Abstract
We determined the primary structure of a tobacco β-1,3-glucanase gene. The β-1,3-glucanase gene has a single large intron, and the intron separates coding regions of the signal peptide and the mature enzyme. Analysis of the 5′-flanking region sequence revealed an 11 bp GC-rich element with perfect homology to the putative regulatory sequence of tobacco chitinase genes. RNA blot analysis showed that levels of mRNAs of β-1,3-glucanase and chitinase are coordinately increased in response to ethylene and salicylic acid. Accumulation of β-1,3-glucanase mRNA in suspension-cultured cells is rapidly induced at late logarithmic growth phase. Members of the tobacco β-1,3-glucanase gene families are classified into two subfamilies. One of the subfamilies appeared to be transcriptionally inactive.
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Ohme-Takagi, M., Shinshi, H. Structure and expression of a tobacco β-1,3-glucanase gene. Plant Mol Biol 15, 941–946 (1990). https://doi.org/10.1007/BF00039434
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DOI: https://doi.org/10.1007/BF00039434