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An aseptic rapid propagation system for obtaining plumbagin of Ceratostigma willmottianum Stapf

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Abstract

Ceratostigma willmottianum Stapf is an endemic species of China. Although it has high ornamental and medical value, its use is greatly limited by the scarcity of germplasm resources. To protect the wild resources and obtain more plumbagin, a protocol for aseptic rapid propagation of C. willmottianum was developed and the plumbagin content was quantified. The results showed that Murashige and Skoog (MS) medium with 6-benzylaminopurine (6-BA, 0.5 mg L− 1) and 1-naphthaleneacetic acid (NAA, 0.1 mg L− 1) was suitable for axillary bud induction with an induction rate of 82.74%, while MS medium with higher 6-BA (2.0 mg L− 1) and NAA (0.1 mg L− 1) was suitable for multiplication with a propagation coefficient of 2.91. Additionally, 1/2 MS medium with indole-3-butyric acid (IBA, 0.5 mg L− 1) was suitable for rooting, with rooting rate of 94.44 ± 1.93%. High-performance liquid chromatography (HPLC) analysis revealed the tendency of the plumbagin content to decrease in the following order: root had the highest content, followed by the whole-plant, stem and leaf. Notably, the plumbagin content in 1-month-old plantlets was higher than that in 1- and 2-year-old plants, especially in the roots (19.94 mg g− 1 DW). Overall, an aseptic rapid propagation system of C. willmottianum was successfully established for the first time and the roots of 1-month-old plantlets were used to obtain plumbagin rapidly.

Key message

An aseptic rapid propagation system of Ceratostigma willmottianum was established successfully and plumbagin content was quantified by HPLC in this study.

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Acknowledgements

This work was supported by the Breeding Project of Sichuan Province the 13th Five-Year Plan Tackle Key Problems, China (No. 2016NYZ0038). Special thanks to the American Journal Experts-AJE Company for editing the language of the original manuscript.

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Authors and Affiliations

  1. College of Landscape Architecture, Sichuan Agricultural University, Chengdu, 611130, China

    Ju Hu, Suping Gao, Meiting Hong, Yuan Zhu & Di Hu

  2. Institute of Landscape Architecture, Sichuan Agricultural University, Chengdu, 611130, China

    Suping Gao, Shiliang Liu & Ting Lei

  3. College of Science, Sichuan Agricultural University, Ya’an, 625014, China

    Yichao Wu & Li Zhang

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  1. Ju Hu
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Contributions

JH, SPG, and LZ designed the research. JH, MTH, YZ, YCW, and DH conducted the experiments. JH, YZ, and YCW analyzed the data. JH wrote the manuscript, SLL and TL proposed helpful suggestions and helped to revise the manuscript carefully in the course of major revision and minor repairs. All authors read and approved the manuscript.

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Correspondence to Suping Gao.

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The authors declare that they do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

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The experiments were performed according to the current laws of China.

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Communicated by Pamela J. Weathers.

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Hu, J., Gao, S., Liu, S. et al. An aseptic rapid propagation system for obtaining plumbagin of Ceratostigma willmottianum Stapf. Plant Cell Tiss Organ Cult 137, 369–377 (2019). https://doi.org/10.1007/s11240-019-01577-8

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