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Expression of Mycobacterium leprae HSP65 in tobacco and its effectiveness as an oral treatment in adjuvant-induced arthritis

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Abstract

Transgenic plants are able to express molecules with antigenic properties. In recent years, this has led the pharmaceutical industry to use plants as alternative systems for the production of recombinant proteins. Plant-produced recominant proteins can have important applications in therapeutics, such as in the treatment of rheumatoid arthritis (RA). In this study, the mycobacterial HSP65 protein expressed in tobacco plants was found to be effective as a treatment for adjuvant-induced arthritis (AIA). We cloned the hsp65 gene from Mycobacterium leprae into plasmid pCAMBIA 2301 under the control of the double 35S promoter from cauliflower mosaic virus. Agrobacterium tumefaciens bearing the pChsp65 plasmid was used to transform tobacco plants. Incorporation of the hsp65 gene was confirmed by PCR, reverse transcription-PCR, histochemistry, and western blot analyses in several transgenic lines of tobacco plants. Oral treatment of AIA rats with the HSP65 protein allowed them to recover body weight and joint inflammation was reduced. Our results suggest a synergistic effect between the HSP65 expressed protein and metabolites presents in tobacco plants.

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Abbreviations

AIA:

Adjuvant-induced arthritis

RA:

Rheumatoid arthritis

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Acknowledgments

We would like to thank Dr. T.P. Gillis from The National Hansen’s Disease Programs, Baton Rouge, Louisiana, USA for his kind donation of monoclonal antibody IIIE9 and Mr. Robert Butler from the National Institute for Medical Research, MRC, London, UK for his donation of recombinant Mycobacteriun leprae HSP65 protein.

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Authors and Affiliations

  1. Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B1, Francisco J Mújica S/N Col. Felicitas del Rio, CP 58060, Morelia, Michoacán, Mexico

    César Rodríguez-Narciso, Rafael Salgado-Garciglia & Rodolfo López-Gómez

  2. Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas IPN, Prol. Carpio y Plan de Ayala s/n, Col. Santo Tomas., CP 11340, México, D.F., Mexico

    Mayra Pérez-Tapia, Sergio Estrada-Parra & Iris Estrada-García

  3. Deparatamento de Ingeniería Genética, CINVESTAV Campus Guanajuato IPN, Km 9,5 Libramiento Norte Carr. Irapuato-León., Irapuato, Guanajuato, Mexico

    Rosa María Rangel-Cano

  4. Department of Biochemistry and Immunology, Medicine School of Ribeirão Preto, The Centre for Tuberculosis Research , University of São Paulo, São Paulo, SP, CEP 14049-900, Brazil

    Celio L. Silva

  5. Farmacología de Productos Naturales, Unidad Pediátrica, Centro Médico Nacional Siglo XXI, IMSS. Av. Cuauhtemoc 330, Col Doctores, CP 06725, México, D.F., Mexico

    Mariana Meckes-Fisher

Authors
  1. César Rodríguez-Narciso
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  2. Mayra Pérez-Tapia
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  3. Rosa María Rangel-Cano
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  4. Celio L. Silva
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  5. Mariana Meckes-Fisher
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  6. Rafael Salgado-Garciglia
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  7. Sergio Estrada-Parra
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  8. Rodolfo López-Gómez
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  9. Iris Estrada-García
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Corresponding author

Correspondence to Rodolfo López-Gómez.

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Rodríguez-Narciso, C., Pérez-Tapia, M., Rangel-Cano, R.M. et al. Expression of Mycobacterium leprae HSP65 in tobacco and its effectiveness as an oral treatment in adjuvant-induced arthritis. Transgenic Res 20, 221–229 (2011). https://doi.org/10.1007/s11248-010-9404-7

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  • DOI: https://doi.org/10.1007/s11248-010-9404-7

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