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Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation

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Abstract

Suspension-cultured Dracocephlum polychaetum Bornm. is a wild medicinal herb native to Iran, treated with a static magnetic field (SMF) and Fe2O3 magnetic nanoparticles (MNP). The effect of SMF (30 mT), Fe2O3 MNP (100 ppm) and the combination of these treatments on phenolic metabolism and the medicinal compounds were examined by high performance liquid chromatography and UV–Vis spectrophotometer. The activity of polyphenol oxidase and phenylalanine ammonialyase, as well as the content of total phenolics, flavonoid, anthocyanins, lignin and malondialdehyde in all treated cells, showed a significant difference with control. The intracellular content of rosmarinic acid, naringin, apigenin, thymol, carvacrol, quercetin and rutin, in all treated cells increased considerably. Their quantities also increased significantly in the treated cell culture media. These results suggest this increase is due to the increased production and secretion of the medicinal compounds from cells to the culture media upon application of SMF and Fe2O3 MNP.

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Abbreviations

MNP:

Fe2O3 magnetic nanoparticles

SMF:

Static magnetic field

PPO:

Polyphenol oxidase

PAL:

Phenylalanine ammonia lyase

MDA:

Malondialdehyde

References

  • Aladjadjiyan A (2010) Influence of stationary magnetic field on lentil seeds. Int Agrophys 24:321–324

    Google Scholar 

  • Araji S et al (2014) Novel roles for the polyphenol oxidase enzyme in secondary metabolism and the regulation of cell death in walnut. Plant Physiol 164:1191–1203

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Barreca D, Laganà G, Leuzzi U, Smeriglio A, Trombetta D, Bellocco E (2016) Evaluation of the nutraceutical, antioxidant and cytoprotective properties of ripe pistachio (Pistacia vera L., variety Bronte). Hulls Food Chem 196:493–502

    Article  CAS  PubMed  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Çelik Ö, Büyükuslu N, Atak Ç, Rzakoulieva A (2009) Effects of magnetic field on activity of superoxide dismutase and catalase in Glycine max (L.) Merr. Roots. Pol J Environ Stud 18:175–182

    Google Scholar 

  • Chandra S, Chakraborty N, Dasgupta A, Sarkar J, Panda K, Acharya K (2015) Chitosan nanoparticles: a positive modulator of innate immune responses in plants. Sci Rep 5:15195

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chua LS, Latiff NA, Lee SY, Lee CT, Sarmidi MR, Aziz RA (2011) Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah). Food Chem 127:1186–1192

    Article  CAS  PubMed  Google Scholar 

  • Dias MI, Sousa MJ, Alves RC, Ferreira IC (2016) Exploring plant tissue culture to improve the production of phenolic compounds: a review. Ind Crops Prod 82:9–22

    Article  CAS  Google Scholar 

  • Dimkpa CO et al (2012) CuO and ZnO nanoparticles: phytotoxicity, metal speciation, and induction of oxidative stress in sand-grown wheat. J Nanopart Res 14:1125

    Article  CAS  Google Scholar 

  • Dong J, Ma X, Wei Q, Peng S, Zhang S (2011) Effects of growing location on the contents of secondary metabolites in the leaves of four selected superior clones of Eucommia ulmoides. Ind Crops Prod 34:1607–1614

    Article  CAS  Google Scholar 

  • Galland P, Pazur A (2005) Magnetoreception in plants. J Plant Res 118:371–389

    Article  PubMed  Google Scholar 

  • Gamborg O, Murashige T, Thorpe T, Vasil I (1976) Plant tissue culture media In vitro 12:473–478

    Article  CAS  PubMed  Google Scholar 

  • Ghanati F, Morita A, Yokota H (2005) Effects of aluminum on the growth of tea plant and activation of antioxidant system. Plant Soil 276:133–141

    Article  CAS  Google Scholar 

  • Hara M, Oki K, Hoshino K, Kuboi T (2003) Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus). Hypocotyl Plant Sci 164:259–265

