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LlDREB1G, a novel DREB subfamily gene from Lilium longiflorum, can enhance transgenic Arabidopsis tolerance to multiple abiotic stresses

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Abstract

In plants, most DREB1s/CBFs proteins with a single AP2/ERF domain participate in responses to abiotic stresses. Here, we cloned a DREB subfamily gene from Lilium longiflorum (designated as LlDREB1G). Sequence analyses revealed that it is composed of 219 amino acids, and contains an AP2/ERF domain, a putative nuclear localization signal (NLS), a DSAW/R and LWSY motif. LlDREB1G, which was localized to the nucleus, showed strongly induced expression under low temperature, and was also stimulated by mechanical agitation, heat shock, ABA and H2O2 stresses. Moreover, the LlDREB1G gene had an obvious circadian rhythm and organ specificity in expression. The LlDREB1G transcription factor was functional in the heterologous system (Arabidopsis). Transgenic Arabidopsis plants with higher transcription of LlDREB1G gene exhibited increased acquired thermotolerance and resistance to freezing and dehydration stresses. Besides, the transgenic seeds were insensitive to exogenous ABA treatment than those of the wild type (WT) in germination experiments. Compared with the WT seedlings, the seedlings of transgenic Arabidopsis overexpressing LlDREB1G showed strongly upregulated expression of stress-related genes after cold, drought or acclimation to heat shock treatments. Furthermore, overexpression of LlDREB1G increased the proline levels and survival rates, but decreased the electrolyte leakage and H2O2 accumulation in the transgenic plants after freezing, dehydration and high temperature treatments. These results imply that LlDREB1G gene may be involved in the responses to multiple abiotic stresses in an ABA-dependent and -independent manner.

Key message

LlDREB1G gene, a DREB subfamily gene from Lilium longiflorum, may be involved in the responses to multiple abiotic stresses in an ABA-dependent and -independent manner.

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Abbreviations

ABA:

Abscisic acid

ABRE:

ABA responsive elements

ATSR:

Acquired thermotolerance after short recovery

BT:

Basal thermotolerance

CAT:

Catalase

DAB:

3, 3′-Diaminobenzidine

DAPI:

4′, 6-Diamidino-2-phenylindole

DREB:

Dehydration responsive element binding protein

GFP:

Green fluorescent protein

HS:

Heat shock

HSF:

Heat stress transcription factor

HSP:

Heat shock protein

LTRE:

Low temperature responsive elements

MV:

Methyl viologen

NAA:

α-naphthaleneacetic acid

NLS:

Nuclear localization signal

ORF:

Open reading frame

QPCR:

Quantitative real-time PCR

RACE:

Rapid amplification of cDNA ends

ROS:

Reactive oxygen species

TAIL-PCR:

Thermal asymmetric interlaced PCR

WT:

Wild type

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Acknowledgements

This work was supported by grants from the Special Fund for Agro-scientific Research in the Public Interest of China (Grant No. 201203071). We thank all colleagues in our laboratory for constructive discussion and technical support.

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China

    Baojun Liu, Ying Zhou, Wu Lan, Qin Zhou, Fei Li, Feng Chen & Manzhu Bao

  2. Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou, 510405, China

    Guofeng Liu

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  1. Baojun Liu
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  2. Ying Zhou
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  3. Wu Lan
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Contributions

GFL and MZB designed the study. BJL and WL wrote the paper. BJL, YZ, QZ, FL, FC performed the research and analyzed the data. All authors discussed the results and approved the final form of the manuscript.

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Correspondence to Guofeng Liu.

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Liu, B., Zhou, Y., Lan, W. et al. LlDREB1G, a novel DREB subfamily gene from Lilium longiflorum, can enhance transgenic Arabidopsis tolerance to multiple abiotic stresses. Plant Cell Tiss Organ Cult 138, 489–506 (2019). https://doi.org/10.1007/s11240-019-01644-0

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