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Accumulation of vitamin E in potato (Solanum tuberosum) tubers

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Abstract

Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approaches were used to determine if increased levels of tocopherols can be accumulated in potato (Solanum tuberosum L.) tubers through metabolic engineering. Two transgenes were constitutively over-expressed in potato: Arabidopsis thaliana p-hydroxyphenylpyruvate dioxygenase (At-HPPD) and A. thaliana homogentisate phytyltransferase (At-HPT). α-Tocopherol levels in the transgenic plants were determined by high-performance liquid chromatography. In potato tubers, over-expression of At-HPPD resulted in a maximum 266% increase in α-tocopherol, and over-expression of At-HPT yielded a 106% increase. However, tubers from transgenic plants still accumulated approximately 10- and 100-fold less α-tocopherol than leaves or seeds, respectively. The results indicate that physiological and regulatory constraints may be the most limiting factors for tocopherol accumulation in potato tubers. Studying regulation and induction of tocopherol biosynthesis should reveal approaches to more effectively engineer crops with enhanced tocopherol content.

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References

  • Ahuja JKC, Goldman JD, Moshfegh AJ (2004) Current status of vitamin E nutriture. Ann NY Acad Sci 1031:387–390

    Article  PubMed  CAS  Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  • Brigelius-Flohé R, Traber MG (1999) Vitamin E: function and metabolism. FASEB J 13:1145–1155

    PubMed  Google Scholar 

  • Cahoon EB, Hall SE, Ripp KG, Ganzke TS, Hitz WD, Coughlan SJ (2003) Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content. Nature 21:1082–1087

    Article  CAS  Google Scholar 

  • Cheng Z, Sattler S, Maeda H, Sakuragi Y, Bryant DA, DellaPenna D (2003) Highly divergent methyltransferases catalyze a conserved reaction in tocopherol and plastoquinone synthesis in cyanobacteria and photosynthetic eukaryotes. Plant Cell 15:2343–2356

    Article  PubMed  CAS  Google Scholar 

  • Collakova E, DellaPenna D (2003) Homogentisate phytyltransferase activity is limiting for tocopherol biosynthesis in Arabidopsis. Plant Physiol 131:632–642

    Article  PubMed  CAS  Google Scholar 

  • Collakova E, DellaPenna D (2003a) The role of homogentisate phytyltransferase and other tocopherol pathway enzymes in the regulation of tocopherol synthesis during abiotic stress. Plant Physiol 133:930–940

    Article  CAS  Google Scholar 

  • DellaPenna D (2005) Progress in the dissection and manipulation of vitamin E synthesis. Trends Plant Sci 10:574–579

    Article  PubMed  CAS  Google Scholar 

  • Douches DS, Westedt AL, Zarka K, Grafius EJ (1998) Transformation of CryV-Bt transgene combined with natural resistance mechanisms for resistance to tuber moth in potato (Solanum tuberosum L.) HortScience 33:1053–1056

    CAS  Google Scholar 

  • Drewel BT, Giraud DW, Davy SR, Driskell JA (2006) Less than adequate vitamin E status observed in a group of preschool boys and girls living in the United States. J Nutr Biochem 17(2):132–138

    Article  PubMed  CAS  Google Scholar 

  • Ducreux LJM, Morris WL, Hedley PE, Shepherd T, Davies HV, Millam S, Taylor MA (2005) Metabolic engineering of high carotenoid potato tubers containing enhanced levels of beta-carotene and lutein. J Exp Bot 56:81–89

    PubMed  CAS  Google Scholar 

  • Fitter DW, Martin DJ, Copley MJ, Scotland RW, Langdale JA (2002) GLK gene pairs regulate chloroplast development in diverse plant species. Plant J 31:713–727

    Article  PubMed  CAS  Google Scholar 

  • Garcia I, Rodgers M, Pepin R, Hsieh T-F, Matringe M (1999) Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco. Plant Physiol 119:1507–1516

    Article  PubMed  CAS  Google Scholar 

  • Havaux M, Eymery F, Porfirova S, Rey P, Dörmann P (2005) Vitamin E protects against photoinhibition and photooxidative stress in Arabidopsis thaliana. Plant Cell 17:3451–3469

    Article  PubMed  CAS  Google Scholar 

  • Hofius D, Hajirezaei M-R, Geiger M, Tschiersch H, Melzer M, Sonnewald U (2004) RNAi-mediated tocopherol deficiency impairs photoassimilate export in transgenic potato plants. Plant Physiol 135:1256–1268

