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The aflatoxin-detoxifizyme specific expression in the parotid gland of transgenic pigs

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Abstract

Producing aflatoxin-detoxifizyme (ADTZ) in pigs to control the AFT contamination of pig feed is a new research strategy by transgenic technology. In this study, transgenic pigs specifically expressing ADTZ gene in the parotid gland were successfully produced by somatic cell nuclear transfer technology. The ADTZ activity in saliva of 6 transgenic pigs was found to be 7.11 ± 2.63 U/mL. The feeding trial with aflatoxin (AFT) results showed that there were significant difference about the serum biochemical index such as total protein (TP), albumin (ALB), globulin (GLB) contents and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity and AFT residues in serum and liver between the pigs in the test treatment (transgenic pigs) producing ADTZ and those in the positive control (P < 0.05). In order to investigate the inheritance of the transgene, 11 G1 transgenic pigs were successfully obtained. The ADTZ activity in saliva of 11 G1 transgenic pigs was found to be 5.82 ± 1.53 U/mL. The feeding trial with AFT results showed that the serum biochemical index containing TP, ALB and GLB contents and ALT and AST activity and AFB1 residues in serum and liver of the pigs in the test treatment (transgenic pigs) producing ADTZ were significantly different than those in the positive control (P < 0.05). The above results demonstrated that ADTZ produced in transgenic pigs could improve the effect of the AFT contamination of feed on pigs.

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Acknowledgements

This work was supported by grants from Jinlin Provincial Nature Science Foundation (S2015042).

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  1. An-gang Lou and Jin-shun Cai have contributed equally to this work.

Authors and Affiliations

  1. College of Agriculture and Forestry Science, Linyi University, Shuangling Road, Linyi, 276005, China

    Li-zeng Guan

  2. Agriculture College, Yanbian University, Yanji, 133000, China

    An-gang Lou, Jin-shun Cai, Xue-mei Zhang, Cheng-dou Cui, Ying-shu Piao & Li-zeng Guan

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  1. An-gang Lou
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  2. Jin-shun Cai
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  3. Xue-mei Zhang
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  4. Cheng-dou Cui
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  6. Li-zeng Guan
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Correspondence to Li-zeng Guan.

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Lou, Ag., Cai, Js., Zhang, Xm. et al. The aflatoxin-detoxifizyme specific expression in the parotid gland of transgenic pigs. Transgenic Res 26, 677–687 (2017). https://doi.org/10.1007/s11248-017-0036-z

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  • DOI: https://doi.org/10.1007/s11248-017-0036-z

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