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Resistance against various fungal pathogens and reniform nematode in transgenic cotton plants expressing Arabidopsis NPR1

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Abstract

Cotton is an economically important crop worldwide that suffers severe losses due to a wide range of fungal/bacterial pathogens and nematodes. Given its susceptibility to various pathogens, it is important to obtain a broad-spectrum resistance in cotton. Resistance to several fungal and bacterial diseases has been obtained by overexpressing the Non-expressor of Pathogenesis-Related genes-1 (NPR1) in various plant species with apparently minimal or no pleiotropic effects. We examined the efficacy of this approach in cotton by constitutive expression of the Arabidopsis (Arabidopsis thaliana) NPR1 gene. The results show that NPR1-expressing lines exhibited significant resistance to Verticillium dahliae isolate TS2, Fusarium oxysporum f. sp. vasinfectum, Rhizoctonia solani, and Alternaria alternata. Interestingly, the transformants also showed significant resistance to reniform nematodes. Analysis of defense-related, biochemical and molecular responses suggest that when challenged with pathogens or certain systemic acquired resistance-inducing chemicals, the transgenic lines respond to a greater degree compared to the wild-type plants. Importantly, the basal activities of the defense-related genes and enzymes in uninduced transformants were no different than those in their non-transgenic counterparts. The results provide additional evidence supporting the role of NPR1 as an important part of the plant defense system and suggest a means to achieve broad-spectrum resistance to pathogens via genetic engineering.

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Acknowledgments

We thank Dr. Charles Kenerley for providing Rhizoctonia solani, and Alternaria alternata cultures that were used in this study. This research was supported by funds from Cotton Inc., Texas Higher Education Coordinating Board—Advanced Research Program (#000517-0005-2006), and Texas AgriLife Research.

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  1. Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX, 77843-2123, USA

    Vilas Parkhi, Vinod Kumar, LeAnne M. Campbell & Keerti S. Rathore

  2. Department of Soil & Crop Sciences, Texas A&M University, College Station, TX, 77843-2474, USA

    Keerti S. Rathore

  3. Southern Plains Agricultural Research Center, USDA-ARS, College Station, TX, 77845, USA

    Alois A. Bell

  4. Department of Biological Sciences, University of North Texas, Denton, TX, 76203, USA

    Jyoti Shah

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  1. Vilas Parkhi
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Parkhi, V., Kumar, V., Campbell, L.M. et al. Resistance against various fungal pathogens and reniform nematode in transgenic cotton plants expressing Arabidopsis NPR1 . Transgenic Res 19, 959–975 (2010). https://doi.org/10.1007/s11248-010-9374-9

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