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Enhanced Resistance to Botrytis cinerea Mediated by the Transgenic Expression of the Chitinase Gene ch5B in Strawberry

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Abstract

Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Colletotrichum acutatum, the etiological agent of the anthracnose disease, was not enhanced in the transgenic plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop.

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Abbreviations

KDa:

kilodalton

Kinetin:

6-furfurylaminopurine

nos :

nopaline synthase

npt II :

neomycin phosphotransferase II

PR:

pathogenesis related

35S:

35S Cauliflower Mosaic Virus promoter

TMV:

Tobacco Mosaic Virus

gusA :

β-glucuronidase gene

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Authors and Affiliations

  1. Departamento de Bioquímica de la Nutrición, Facultad de Bioquímica, Instituto de Química Biológica “Dr Bernabé Bloj”, Química y Farmacia; Instituto Superior de Investigaciones Biológicas (INSIBIO), Universidad Nacional de Tucumán, Chacabuco 461, CP, 4000, Tucumán, Argentina

    Gabriel R. Vellicce, Juan C. Díaz Ricci & Atilio P. Castagnaro

  2. Laboratory of Plant-Microorganism Interactions, Plant Division, Center for Genetic Engineering and Biotechnology (CIGB), Ave 31 e/158 & 190, PO Box 6162, 10600, Havana, Cuba

    Lázaro Hernández

  3. Sección Biotecnología, Estación Experimental Agroindustrial Obispo Colombres-Unidad Asociada al INSIBIO, CC No 9, 4101, Las Talitas, Tucumán, Argentina

    Gabriel R. Vellicce & Atilio P. Castagnaro

Authors
  1. Gabriel R. Vellicce
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  2. Juan C. Díaz Ricci
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  3. Lázaro Hernández
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  4. Atilio P. Castagnaro
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Correspondence to Atilio P. Castagnaro.

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Vellicce, G.R., Ricci, J.C.D., Hernández, L. et al. Enhanced Resistance to Botrytis cinerea Mediated by the Transgenic Expression of the Chitinase Gene ch5B in Strawberry. Transgenic Res 15, 57–68 (2006). https://doi.org/10.1007/s11248-005-2543-6

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  • DOI: https://doi.org/10.1007/s11248-005-2543-6

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