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Comparative spatial spread overtime of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) in fields of transgenic squash expressing the coat protein genes of ZYMV and WMV, and in fields of nontransgenic squash

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Abstract

The spatial and temporal patterns of aphid-vectored spread of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) were monitored over two consecutive years in plantings of nontransgenic and transgenic squash ZW-20H (commercial cv. Freedom II) and ZW-20B, both expressing the coat protein genes of ZYMV and WMV. All test plants were surrounded by nontransgenic plants that were mechanically inoculated with ZYMV or WMV, and served as primary virus source. Across all trials, none of the transgenic plants exhibited systemic symptoms upon infection by ZYMV and WMV but a few of them developed localized chlorotic dots and/or blotches, and had low mixed infection rates [4% (6 of 139) of ZW-20H and 9% (13 of 139) of ZW-20B], as shown by ELISA. Geostatistical analysis of ELISA positive transgenic plants indicated, (i) a lack of spatial relationship on spread of ZYMV and WMV for ZW-20H with flat omnidirectional experimental semivariograms that fitted poorly theoretical models, and (ii) some extent of spatial dependence on ZYMV spread for ZW-20B with a well structured experimental semivariogram that fitted poorly theoretical models during the first but not the second growing season. In contrast, a strong spatial dependence on spread of ZYMV and WMV was found for nontransgenic plants, which developed severe systemic symptoms, had prevalent mixed infection rates (62%, 86 of 139), and well-defined omnidirectional experimental semivariograms that fitted a spherical model. Geostatistical data were sustained by virus transmission experiments with Myzus persicae in screenhouses, showing that commercial transgenic squash ZW-20H alter the dynamics of ZYMV and WMV epidemics by preventing secondary plant-to-plant spread.

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References

  • Arce-Ochoa JP, Dainello F, Pike LM, Drews D (1995) Field performance comparison of two transgenic summer squash hybrids to their parental hybrid line. HortScience 30:492–493

    Google Scholar 

  • Blancard D, Lecoq H, Pitrat M (2004) A colour atlas of cucurbit diseases: observation, identification and control. Wiley and Sons, New York, NY, p 304

    Google Scholar 

  • Camann MA, Culbreath AK, Todd JW, Demski JW (1995) Spatial and temporal patterns of spotted wilt epidemics in peanut. Phytopathology 85:879–885

    Article  Google Scholar 

  • Castle SJ, Perring TM, Farrar CA, Kishaba AN (1992) Field and laboratory transmission of watermelon mosaic virus 2 and zucchini yellow mosaic virus by various aphid species. Phytopathology 82:235–240

    Article  Google Scholar 

  • Chellemi DO, Rohrbach KG, Yost RS, Sonoda RM (1988) Analysis of the spatial pattern of plant pathogens and diseased plants using geostatistics. Phytopathology 78:221–226

    Article  Google Scholar 

  • Clough GH, Hamm PB (1995) Coat protein transgenic resistance to watermelon mosaic virus and zucchini yellow mosaic virus in squash and cantaloupe. Plant Dis 79:1107–1109

    Article  CAS  Google Scholar 

  • David M (1977) Developments in geomathematics 2. Geostatistical ore reserve estimation. Elsevier, Amsterdam, p 364

    Google Scholar 

  • Davis RF, Mizuki MK (1987) Detection of cucurbit viruses in New Jersey. Plant Dis 71:40–44

    Article  Google Scholar 

  • Fuchs M, Gonsalves D (1995) Resistance of transgenic hybrid squash ZW-20 expressing the coat protein genes of zucchini yellow mosaic virus and watermelon mosaic virus 2 to mixed infections of both potyviruses. Bio/Technology 13:1466–1473

    Article  CAS  Google Scholar 

  • Fuchs M, Gonsalves D (1997) Genetic engineering. In: Rechcigl NA, Rechcigl JE (eds) Environmentally safe approaches to crop disease control. Lewis Publishers/CRC Press, Boca Raton, FL, pp 333–368

    Google Scholar 

  • Fuchs M, Klas FE, McFerson JR, Gonsalves D (1998a) Transgenic melon and squash expressing coat protein genes of aphid-borne viruses do not assist the spread of an aphid non-transmissible strain of cucumber mosaic virus. Trans Res 7:449–462

    Article  CAS  Google Scholar 

  • Fuchs M, Tricoli DM, Carney KJ, Schesser M, McFerson JR, Gonsalves D (1998b) Comparative virus resistance and fruit yield of transgenic squash with single and multiple coat protein genes. Plant Dis 82: 1350–1356

    Article  Google Scholar 

  • Fuchs M, Gal-On A, Raccah B, Gonsalves D (1999) Epidemiology of an aphid nontransmissible potyvirus in fields of nontransgenic and coat protein transgenic squash. Transg Res 99:429–439

    Article  Google Scholar 

  • Gonsalves D, Chee P, Provvidenti R, Seem R, Slightom JL (1992) Comparison of coat protein-mediated and genetically-derived resistance in cucumbers to infection by cucumber mosaic virus under field conditions with natural challenge inoculations by vectors. Bio/Technology 10:1562–1570

    Article  CAS  Google Scholar 

  • Gottwald TR (1992a). LCOR2-Spatial correlation analysis software for the personal computer. Plant Dis 76: 213–215

    Article  Google Scholar 

  • Gottwald TR (1992b) Spatial and spatio-temporal autocorrelation analysis of citrus canker epidemics in nurseries and groves in Argentina. Phytopathology 82:843–851

