, B.K., Abebago, G.I. and Bamgbose, S.O., 1985a. Muscle relaxant properties of aqueous extract of Lippia multiflora, Planta Medica, 51, 253–255
Noamesi, B.K., Abebago, G.I. and Bamgbose, S.O., 1985b. The vascular actions of aqueous extract of Lippia multiflora, Planta Medica, 51, 256–258
Nyirenda, S.P., Sileshi, G.W., Belmain, S.R., Kamanula, J., Mvumi, B.M., Sola, P., Nyirenda, G.K.C., and Stevenson, P.C. (2010) Farmers’ ethno-ecological knowledge of vegetable pests and pesticidal plant use in northern Malawi and eastern Zambia, African Journal Agricultural Research
Nzira, L., Per, M., Peter, F. and Claus, B., 2009. Lippia javanica (Burm F) Spreng: its general constituents and bioactivity on mosquitoes, Tropical Biomedicine, 26 (1), 85-91
Olivier, D.K., Shikanga, E.A., Combrinck, S., Krause, R.W.M., Regnier, T. and Dlamini, T.P., 2010. Phenylethanoid glycosides from Lippia javanica, South African Journal of Botany, 76 (1), 58-63
Pascual, M.E., Slowing, E., Carretero, E., Sanchez, Mata, D. and Villar, A., 2001. Lippia: traditional uses, chemistry and pharmacology: A review, Ethnopharmacology, 76, 201-214
Pegram, R.G. Tatchell, R.J. de Castro, J.J., Chizyuka, H.G.B., Creek, M.J., McCosker, P.J., Moran, M.C. and Nigarura, G. (1993) Tick control: new concepts. World Animal Review, (FAO), No. 74-75, 2-11.
O’neill, R.T., 2006. On sample sizes to estimate the protective efficacy of a vaccine, Statistics in Medicine, 7 (12), 1279-1288.
Quirantes-Pine, R., Funes, L., Micol, V., Segura-Carretero, A. and Fernandez-Gutierrez, A., 2009. High-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight and ion-trap tandem mass spectrometry to identify phenolic compounds from a lemon verbena extract, Journal of Chromatography A, 1216, 5391-5397
Samie, A., Obi, C.L., Lall, N. and Meyer, J.J.M., 2009a. In-vitro cytotoxicity and antimicrobial activities, against clinical isolates of Campylobacter species and Entamoeba histolytica, of local medicinal plants from the Venda region, in South Africa, Annals of Tropical Medicine and Parasitology, 103 (2), 159-170
Samie, A., Housein, A., Lall, N. and Meyer, J.J.M., 2009b. Crude extracts of, and purified compounds from, Pterocarpus angolensis, and the essential oil of Lippia javanica: their in-vitro cytotoxicities and activities against selected bacteria and Entamoeba histolytica, Annals of Tropical Medicine and Parasitology, 103 (5), 427-439
Samie, A., Tambani, T., Harshfield, E., Green, E., Ramalivhana, J.N. and Bessong, P.O., 2010. Antifungal activities of selected Venda medicinal plants against Candida albicans, Candida krusei and Cryptococcus neoformans isolated from South African AIDS patients, African Journal of Biotechnology, 9 (20), 2965-2976
Sastry, G. A., 1983. Veterinary Clinical Pathology, 3 rd Ed., (CBS Publishers, New Delhi)
Shikanga, E., Regnier, T., Combrinck, S. and Botha, B., 2009. Polar Lippia extracts as alternatives for the postharvest control of Guazatine (R)-resistant strains of Penicillium digitatum in citrus, Fruits, 64, 75-82
Statistical Analysis System (SAS), 2006. Statistical Analysis System User’s Guide, (SAS Institute Inc, Raleigh, North Carolina)
Stevenson, P. C., Dyarathna, T. K., Belmain, S. R. and Veitch, N. C., 2009a. Bisdesmosidic saponins from Securidaca longepedunculata (Polygalaceae) with deterrent and toxic properties to Coleapteran storage pests, Journal of Agricultural and Food Chemistry, 57, 8860–8867
Stevenson, P.C. Muyinza, H., Hall, D.R., Porter, E.A. Farman, D.I., Talwana, H. Mwanga R.O.M., 2009b. Chemical basis for resistance in sweetpotato Ipomoea batatas to the sweetpotato weevil Cylas puncticollis, Pure and Applied Chemistry, 81, 141-151
Stevenson, P. C., Nyirenda, S., Sileshi, G., Kamanula, J., Mvumi, B., Sola, P., Simmonds, M., and Belmain, S. (2010). Southern African Pesticidal Plants (SAPP) Project. Caesalpinioid woodlands of Southern Africa: optimising the use of pesticidal plants. Final Technical Report, (Natural Resources Institute, University of Greenwich, Chatham)
Van Wyk, B.E., 2008. A broad review of commercially important southern African medicinal plants, Journal of Ethnopharmacology, 119 (3), 342-355
Viljoen, A.M., Subramoney, S., van Vuuren, S.F., Baser, K.H.C. and Demerci, B., 2005. The composition, geographical variation and antimicrobial activity of Lippia javanica (Verbenaeceae) leaf oils, Ethnopharmacology, 96 (1-2), 271-277
Acknowledgements
The authors acknowledge funding from the European Union, 9th European Development Fund through the Implementation and Coordination of Agricultural Research and Training (ICART) Programme administered by the Secretariat and African Caribbean and Pacific Science and Technology Programme (SADC). We also acknowledge the role of Southern Alliance for Indigenous Resources (SAFIRE); World Agro Forestry Centre (ICRAF); Department for Agricultural Research Services, Malawi; Mzuzu University, Malawi in shaping this study. The authors are grateful to Henderson Research Institute, Zimbabwe for supplying the cattle, facilities and technical support. Dr. M. Munyangani of Mazowe Veterinary College, Zimbabwe provided technical expertise in screening cattle blood samples for parasites. We are also grateful to Dr G.C. Kite at the Royal Botanic Gardens, Kew, UK for LC-MS analysis.
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Madzimure, J., Nyahangare, E.T., Hamudikuwanda, H. et al. Acaricidal efficacy against cattle ticks and acute oral toxicity of Lippia javanica (Burm F.) Spreng. Trop Anim Health Prod 43, 481–489 (2011). https://doi.org/10.1007/s11250-010-9720-1
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DOI: https://doi.org/10.1007/s11250-010-9720-1