    Article  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  CAS  Google Scholar 

  • Heidarabadi MD, Ghanati F, Fujiwara T (2011) Interaction between boron and aluminum and their effects on phenolic metabolism of Linum usitatissimum L. roots. Plant Physiol Biochem 49:1377–1383

    Article  CAS  PubMed  Google Scholar 

  • Iiyama K, Wallis AF (1990) Determination of lignin in herbaceous plants by an improved acetyl bromide procedure. J Sci Food Agric 51:145–161

    Article  CAS  Google Scholar 

  • Jamshidi M, Ghanati F, Rezaei A, Bemani E (2016) Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs. Cytotechnology 68:525–530

    Article  CAS  PubMed  Google Scholar 

  • Kahn V (1975) Polyphenol oxidase activity and browning of three avocado varieties. J Sci Food Agric 26:1319–1324

    Article  CAS  Google Scholar 

  • Khodami M, Abbasnejad M, Sheibani V, Mobasher M, Mehrabani M, Anaie Goodary A, Salari S (2011) Evaluation of the analgesic and anxiolytic effects of Dracocephalum polychaetum. Physiol Pharmacol 15:444–454

    Google Scholar 

  • Krishnaraj C, Jagan E, Ramachandran R, Abirami S, Mohan N, Kalaichelvan P (2012) Effect of biologically synthesized silver nanoparticles on Bacopa monnieri. (Linn.) Wettst plant growth metabolism. Process Biochem 47:651–658

    Article  CAS  Google Scholar 

  • Ma X, Geiser-Lee J, Deng Y, Kolmakov A (2010) Interactions between engineered nanoparticles (ENPs) and plants: phytotoxicity, uptake and accumulation. Sci Total Environ 408:3053–3061

    Article  CAS  PubMed  Google Scholar 

  • Maffei ME (2014) Magnetic field effects on plant growth, development, and evolution. Front Plant Sci 5:445

    Article  PubMed  PubMed Central  Google Scholar 

  • Morita A, Yokota H, Ishka MR, Ghanati F (2006) Changes in peroxidase activity and lignin content of cultured tea cells in response to excess manganese. Soil Sci Plant Nutr 52:26–31

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Pandya SR, Singh M (2015) Dispersion and optical activities of newly synthesized magnetic nanoparticles with organic acids and dendrimers in DMSO studied with UV/vis spectrophotometry. J Mol Liq 211:146–156

    Article  CAS  Google Scholar 

  • Payez A, Ghanati F, Behmanesh M, Abdolmaleki P, Hajnorouzi A, Rajabbeigi E (2013) Increase of seed germination, growth and membrane integrity of wheat seedlings by exposure to static and a 10-KHz. Electromagn Field Electromagn Biol Med 32:417–429

    Article  CAS  PubMed  Google Scholar 

  • Peñuelas J, Llusià J (1997) Effects of carbon dioxide, water supply, and seasonality on terpene content and emission by Rosmarinus officinalis. J Chem Ecol 23:979–993

    Article  Google Scholar 

  • Petrussa E, Braidot E, Zancani M, Peresson C, Bertolini A, Patui S, Vianello A (2013) Plant flavonoids—biosynthesis, transport and involvement in stress responses. Int J Mol Sci 14:14950–14973

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Poinapen D, Toppozini L, Dies H, Brown DC, Rheinstädter MC (2013) Static magnetic fields enhance lipid order in native plant plasma membrane. Soft Matter 9:6804–6813

    Article  CAS  Google Scholar 

  • Pouraboli I, Nazari S, Sabet N, Sharififar F, Jafari M (2016) Antidiabetic, antioxidant, and antilipid peroxidative activities of Dracocephalum polychaetum shoot extract in streptozotocin-induced diabetic rats: in vivo and in vitro studies. Pharm Biol 54:272–278

