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Culture condition optimization and FT-IR analysis of Polygonum multiflorum Thunb. adventitious root cultures grown in an air-lift bioreactor system

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Abstract

Bioreactor cultures have been used for biomass production and bioactive compounds accumulation in adventitious root cultures of medicinal plants. In this study, we evaluated the effects of different auxin types [indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)] and concentrations (0.5, 1.0, 2.0 and 4.0 mg·L−1), Murashige and Skoog (MS) medium salt strength (0.25, 0.5, 0.75, 1.0, 1.5 and 2X), and sucrose concentrations (0, 1.5, 3, 5, 7 and 10%) on Polygonum multiflorum Thunb. adventitious root cultures in a 3-L balloon-type bubble bioreactor (BTBB). IBA (1, 2, and 4 mg·L−1) was more effective than NAA in promoting root growth. Additionally, low MS salt strength (0.25 and 0.5X MS) increased the accumulation of total phenolics and flavonoids but reduced biomass accumulation. Four weeks of culture in full-strength MS medium supplemented with 2 mg·L−1 IBA and 5% sucrose resulted in the highest root biomass [98.46 g·L−1 fresh weight (FW); 13.46 g·L−1 dry weight (DW)] and bioactive compounds accumulation (total phenolics compounds, 53.08 mg·g−1 DW; total flavonoids, 25.10 mg·g−1 DW). To determine whether metabolic fingerprinting of whole-cell extracts could be used to compare metabolic equivalence of P. multiflorum root samples, we treated adventitious roots with different culture conditions, and analyzed the treated adventitious roots and natural roots by Fourier transform infrared (FT-IR) spectroscopy. The results showed that the metabolic pattern of adventitious root samples was similar under different culture conditions; however, these samples could be discriminated from each other in pilot-scale bioreactors. Overall, our study provides useful information for industrial-scale cultivation of P. multiflorum adventitious roots.

Key message

The optimum condition for bioreactor culture of Polygonum multiflorum is full-strength MS medium supplemented with 2 mg·L-1 IBA and 5% sucrose after 4 weeks of culture. FT-IR analysis can rapidly discriminate among root samples based on total metabolite equivalence.

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Acknowledgments

Authors were supported by Brain Korea (BK) 21 Plus Program through the National Research Foundation (NRF) of Korea.

Funding

This work was supported by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant Number 315013–4).

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Authors and Affiliations

  1. Institute for Global Health Innovations, Duy Tan University, Da Nang, 550000, Vietnam

    Thanh-Tam Ho

  2. Faculty of Pharmacy, Duy Tan University, Da Nang, 550000, Vietnam

    Thanh-Tam Ho

  3. Department of Horticulture, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Kim-Cuong Le & So-Young Park

  4. Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 901 83, Umeå, Sweden

    Kim-Cuong Le

  5. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 56212, Republic of Korea

    Suk-Weon Kim

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  1. Thanh-Tam Ho
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Contributions

Thanh-Tam Ho, Kim-Cuong Le contributed to data acquisition and manuscript writing. Suk-Weon Kim interpreted the data and revised the intellectual content. So-Young Park made substantial contributions to the conception and design of the study. All authors have read and agreed to the published version of the manuscript.

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Ho, TT., Le, KC., Kim, SW. et al. Culture condition optimization and FT-IR analysis of Polygonum multiflorum Thunb. adventitious root cultures grown in an air-lift bioreactor system. Plant Cell Tiss Organ Cult 144, 371–381 (2021). https://doi.org/10.1007/s11240-020-01961-9

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