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Comparative in vitro anti-tick efficacy of commercially available products and newly developed phyto-formulations against field collected and resistant tick lines of Rhipicephalus (Boophilus) microplus

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Abstract

Rhipicephalus (Boophilus) microplus is considered as one of the most widely distributed tick species ecto-parasitizing on livestock and causes fatal diseases with significant production loss. To address the problem of controlling acaricide resistant tick infestations on animals, attention has been paid to develop eco-friendly phyto-acaricides. The present study was undertaken to evaluate the comparative anti-tick activities of commercially available herbal and chemical products with chemically characterized phyto-formulations developed recently against field ticks and resistant tick lines of R. (B.) microplus. The chemical product Butox® Vet was found nearly passive against all the tested resistant tick lines. However, one of the commercial polyherbal product, Zerokeet® showed an efficacy (E%) of 41.8–75.4 % (\(\bar{X} = 63.5 \pm 7.4\,\%\)) using recommended dilution (1:2) against field ticks and resistant tick lines. However, the other commercial product, Erina® EP has very limited efficacy against all the tested tick. In comparison, the newly developed phyto-formulation, NBA/13/B/2 and NAC-01 conferred an E% of 82.4–91.3 % (\(\bar{X} = 85.9 \pm 2.1\,\%\)) and 62.3–94.6 % (\(\bar{X} = 80.0 \pm 8.1\,\%\)), respectively, against tested resistant ticks. Results indicated higher marketing potentiality of newly developed formulation in the existing tick problem scenario.

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Acknowledgments

The authors are grateful to the Indian Council of Agricultural Research, New Delhi for funding through the World Bank funded National Agricultural Innovation Project No. [NAIP/Comp-4/C2066/2008-09] and National Fund for Basic Strategic and Frontier Application Research in Agriculture Project No. [NFBSFARA/BSA-4004/2013-14].

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Authors and Affiliations

  1. Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, 243122, India

    K. G. Ajith Kumar, Anil Kumar Sharma, Sachin Kumar, D. D. Ray & Srikant Ghosh

  2. Pharmacognosy and Ethnopharmacology Division, CSIR- National Botanical Research Institute, Lucknow, UP, 226001, India

    A. K. S. Rawat & Sharad Srivastava

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  1. K. G. Ajith Kumar
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  2. Anil Kumar Sharma
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  3. Sachin Kumar
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  4. D. D. Ray
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Correspondence to Srikant Ghosh.

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Ajith Kumar, K.G., Sharma, A.K., Kumar, S. et al. Comparative in vitro anti-tick efficacy of commercially available products and newly developed phyto-formulations against field collected and resistant tick lines of Rhipicephalus (Boophilus) microplus . J Parasit Dis 40, 1590–1596 (2016). https://doi.org/10.1007/s12639-015-0736-3

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