Abstract
n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P < 0.05) in both tissue and milk were observed.
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References
Arterburn LM, Hall EB, Oken H (2006) Distribution, interconversion, and dose response of n-3 fatty acids in humans. Am J Clin Nutr 83:1467S–1476S
Bongiovanni KD, Depeters EJ, Van Eenennaam AL (2007) Neonatal growth rate and development of mice raised on milk transgenically enriched with omega-3 fatty acids. Pediatr Res 62:412–416
Enser M, Hallett K, Fursey GAJ, Wood JD (1996) Fatty acid composition of English beef, lamb and pork at retail. Meat Sci 42:443–456
Foran JA, Good DH, Carpenter DO, Hamilton MC, Knuth BA, Schwager SJ (2005) Quantitative analysis of the benefits and risks of consuming farmed and wild salmon. J Nutr 135:2639–2643
Ge Y, Chen Z, Kang ZB, Cluette-Brown J, Laposata M, Kang JX (2002) Effects of adenoviral gene transfer of C. elegans n-3 fatty acid desaturase on the lipid profile and growth of human breast cancer cells. Anticancer Res 22:537–543
Goodnight SH, Harris WS Jr, Connor WE (1981) The effects of dietary omega 3 fatty acids on platelet composition and function in man: a prospective, controlled study. Blood 58:880–885
Horrocks LA, Yeo YK (1999) Health benefits of docosahexaenoic acid (DHA). Pharmacol Res 40:211–225
Indo Y, Tatemizo A, Abe Y, Suzuki I, Matsumoto K, Hosoi Y, Kinoshita M, Mikami K, Murata N, Iritani A, Saeki K (2009) Functional expression of a humanized gene for an ω-3 fatty acid desaturase from scarlet flax in transfected bovine adipocytes and bovine embryos cloned from the cells. Biochim Biophys Acta 1791:183–190
Kang ZB, Ge Y, Chen Z, Cluette-Brown J, Laposata M, Leaf A, Kang JX (2001) Adenoviral gene transfer of Caenorhabditis elegans n-3 fatty acid desaturase optimizes fatty acid composition in mammalian cells. Proc Natl Acad Sci USA 98:4050–4054
Kang JX, Wang J, Wu L, Kang ZB (2004) Transgenic mice: fat-1 mice convert n-6 to n-3 fatty acids. Nature 427:504
Kao BT, Lewis KA, DePeters EJ, Van Eenennaam AL (2006) Endogenous production and elevated levels of long-chain n-3 fatty acids in the milk of transgenic mice. J Dairy Sci 89:3195–3201
Knutzon DS, Thurmond JM, Huang YS, Chaudhary S, Bobik EG Jr, Chan GM, Kirchner SJ, Mukerji P (1998) Identification of delta 5-desaturase from Mortierella alpina by heterologous expression in Bakers’ yeast and canola. J Biol Chem 273:29360–29366
Lai L, Kang JX, Li R, Wang J, Witt WT, Yong HY, Hao Y, Wax DM, Murphy CN, Rieke A, Samuel M, Linville ML, Korte SW, Evans RW, Starzl TE, Prather RS, Dai Y (2006) Generation of cloned transgenic pigs rich in omega-3 fatty acids. Nat Biotechnol 24:435–436
Lu Y, Nie D, Witt WT, Chen Q, Shen M, Xie H, Lai L, Dai Y, Zhang J (2008) Expression of the fat-1 gene diminishes prostate cancer growth in vivo through enhancing apoptosis and inhibiting GSK-3 beta phosphorylation. Mol Cancer Ther 7:3203–3211
Marmer WN, Maxwell RJ, Williams JE (1984) Effects of dietary regimen and tissue site on bovine fatty acid profiles. J Anim Sci 59:109–121
Mitchell GE, Reed AW, Rogers SA (1991) Influence of feeding regimen on the sensory qualities and fatty acid contents of beef steaks. J Food Sci 56:1102–1103
Naylor RL, Goldburg RJ, Primavera JH, Kautsky N, Beveridge MC, Clay J, Folke C, Lubchenco J, Mooney H, Troell M (2000) Effect of aquaculture on world fish supplies. Nature 405:1017–1024
Park S, Johnson MA (2006) Awareness of fish advisories and mercury exposure in women of childbearing age. Nutr Rev 64:250–256
Pereira SL, Huang YS, Bobik EG, Kinney AJ, Stecca KL, Packer JC, Mukerji P (2004) A novel omega-3 fatty acid desaturase involved in the biosynthesis of eicosapentaenoic acid. Biochem J 378:665–671
Riediger ND, Othman RA, Suh M, Moghadasian MH (2009) A systemic review of the roles of n-3 fatty acids in health and disease. J Am Diet Assoc 109:668–679
Saeki K, Matsumoto K, Kinoshita M, Suzuki I, Tasaka Y, Kano K, Taguchi Y, Mikami K, Hirabayashi M, Kashiwazaki N, Hosoi Y, Murata N, Iritani A (2004) Functional expression of a Delta12 fatty acid desaturase gene from spinach in transgenic pigs. Proc Natl Acad Sci USA 101:6361–6366
Sakuradani E, Abe T, Iguchi K, Shimizu S (2005) A novel fungal omega 3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S–4. Appl Microbiol Biotechnol 66:648–654
Sidhu KS (2003) Health benefits and potential risks related to consumption of fish or fish oil. Regul Toxicol Pharmacol 38:336–344
Simopoulos AP (2003) Importance of the ratio of omega-6/omega-3 essential fatty acids: evolutionary aspects. World Rev Nutr Diet 92:1–22
Spychalla JP, Kinney AJ, Browse J (1997) Identification of an animal omega-3 fatty acid desaturase by heterologous expression in Arabidopsis. Proc Natl Acad Sci USA 94:1142–1147
Tao F, Zhu SW, Fan J, Cheng BJ (2006) Cloning and sequence analysis of maize FAD2 gene. Chin J Plant Physiol Mol Biol 32:649–656
Zhu G, Chen H, Wu X, Zhou Y, Lu J, Chen H, Deng J (2008) A modified n-3 fatty acid desaturase gene from Caenorhabditis briggsae produced high proportion of DHA and DPA in transgenic mice. Transgenic Res 17:717–725
Acknowledgments
This work was supported by Hi-Tech Research and Development Program of China (863 Programs, Nos. 2007AA100505 and 2008AA10Z159) and grant from Inner Mongolia University for Chang Jiang Scholars Program to GPL.
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Xia Wu, Hongsheng Ouyang and Biao Duan contributed equally to this work.
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Wu, X., Ouyang, H., Duan, B. et al. Production of cloned transgenic cow expressing omega-3 fatty acids. Transgenic Res 21, 537–543 (2012). https://doi.org/10.1007/s11248-011-9554-2
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DOI: https://doi.org/10.1007/s11248-011-9554-2