Abstract
Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors eGFP expression was driven by the human EF1α promoter. The established transgenic animals were analyzed for eGFP expression by in vivo fluorescence imaging, PCR, histology and flow-cytometry. eGFP expression showed even distribution without mosaicism; however, tissue-dependent differences of eGFP expression were observed. Up to the sixth generation only one newborn showed eGFP inactivation. eGFP + transgenic bone marrow cells efficiently provided long-term haemopoietic repopulation in radiation chimeras, regenerating all bone marrow-derived lineages with eGFP + cells with distinct eGFP expression profiles. The established eGFP + BALB/c mouse strain is expected to be extremely useful in various immunological experiments.
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Acknowledgments
This work was supported by OTKA T 049034 and OMFB-00118/2008 to BZS, OTKA PD 78310 to KK. TC is a recipient of the Bolyai János Postdoctoral Fellowship of the Hungarian Academy of Sciences. The authors thank G. Takács for the artwork.
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Kvell, K., Czömpöly, T., Hiripi, L. et al. Characterisation of eGFP-transgenic BALB/c mouse strain established by lentiviral transgenesis. Transgenic Res 19, 105–112 (2010). https://doi.org/10.1007/s11248-009-9288-6
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DOI: https://doi.org/10.1007/s11248-009-9288-6