    Article  CAS  PubMed  Google Scholar 

  • Rezaei A, Ghanati F, Behmanesh M (2010) Static magnetic field improved salicylic acid effect on taxol production in suspension-cultured hazel (Corylus avellana) cells. In: 6th International workshop on biological effects of electromagnetic fields pp 70–71

  • Sahebjamei H, Abdolmaleki P, Ghanati F (2007) Effects of magnetic field on the antioxidant enzyme activities of suspension-cultured. Tob Cells Bioelectromagn 28:42–47

    Article  CAS  Google Scholar 

  • Shalaby S, Horwitz BA (2015) Plant phenolic compounds and oxidative stress: integrated signals in fungal–plant interactions. Curr Genet 61:347–357

    Article  CAS  PubMed  Google Scholar 

  • Shang G-M, Wu J-C, Yuan Y-J (2004) Improved cell growth and Taxol production of suspension-cultured Taxus chinensis var. mairei in alternating direct current magnetic fields. Biotechnol Lett 26:875–878

    Article  CAS  PubMed  Google Scholar 

  • Shokrollahi S, Ghanati F, Sajedi RH, Sharifi M (2018) Possible role of iron containing proteins in physiological responses of soybean to static magnetic field. J Plant Physiol 226:163–171

    Article  CAS  PubMed  Google Scholar 

  • Simaei M, Khavari-Nejad R, Bernard F (2012) Exogenous application of salicylic acid and nitric oxide on the ionic contents and enzymatic activities in NaCl-stressed soybean plants American. J Plant Sci 3:1495

    Article  CAS  Google Scholar 

  • Tahsili J, Sharifi M, Safaie N, Esmaeilzadeh-Bahabadi S, Behmanesh M (2014) Induction of lignans and phenolic compounds in cell culture of Linum album by culture filtrate of Fusarium graminearum. J Plant Interact 9:412–417

    Article  Google Scholar 

  • Wang JW, Zheng LP, Wu JY, Tan RX (2006) Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide 15:351–358

    Article  CAS  PubMed  Google Scholar 

  • Zhang B, Zheng LP, Yi Li W, Wen Wang J (2013) Stimulation of artemisinin production in Artemisia annua hairy roots by Ag-SiO2 core-shell nanoparticles. Curr Nanosci 9:363–370

    Article  CAS  Google Scholar 

  • Złotek U, Świeca M, Jakubczyk A (2014) Effect of abiotic elicitation on main health-promoting compounds, antioxidant activity and commercial quality of butter lettuce (Lactuca sativa L.). Food Chem 148:253–260

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This study was supported by Shahid Bahonar University of Kerman and Tarbiat Modares University of Tehran, Iran.

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Authors and Affiliations

  1. Department of Plant Biology, Shahid Bahonar University of Kerman, Kerman, Iran

    Marzieh Taghizadeh, Fatemeh Nasibi & Khosrow Manouchehri Kalantari

  2. Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, Kerman, Iran

    Fatemeh Nasibi

  3. Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran

    Faezeh Ghanati

Authors
  1. Marzieh Taghizadeh
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  2. Fatemeh Nasibi
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  3. Khosrow Manouchehri Kalantari
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  4. Faezeh Ghanati
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Contributions

MT and FN devised the project, the main conceptual ideas and proof outline. MT and FGh developed the theory. MT carried out the experiment and collected data. MT verified the analytical methods, and. analyzed and interpreted the data. MT wrote the manuscript. FN and KhMK. contributed to the final version of the manuscript. All authors supervised the project and discussed the results and contributed to the final manuscript.

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Correspondence to Fatemeh Nasibi.

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The authors declare that they have no conflict of interest.

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Communicated by Sergey V. Dolgov.

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Taghizadeh, M., Nasibi, F., Kalantari, K.M. et al. Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation. Plant Cell Tiss Organ Cult 136, 489–498 (2019). https://doi.org/10.1007/s11240-018-01530-1

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  • DOI: https://doi.org/10.1007/s11240-018-01530-1

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