    Article  PubMed  CAS  Google Scholar 

  • Ischebeck T, Zbierzak AM, Kanwischer M, Dörmann P (2006) A salvage pathway for phytol metabolism in Arabidopsis. J Biol Chem 281:2470–2477

    Article  PubMed  CAS  Google Scholar 

  • Jiang Q, Wong J, Fyrst H, Saba JD, Ames BN (2004) gamma-Tocopherol or combinations of vitamin E forms induce cell death in human prostate cancer cells by interrupting sphingolipid synthesis. Proc Natl Acad Sci USA 101(51):17825–17830

    Article  PubMed  CAS  Google Scholar 

  • Kamal-Eldin A, Appelqvist LA (1996) The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipids 31:671–701

    Article  PubMed  CAS  Google Scholar 

  • Kanwischer M, Porfirova S, Bergmuller E, Dormann P (2005) Alterations in tocopherol cyclase activity in transgenic and mutant plants of Arabidopsis affect tocopherol content, tocopherol composition, and oxidative stress. Plant Physiol 137(2):713–723

    Article  PubMed  CAS  Google Scholar 

  • Karunanandaa B, Qi Q, Hao M, Baszis SR, Jensen PK, Wong Y-H H, Jiang J, Venkatramesh M, Gruys KJ, Moshiri F, Post-Beittenmiller D, Weiss JD, Valentin HE (2005) Metabolically engineered oilseed crops with enhanced seed tocopherol. Metab Eng 7:384–400

    Article  PubMed  CAS  Google Scholar 

  • Klaus D, Ohlrogge JB, Ekkehard Neuhaus H, Dormann P (2004) Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase. Planta 219:389–396

    Article  PubMed  CAS  Google Scholar 

  • Maeda H, Sakuragi Y, Bryant DA, DellaPenna D (2005) Tocopherols protect Synechocystis sp. strain PCC 6803 from lipid peroxidation. Plant Physiol 138:1422–1435

    Article  PubMed  CAS  Google Scholar 

  • McGrath JM, Jancso MM, Pichersky E (1993) Duplicate sequences with similarity to expressed genes in the genome of Arabidopsis thaliana. Theor Appl Genet 86:880–888

    Article  CAS  Google Scholar 

  • Monsanto Biotechnology Regulatory Services (2004) Monsanto Company, St. Louis, MI. Retrieved from http://gmo-crl.jrc.it/detectionmethods/MON-Art47dnaextraction.pdf May 2006

  • Morris WL, Ducreux LJM, Fraser PD, Millam S, Taylor MA (2006) Engineering ketocarotenoid biosynthesis in potato tubers. Metab Eng. Advance online publication.

  • Mueller LA, Zhang P, Rhee SY (2003) AraCyc: a biochemical pathway database for Arabidopsis. Plant Physiol 132:453–460

    Article  PubMed  CAS  Google Scholar 

  • National Potato Council (2006) Potato Statistics. Retrieved from http://www.nationalpotatocouncil.org/ March 8, 2006

  • Norris SR, Shen X, DellaPenna D (1998) Complementation of the Arabidopsis pds1 mutation with the gene encoding p-hydroxyphenylpyruvate dioxygenase. Plant Physiol 117:1317–1323

    Article  PubMed  CAS  Google Scholar 

  • Peñuelas J, Munné-Bosch S (2005) Isoprenoids: an evolutionary pool for photoprotection. Trends Plant Sci 10:166–169

    Article  PubMed  CAS  Google Scholar 

  • Provencher LM, Miao L, Sinha N, Lucas WJ (2001) Sucrose export defective1 encodes a novel protein implicated in chloroplast-to-nucleus signaling. Plant Cell 13:1127–1141

    Article  PubMed  CAS  Google Scholar 

  • Ricciarelli R, Tasinato A, Clement S, Ozer NK, Boscoboinik D, Azzi A (1998) alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state. Biochem J 15:243–249

    Google Scholar 

  • Rippert P, Scimemi C, Dubald M, Matringe M (2004) Engineering plant shikimate pathway for production of tocotrienol and improving herbicide resistance. Plant Physiol 134:92–100

    Article  PubMed  CAS  Google Scholar 

  • Römer S, Lubeck J, Kauder F, Steiger S, Adomat C, Sandmann G (2002) Genetic engineering of a zeaxanthin-rich potato by antisense inactivation and co-suppression of carotenoid epoxidation. Metab Eng 4:263–272

