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Spread of the serotypes and antimicrobial resistance in strains of Salmonella spp. isolated from broiler

  • Food Microbiology - Research Paper
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Abstract

Serotypes and antimicrobial susceptibility of Salmonella’s isolates from broiler production chain were determined. A total of 239 isolated strains from chicken, carcasses, breeding environments, and slaughter was analyzed by disk diffusion test, in the period of 2009 to 2010. For antibiotics with a high number of resistant strains, the minimal inhibitory concentration (MIC) was performed. We identified 24 serotypes, being the most frequent, Minnesota (31.4%) and Infantis (22.6%). The highest percentages of resistance were obtained for sulfonamide (42.7%), followed by tetracycline with 37.6% and amoxicillin with 27.6%. From the total, 32 resistance profiles were identified, being 60.7% of the strains were resistant to at least one antibiotic. Of these, 31.7% of the isolates showed multidrug resistance profiles belonging to serovar Minnesota, Saintpaul, and S. enterica. The highest resistance was found in isolates from slaughterhouse (66.9%) and aviary (58.7%). A large number of strains showed MIC above the maximum tested concentration for the antibiotics amoxicillin and sulfamethoxazole. The high number of Salmonella’s resistant strains indicates the need for prudent use of these drugs in poultry production in order to reduce the occurrence and spread of antibiotic resistance profiles, and the risk that multiresistant strains isolated from broilers may pose a risk to human health.

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Funding

This study was financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Ministério da Agricultura Pecuária e Abastecimento (MAPA), and the Secretaria de Defesa Agropecuária (SDA), approved with the grant number 578372/2008-8, according to the Notice CNPq/MAPA/SDA no. 064/2008.

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Authors and Affiliations

  1. Laboratório de Biologia Molecular, Universidade de Uberaba, Av. Nenê Sabino 1801, Bairro Aeroporto, Uberaba, MG, 38055-500, Brazil

    Eliane Pereira Mendonça

  2. Laboratório de Biotecnologia Animal Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Rua Ceará, s/n, Bloco 2D, Sala 43, Campus Umuarama, Uberlândia, MG, Brazil

    Roberta Torres de Melo, Priscila Christen Nalevaiko, Guilherme Paz Monteiro, Belchiolina Beatriz Fonseca, Newton Nascentes Galvão & Daise Aparecida Rossi

  3. Faculdade de Ciências Farmacêuticas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Audecir Giombelli

Authors
  1. Eliane Pereira Mendonça
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  2. Roberta Torres de Melo
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  3. Priscila Christen Nalevaiko
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  4. Guilherme Paz Monteiro
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  8. Daise Aparecida Rossi
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Correspondence to Eliane Pereira Mendonça.

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Mendonça, E.P., de Melo, R.T., Nalevaiko, P.C. et al. Spread of the serotypes and antimicrobial resistance in strains of Salmonella spp. isolated from broiler. Braz J Microbiol 50, 515–522 (2019). https://doi.org/10.1007/s42770-019-00054-w

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  • DOI: https://doi.org/10.1007/s42770-019-00054-w

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