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Distribution of drug resistance among enterococci and Salmonella from poultry and cattle in Ethiopia

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Abstract

Enterococci and Salmonella were isolated from feces of chicken in intensive poultry farms and cattle which are maintained following traditional practices. Their resistance to different antibiotics was also determined. A total of 298 enterococcal isolates consisting of Enterococcus faecium (49.6%), Enterococcus durans (26.9%), Enterococcus hirea (11.9%), and Enterococcus faecalis (11.5%) were obtained. Among the enterococci, resistance to erythromycin (Ery), clindamicin (Cli), amoxicillin (Amo), ampicillin (Amp), and cephalothin (Cep) was high. Resistance to vancomycin (Van) was detected in all enterococcal species. Over 80% of the isolates showed multiple drug resistance. The most dominant patterns in poultry were Amo/Amp/Cep/Pen and Amo/Amp/Cep/Cli/Pen/Van. Among isolates from cattle, Amo/Amp/Cep/Cli/Ery/Pen/Van and Amo/Amp/Cli/Ery/Pen/Van constituted the most dominant multiple resistance patterns. A total of 51 Salmonella isolates were obtained from poultry (43/280) and cattle (8/450). About 70% of the isolates had varying resistance to the tested antibiotics. Multiple drug resistance was observed in over 30% of the Salmonella isolates. The most frequent resistance pattern was Amo/Amp/Cip/Gen/Str in cattle and Amo/Amp/Cep/Cip/Gen/Kan/Str in poultry. Enteroccoccal and Salmonella isolates showed multiple resistance to those antibiotics used in human and veterinary medicine. The high frequency of isolation of resistant enterococci is indicative of the wide dissemination of antibiotic resistant bacteria in the farm environment.

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References

  • Aarestrup, F. M., Wegener C. H., Collignon P., 2008. Resistance in bacteria of the food chain: epidemiology and control strategies. Expert Rev Anti-infect Ther, 6: 733–750.

    Article  PubMed  Google Scholar 

  • Anderson, J. F., Parrish, T. D., Akhtar, M., Zurek, L., Hirt, H., 2008. Antibiotic resistance of Enterococci in American bison (Bison bison) from a nature preserve compared to that of Enterococci in pastured cattle, Appl Environ Microbiol, 74: 1726–1730.

    Article  CAS  PubMed  Google Scholar 

  • Akhi, M.T., Farzaneh, F., Oskouei M., 2009. Study of enterococcal susceptibility patterns isolated from clinical specimens in Tabriz, Iran, Pakistan J Med Sci, 25: 211–216.

    Google Scholar 

  • Barbour, E. K., Nabbut, N. H., 1982. Isolation of Salmonella and some other potential pathogens from two chicken breeding farms in Saudi Arabia, Avian Dis, 26, 234–244.

    Article  CAS  PubMed  Google Scholar 

  • Bonomo, R.A., Rossolini, G.M., 2008. Importance of antibiotic resistance and resistance mechanisms. Expert Rev Anti-infect Ther, 6: 549–550.

    Article  PubMed  Google Scholar 

  • Chingwaru, W., Mpuchane, SF., Gashe, BA., 2003. Enterococcus faecalis and Enterococcus faecium isolates from milk, beef, and chicken and their antibiotic resistance, Journal of Food Protection, 66, 931–936.

    CAS  PubMed  Google Scholar 

  • Consumers Union, 2003. Presence of antimicrobial-resistant pathogens in retail poultry products: a report on tests by members in Australia and the United States. An information paper presented by Consumers International to the Codex Committee on Residues of Veterinary Drugs in Foods 14th Session, Washington. http://www.consumersunion.org/about/

  • de Jong A, Bywater R, Butty P, Deroover E, Godinho K, Klein U, Marion H, Simjee S, Smets K, Thomas V, Vallé M, Wheadon A., 2009. A pan-European survey of antimicrobial susceptibility towards human-use antimicrobial drugs among zoonotic and commensal enteric bacteria isolated from healthy food-producing animals. J Antimicrob Chemother, 63:733–44

    Article  PubMed  Google Scholar 

  • Gaedirelwe OG, Sebunya TK., 2008. The Prevalence and antibiotic susceptibility of Salmonella sp. in poultry and ostrich samples from slaughter houses in Gaborone, Botswana. J Anim Vet Adv, 7: 1151–1154.

    Google Scholar 

  • Gregerson G., 1978. Rapid method for distinction of Gram negative from gram positive bacteria. European J Appl Microbiol, 5: 123–127.

    Article  Google Scholar 

  • Hao Van, T., Moutafis, G., Thuoc Tran, L., Coloe P. J., 2007. Antibiotic resistance in food-borne bacterial contaminants in Vietnam. Appl Environ Microbiol, 73: 7906–7911.

    Article  Google Scholar 

  • Hayes, J.R., English, L.L., Carr, L.E., Wagner, D.D., Joseph, S.W., 2004. Multiple antibiotic resistance of Enterococcus spp. isolated from commercial poultry production environments. Appl Environ Microbiol, 70: 6005–6011

    Article  CAS  PubMed  Google Scholar 

  • Jorgenson,J.H.; Turnidge, J.D.; Washington, J.A., 1999. Dilution and disc diffusion method. In: Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C., Yolken, R.H., (eds), Manual of Clinical Microbiology 7thed, American Society for Microbiology, Washington, DC pp. 1526–1543

    Google Scholar 

  • Katsunuma Y, Hanazumi M, Fujisaki H, Minato H, Kataoka Y, Sawada T, Hashimoto Y, Yonemochi C. 2008. Comparison of pulsed-field gel electrophoresis patterns of antimicrobial-resistant Escherichia coli and Enterococci isolates from the feces of livestock and livestock farmers in Japan. J Gen Appl Microbiol. 54:39–50

    Article  CAS  PubMed  Google Scholar 

  • Lukasova, J., Sustackova, A., 2003. Enterococci and antibiotic resistance. Acta Vet Brun, 72: 315–323

    Google Scholar 

  • Lundin, J. I., Dargatz, D. A., Wagner, B. A., Lombard, J. E., Hill, A. E., Ladely, S. R., Fedorka-Cray P. J., 2008. Antimicrobial Drug Resistance of Fecal Escherichia coli and Salmonella spp. Isolates from United States Dairy Cows, Foodborne Pathog Dis, 5: 7–19.

