Abstract
The feasibility of using the pig parotid secretory protein promoter to drive the β-glucanase transgene expression in mouse parotid glands was examined in this study. The parotid gland-specific vector expressing β-glucanase gene (GLU, from Paenibacillus polymyxa CP7) was constructed. Transgenic mice were produced by the pronuclear microinjection. Both PCR and Southern blot analysis showed that the mice carried the β-glucanase gene and the β-glucanase gene could be stably inherited. Furthermore, RT-PCR and northern blot analysis indicated that it was specifically expressed in the parotid. The β-glucanase activity in the saliva was found to be 0.18 U/mL. After feeding a diet containing 2 % β-glucan, the average daily gain of transgenic was significantly higher than non-transgenic mice. The crude protein and crude fat concentration in faeces of transgenic mice were significantly reduced compared with that of the non-transgenic mice. These results suggest that the successful expression of foreign β-glucanase in the animal parotid would offer a promising biological approach to reduce the anti-nutritional effect of β-glucans in feed.
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Abbreviations
- PSP:
-
Parotid secretory protein promoter
- GLU:
-
Glucanase
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Acknowledgments
This work was supported by grants from the National Basic Research Program of China (973 Program, 2011CB944200), Chinese transgenic animal project (2011ZX08007-003 and 2011ZX08006-004). No additional external funding received for this study.
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Li-zheng Guan and Yu-ping Sun contributed equally to this work.
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Guan, Lz., Sun, Yp., Xi, Qy. et al. β-Glucanase specific expression in the parotid gland of transgenic mice. Transgenic Res 22, 805–812 (2013). https://doi.org/10.1007/s11248-012-9682-3
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DOI: https://doi.org/10.1007/s11248-012-9682-3