    Article  Google Scholar 

  • Gray SM, Moyer JW, Bloomfield P (1986) Two-dimensional distance class model for quantitative description of virus-infected plant distribution lattices. Phytopathology 76:243–247

    Google Scholar 

  • Habili N, Nutter FW (1997) Temporal and spatial analysis of grapevine leafroll-associated virus 3 in Pinot noir grapevines in Australia. Plant Dis 81:625–628

    Article  Google Scholar 

  • Journel AG (1987) Geostatistics for the Environmental Sciences. Project No CR 811893 Applied Earth Science Department, Stanford University, Stanford, CA, 94305

  • Kanieswki W, Lawson C, Sammons B, Haley L, Hart J, Delanay X, Tumer NE (1990) Field resistance of transgenic Russett Burbank potato to effects of infection by potato virus X and potato virus Y. Bio/Technology 8:750–754

    Article  Google Scholar 

  • Larkin RP, Gumpertz ML, Ristaino J (1995) Geostatistical analysis of Phytophthora. Epidemic development in commercial bell pepper fields. Phytopathology 85:191–203

    Article  Google Scholar 

  • Lecoustre R, Fargette D, Fauquet C, de Reffye P (1989) Analysis and mapping of the spatial spread of African cassava mosaic virus using geostatistics and kriging the technique. Phytopathology 79:913–920

    Article  Google Scholar 

  • Lecoq H, Pitrat M (1985) Specificity of the helper component-mediated aphid transmission of three potyviruses infecting muskmelon. Phytopathology 75:890–893

    Article  Google Scholar 

  • Madden LV, Pirone TP, Raccah B (1987) Analysis of spatial patterns of virus-diseased tobacco plants. Phytopathology 77:1409–1417

    Article  Google Scholar 

  • Malnoe P, Farinelli L, Collet GF, Reust W (1994) Small-scale field tests with transgenic potato cv. Bintje to test resistance to primary and secondary infections with potato virus Y. Plant Mol Biol 25:963–975

    Article  PubMed  CAS  Google Scholar 

  • Medley TL (1994) Availability of determination of nonregulated status for virus resistant squash. Fed Regist 59:64187–64189

    Google Scholar 

  • Reynolds KM, Madden LV, Ellis MA (1988) Spatio-temporal analysis of epidemic development of leather rot of strawberry. Phytopathology 78:246–252

    Article  Google Scholar 

  • Stein A, Kocks CG, Zadoks JC, Frinking HD, Ruissen MA, Meyers DE (1994) A geostatistical analysis of the spatio-temporal development of downy mildew epidemics in cabbage. Phytopathology 84:1227–1239

    Article  Google Scholar 

  • Steinlage TA, Hill JH, Nutter FW (2002) Temporal and spatial spread of Soybean mosaic virus (SMV) in soybeans transformed with the coat protein gene of SMV. Phytopathology 92:478–486

    Article  PubMed  CAS  Google Scholar 

  • Tanne E, Marcus R, Dubitzky E, Raccah B (1996) Analysis of progress and spatial pattern of corky bark in grapes. Plant Dis 84:1227–1239

    Google Scholar 

  • Tepfer M (2002) Risk assessment of virus-resistant transgenic plants. Annu Rev Phytopathol 40:467–491

    Article  PubMed  CAS  Google Scholar 

  • Tricoli D, Carney KJ, Russell PF, McMaster JR, Groff DW, Hadden KC, Himmel PT, Hubbard JP, Boeshore ML, Quemada HD (1995). Field evaluation of transgenic squash containing single or multiple virus coat protein gene constructs for resistance to cucumber mosaic virus, watermelon mosaic virus 2 and zucchini yellow mosaic virus. Bio/Technology 13:1458–1465

    Article  CAS  Google Scholar 

  • Ullman DE, Cho JJ, German TL (1991) Occurrence and distribution of cucurbit viruses in the Hawaiian Islands. Plant Dis 75:367–370

    Article  Google Scholar 

  • Zitter TA, Hopkins DL, Thomas CE (1996) Compendium of cucurbit diseases. APS Press, St Paul, MN

    Google Scholar 

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Acknowledgements

We are grateful to D. M. Tricoli from the Asgrow Seed Company for providing us with seeds of transgenic and nontransgenic squash. We thank Sheri Ecker-Day, Dave Hummer, and Vânia Souza for their excellent help, and Daniel Jarecke. This work was partially supported by a competitive grant from USDA’s Biotechnology Risk Assessment Grant Program (No 95-33120-1878). We also thank Dr. L.M. Yepes for critically reading the manuscript, and Drs. J.C. Zadoks and L.V. Madden for initial comments.

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  1. Dennis Gonsalves

    Present address: USDA-ARS, Pacific Basin Agricultural Research Center, 99 Aupuni ST, Suite 204, Hilo, HI, 96720, USA

Authors and Affiliations

  1. Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY, 14456, USA

    Ferdinand E. Klas, Marc Fuchs & Dennis Gonsalves

  2. Anton de Kom Universiteit van Suriname, Faculteit der Technologische Wetenschappen, Universiteitscomplex Leysweg, Building XVII, POB 9212, Paramaribo, Suriname

    Ferdinand E. Klas

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  1. Ferdinand E. Klas
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  2. Marc Fuchs
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Correspondence to Ferdinand E. Klas.

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Klas, F.E., Fuchs, M. & Gonsalves, D. Comparative spatial spread overtime of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) in fields of transgenic squash expressing the coat protein genes of ZYMV and WMV, and in fields of nontransgenic squash. Transgenic Res 15, 527–541 (2006). https://doi.org/10.1007/s11248-006-9001-y

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