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Hairy root induction and Farnesiferol B production of endemic medicinal plant Ferula pseudalliacea

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Abstract

The effects of medium, gibberellic acid (GA3) and stratification treatments on the seed germination of Ferula pseudalliacea were evaluated. Filter paper medium, 500 micro molar GA3 and 8 week chilling treatment were resulted in significantly more seed germination than others. F. pseudalliacea was also transformed by Agrobacterium rhizogenes. Explants from young leaves, stems, cotyledon, and embryo were inoculated with A. rhizogenes strains ATCC 15834, 1724, A4, LB9402 and Ar318. Hairy roots were induced only from 10 to 12-days embryo explants using strains ATCC 15824 and 1724. Although, the transformation efficiency of ATCC 15834 (4%) strain was higher than 1724 (2%). Maximum hairy root transformation frequency (25%) was obtained in infection time of 10 min compared to that of 20 (20%) and 30 (5%) min. In addition, the transformation rate was significantly higher at the inoculation time of 72 h (29%) compared to that of 48 h (22%) and 24 h (6%). Transgenic hairy root lines were confirmed by PCR amplification of rolB gene. Hairy root lines were produced higher biomass in half B5 medium compared to that of half MS medium. Hairy roots lines from the strain ATCC 15834 produced more hairy root numbers and fresh and dried biomass compared to that of the strain 1724. Analyses of transgenic hairy root and natural roots extracts using HPLC showed that all the hairy root lines produced farnesiferol B.

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Acknowledgements

This work was supported by the University of Kurdistan. The authors would like to thank Dr. Mirzaghaderi, University of Kurdistan, for his help in counting of mitotic chromosomes.

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, University of Kurdistan, 416, Sanandaj, Iran

    Abedin Khazaei, Bahman Bahramnejad & Sima Mohammadi

  2. Department of Horticultural Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

    Ali-Akbar Mozafari

  3. Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

    Dara Dastan

  4. Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran

    Dara Dastan

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  1. Abedin Khazaei
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  2. Bahman Bahramnejad
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Correspondence to Bahman Bahramnejad.

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Khazaei, A., Bahramnejad, B., Mozafari, AA. et al. Hairy root induction and Farnesiferol B production of endemic medicinal plant Ferula pseudalliacea. 3 Biotech 9, 407 (2019). https://doi.org/10.1007/s13205-019-1935-x

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