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Production of doubled haploid plants from anther cultures of borage (Borago officinalis L.) by the application of chemical and physical stress

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Abstract

Anther culture can be used as a powerful tool to produce doubled haploid (DH) lines in medicinal plants, thus accelerating breeding programs. In the particular case of borage (Borago officinalis L.), a method to produce DH plants has not been yet published. In this work we evaluated the effect of different culture media and of different chemical (colchicine and n-butanol) and physical stresses (centrifugation and electroporation) on androgenesis induction and plant regeneration in borage anther cultures. We found that the highest response can be obtained with culture medium containing B5 salts and NLN vitamins, the addition of 200 mg/l colchicine during 4 days, a pretreatment of anthers with 0.2% n-butanol for 5 h, or the application to anthers of single physical stresses (either centrifugation at 300×g or a 100 V electrical shock, but not combined). This is the first report on the production of DH plants in borage. Together, the results presented hereby can be used as a basic framework for large-scale generation of DH plants in this important medicinal species.

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, P. O. Box 657833131, Hamedan, Iran

    Zahra Sanaey Hoveida, Mohammad Reza Abdollahi & Sayyed Saeed Moosavi

  2. Department of Agricultural Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

    Asghar Mirzaie-Asl

  3. Cell Biology Group - COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Seguí-Simarro

Authors
  1. Zahra Sanaey Hoveida
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  2. Mohammad Reza Abdollahi
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  3. Asghar Mirzaie-Asl
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  4. Sayyed Saeed Moosavi
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  5. Jose M. Seguí-Simarro
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Corresponding author

Correspondence to Mohammad Reza Abdollahi.

Additional information

Communicated by Sergio J Ochatt.

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Hoveida, Z.S., Abdollahi, M.R., Mirzaie-Asl, A. et al. Production of doubled haploid plants from anther cultures of borage (Borago officinalis L.) by the application of chemical and physical stress. Plant Cell Tiss Organ Cult 130, 369–378 (2017). https://doi.org/10.1007/s11240-017-1233-4

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  • DOI: https://doi.org/10.1007/s11240-017-1233-4

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