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Protocol for bonediol production in Bonellia macrocarpa hairy root culture

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Abstract

The aim of this protocol was for Bonellia macrocarpa hairy root establishment and bonediol production in transformed root cultures. Cotyledons, hypocotyls and roots were used as explants for hairy root induction. Fluorescence microscopy was used for detected tandem dimer of the tomato protein (TDT). A PCR analysis for rolC was used to confirm the presence of these genes. Identification and quantification of bonediol was made for HPLC. Hypocotyls showed greater transformation efficiency (25%). Fluorescence microscopy and PCR analysis confirm genetic transformation. Bonedio production was higher in hairy roots than in complete plants.

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Acknowledgements

Authors thanks Tecnológico Nacional de México and Dr. Sergio Rubén Peraza Sánchez for standard bonediol. LARR received a scholarship from CONACYT.

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Authors and Affiliations

  1. Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana Km. 1080, Tuxtla Gutiérrez, Chiapas, Mexico

    Luis Alberto Ruiz-Ramírez, Víctor Manuel Ruíz-Valdiviezo, María Celina Lujan-Hidalgo & Federico Antonio Gutiérrez-Miceli

  2. CONACYT-TecNM-Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana Km. 1080, Tuxtla Gutiérrez, Chiapas, Mexico

    Peggy Elizabeth Álvarez-Gutiérrez

  3. Unidad de Bioquímica y Biología Molecular, Centro de Investigación Científica de Yucatán A.C., Calle 43 No. 130 x 32 Y 34, Colonia Chuburná de Hidalgo, C.P. 97205, Mérida, Yucatán, Mexico

    Gregorio del Carmen Godoy-Hernández & Elidé Avilés-Berzunza

Authors
  1. Luis Alberto Ruiz-Ramírez
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  2. Gregorio del Carmen Godoy-Hernández
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  3. Peggy Elizabeth Álvarez-Gutiérrez
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  4. Víctor Manuel Ruíz-Valdiviezo
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  5. María Celina Lujan-Hidalgo
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  6. Elidé Avilés-Berzunza
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  7. Federico Antonio Gutiérrez-Miceli
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Corresponding authors

Correspondence to Gregorio del Carmen Godoy-Hernández or Federico Antonio Gutiérrez-Miceli.

Additional information

Communicated by K X Tang.

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Ruiz-Ramírez, L.A., del Carmen Godoy-Hernández, G., Álvarez-Gutiérrez, P.E. et al. Protocol for bonediol production in Bonellia macrocarpa hairy root culture. Plant Cell Tiss Organ Cult 134, 177–181 (2018). https://doi.org/10.1007/s11240-018-1403-z

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  • DOI: https://doi.org/10.1007/s11240-018-1403-z

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