    Article  CAS  PubMed  Google Scholar 

  • Manson, JM., Amith, JM. Cook, GM., 2004. Persistence of vancomycin resistant in New Zealand broilers after discontinuation of avopracin use, Appl Environ Microbiol, 70: 5764–5768

    Article  CAS  PubMed  Google Scholar 

  • Mathew AG., Cissell R., Liamthong S., 2007. Antibiotic resistance in bacteria associated with food animals: a United States perspective of livestock production, Food borne pathog Dis, 4: 115–133.

    Article  CAS  Google Scholar 

  • Mengistu, A., 2005. FAO Country Pasture/Forage Resource Profiles. Ethipia.http://WWW.fao.org/WAICENT/FAOINFO/AGRICULT/AGP/doc/pasture/forage.htm

  • Molla, B., Mesfin, A., Alemayehu, D., 2003. Multiple antimicrobial resistant Salmonella serotypes isolated from chicken carcass and giblets in Debre Zeit and Addis Ababa, Ethiopia. Ethiop. J Health Dev, 17: 131–149

    Google Scholar 

  • Murugkar, H.V., Rahman, H., Ashok Kumar, Bhattacharyya, D., 2005. Isolation, phage typing and antibiogram of Salmonella from man and animals in Northeastern India. Indian J Med Res, 122: 237–242

    CAS  PubMed  Google Scholar 

  • Oloya, J., Theis, M., Doetkott, D., Dyer, N., Gibbs, P., Khaitsa, M.L., 2007. Evaluation of Salmonella occurrence in domestic animals and humans in North Dakota (2000–2005), Foodborne Pathog Dis, 4: 551–563.

    Article  CAS  PubMed  Google Scholar 

  • Pieskus, J., Pia Franciosini,M., Proietti, P.C., Reich, F., Kazeniauskas, E., Butrimaite-Ambrozeviciene, C., Mauricas, M., Bolder, N. 2008. Preliminary investigation on Salmonella spp. Incidence in meat chicken farms in Italy, Germany and the Netherlands. Int J Poul Sci, 7: 813–817.

    Article  Google Scholar 

  • Presi P, Stärk KD, Stephan R, Breidenbach E, Frey J, Regula G. 2009. Risk scoring for setting priorities in a monitoring of antimicrobial resistance in meat and meat products. Int J Food Microbiol, 130: 94–100

    Article  CAS  PubMed  Google Scholar 

  • Sahlström, L., Rehbinder’V., Albihn, A., Aspan, A., Bengtsson, B., 2009. Vancomycin-resistant Enterococci (VRE) in Swedish sewage sludge. Acta Vet Scand, 51:

  • Seong, C.N., Shim, E.S., Kim, S.M., Yoo, J.C., 2004. Prevalence and characterization of vancomycin-resistant enterococci in chicken intestines and humans of Korea, Arch Pharm Res, 27: 246–253

    Article  CAS  PubMed  Google Scholar 

  • Tejedor, M. T., Gonzalez, M. M., Toledo, L.P., Gomez, P. L., Martin Barrasa, J. L., 2001. Identification and antibiotic resistance of Faecal enterococci isolated from water samples, Int J Hyg Environ Health, 203: 363–368

    Article  Google Scholar 

  • Van Hoorebeke, S., Van Immerseel, F., De Vylder J., Ducatelle, R., Haesebrouck, F., Pasmans, F., de Kruif A., Dewulf J., 2008. Faecal sampling underestimates the actual prevalence of Salmonella in laying hen flocks, Zoon Pub Health, 56: 471–476.

    Article  Google Scholar 

  • Wallinga, D., Bermudez, N., Hopkins, E., 2002. Poultry on antibiotics: hazards to human health, 2nd edn. Sierra Club and IATP,pp27.

  • Wassenaar, T.M., Silley P., 2008. Antimicrobial resistance in zoonotic bacteria: lessons learned from host-specific pathogens. Anim Health Res Rev, 9: 177–186.

    Article  PubMed  Google Scholar 

  • Worku, A., 2005. Isolation of Multi-drug resistant enterococci in patients and health care workers in Tikur anbessa hospital. MSc thesis. Faculty of medicine, Addis Ababa University.

  • Yoshimura, H., Ishimaru, M., Endoh, Y.S., Kojima, A., 2004. Antimicrobial susceptibilities of Enterococci isolated from faeces of broiler and layer chickens. Lett Appl Microbiol 31: 427–432

    Article  Google Scholar 

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Acknowledgment

The first author thanks Sida/SAREC for the financial support.

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  1. Behailu Bekele

    Present address: Department of Food technology, Bahir Dar University, P.O. Box 26, Bahir Dar, Ethiopia

Authors and Affiliations

  1. Department of Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

    Behailu Bekele

  2. Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

    Mogessie Ashenafi

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Correspondence to Mogessie Ashenafi.

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Bekele, B., Ashenafi, M. Distribution of drug resistance among enterococci and Salmonella from poultry and cattle in Ethiopia. Trop Anim Health Prod 42, 857–864 (2010). https://doi.org/10.1007/s11250-009-9499-0

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