Summary
A two-step procedure was used for plant regeneration from in vitro grown leaf strips (2–3 mm wide) of cv. Bintje. Step I medium was designed with 2,4-dichlorophenoxycetic acid (2,4-D) at 0.0 or 9.0 μM, in combination with 2.28 μM kinetin (K), benzyl adenine (BA), zeatin (Z) or zeatin riboside (ZR). Step II media were 2,4-D-free media containing 5.78 μM gibberellic acid (GA3) and growth regulators similar to those of step I media. Leaf explants cultured in medium I containing zeatin riboside or zeatin for 6 days and then subcultured in medium II containing zeatin riboside produced numerous shoots without callus formation. Zeatin riboside containing step I and II media caused shoot regeneration in a high number (97.5±2.2) of explants. Approximately, 33.7±8.4 shoots were regenerated from each leaf explant.
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Abbreviations
- BA:
-
benzyladenine
- Z:
-
zeatin
- ZR:
-
zeatin riboside (trans isomer)
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
References
Cardi T, Carputo D, Frusciante L (1992) American Potato Journal 69:2–12
Cheng J, Bolyard MG, Saxena RC, Sticklen MB (1992) Plant Sci. 81:83–91
Edwards GA, Hepher A, Clerk SP, Boulter D (1991) Plant Mol. Biol. 17:89–100
Gill BS, Kam-Morgan LMW, Shepard JF (1987) J. Heredity 78:15–20
Hänisch ten Cate Ch H, SreeRamulu K (1987) Plant Sci. 49:209–216
Hänisch ten Cate Ch H, Ennik E, Roest S, SreeRamulu K, Dijkhuis P, De Groot B (1988) Thoer. Appl. Genet. 75:452–459
Hoekema A, Huisman MJ, Molendijk Lvan der Elzen PJM, Cornelissen BJC (1989) Bio/technology 7:273–278
Hulme JS, Higgins ES, Shields R (1992) Plant Cell Tissue and Organ Culture 31:161–167
Imai T, Aida R, Ishige T (1993) Plant Cell Rep. 12:299–302
Larkin PJ, Scowcroft WR (1981) Thoer. Appl. Genet. 60:197–214
MSTAT Computer Program (1991) Michigan State University, East Lansing, MI
Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497
Newell CA, Rozman R, Hinchee MA, Lawson EC, Haley L, Sanders P, Kaniewski NE, Tumer RB,
Horsch RB, Fraley RT (1991) Plant Cell Rep. 10:30–34
Sheerman S, Bevan MW (1988) Plant Cell Rep. 7:13–16
Synder GW, Belknap WR (1993) Plant Cell Rep. 12:324–327
Wenzler H, Mignery G, May G, Park W (1989) Plant Sci. 63:79–85
Wheeler VA, Evans NE, Foulger D, Webb KJ, Karp A, Franklin J, Bright SWJ (1985) Ann. Bot. 55:309–320
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Communicated by J. M. Widholm
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Yadav, N.R., Sticklen, M.B. Direct and efficient plant regeneration from leaf explants of Solanum tuberosum l. cv. Bintje. Plant Cell Reports 14, 645–647 (1995). https://doi.org/10.1007/BF00232730
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DOI: https://doi.org/10.1007/BF00232730