    Article  PubMed  CAS  Google Scholar 

  • SAS Institute (2005) The SAS system for Windows. Release 8.02. SAS Institute, Cary, NC

    Google Scholar 

  • Sattler SE, Cahoon EB, Coughlan SJ, DellaPenna D (2003) Characterization of tocopherol cyclases from higher plants and cyanobacteria. evolutionary implications for tocopherol synthesis and function. Plant Physiol 132:2184–2195

    Article  PubMed  CAS  Google Scholar 

  • Sattler SE, Gilliland LU, Magallanes-Lundback M, Pollard M, DellaPenna D (2004) Vitamin E is essential for seed longevity and for preventing lipid peroxidation during germination. Plant Cell 16:1419–1432

    Article  PubMed  CAS  Google Scholar 

  • Savidge B, Weiss JD, Wong Y-H H, Lassner MW, Mitsky TA, Shewmaker CK, Post-Beittenmiller D, Valentin HE (2002) Isolation and characterization of homogentisate phytyltransferase genes from Synechocystis sp. PCC 6803 and Arabidopsis. Plant Physiol 129:321–332

    Article  PubMed  CAS  Google Scholar 

  • Schneider C (2005) Chemistry and biology of vitamin E. Mol Nutr Food Res 49:7–30

    Article  PubMed  CAS  Google Scholar 

  • Shintani DK, DellaPenna D (1998) Elevating the vitamin E content of plants through metabolic engineering. Science 282:2098–2100

    Article  PubMed  CAS  Google Scholar 

  • Spychalla JP, Desborough SL (1990) Superoxide dismutase, catalase, and alpha-tocopherol content of stored potato tubers. Plant Physiol 94:1214–1218

    Article  PubMed  CAS  Google Scholar 

  • TIGR Gene Index Databases, The Institute for Genomic Research, Rockville, MD 20850 (URL: http://www.tigr.org/tdb/tgi) Accessed online October 2004

  • Tsegaye Y, Shintani DK, DellaPenna D (2002) Overexpression of the enzyme p-hydroxyphenolpyruvate dioxygenase in Arabidopsis and its relation to tocopherol biosynthesis. Plant Physiol Biochem 40:913–920

    Article  CAS  Google Scholar 

  • Valentin HE, Lincoln K, Moshiri F, Jensen PK, Qi Q, Venkatesh TV, Karunananadaa B, Baszis SR, Norris SR, Savidge B, Gruys KJ, Last RL (2006) The Arabidopsis vitamin E pathway gene5-1 mutant reveals a critical role for phytol kinase in seed tocopherol biosynthesis. Plant Cell 18:212–224

    Article  PubMed  CAS  Google Scholar 

  • Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, Thorne GM, Jiang J, Baszis SR, Levering CK, Aasen ED, Hao M, Stein JC, Norris SR, Last RL (2003) Engineering vitamin E content: from Arabidopsis mutant to soy oil. Plant Cell 15:3007–3019

    Article  PubMed  CAS  Google Scholar 

  • Yadav NR, Sticklen M (1995) Direct and efficient plant regeneration from leaf explants of Solanum tuberosum l. cv. Bintje. Plant Cell Rep 14:645–647

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The author gratefully acknowledges the generous contributions of Dr. Dean DellaPenna to this work, through the gift of the genes At-HPPD and At-HPT, by providing access to HPLC equipment, and for indispensable guidance.

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Author notes

  1. Elizabeth F. Crowell

    Present address: Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA, Route de Saint-Cyr, Versailles, 78026, Cedex, France

Authors and Affiliations

  1. Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI, 48824, USA

    Elizabeth F. Crowell & David S. Douches

  2. USDA-ARS, Sugarbeet and Bean Research Unit, East Lansing, MI, 48824, USA

    J. Mitchell McGrath

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  1. Elizabeth F. Crowell
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  2. J. Mitchell McGrath
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  3. David S. Douches
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Correspondence to Elizabeth F. Crowell.

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Crowell, E.F., McGrath, J.M. & Douches, D.S. Accumulation of vitamin E in potato (Solanum tuberosum) tubers. Transgenic Res 17, 205–217 (2008). https://doi.org/10.1007/s11248-007-9091-1

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  • DOI: https://doi.org/10.1007/s11248-007-9091-1

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